Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice
Two independent experiments were performed with three groups each (sepsis control, sepsis, and sepsis with apoE23 treatment) to investigate the anti-inflammatory effect of apolipoprotein 23 (apoE23) in a mouse model of sepsis induced by S. typhimurium. Survival rates; plasma level variations in tumo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-07-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2023-0767 |
| _version_ | 1797768139925094400 |
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| author | Wang Chuanqing Yin Lijun Fu Pan Lu Guoping Zhai Xiaowen Yang Changsheng |
| author_facet | Wang Chuanqing Yin Lijun Fu Pan Lu Guoping Zhai Xiaowen Yang Changsheng |
| author_sort | Wang Chuanqing |
| collection | DOAJ |
| description | Two independent experiments were performed with three groups each (sepsis control, sepsis, and sepsis with apoE23 treatment) to investigate the anti-inflammatory effect of apolipoprotein 23 (apoE23) in a mouse model of sepsis induced by S. typhimurium. Survival rates; plasma level variations in tumor necrosis factor (TNF)-α, interleukin (IL)-6, and lipopolysaccharide (LPS); S. typhimurium colony-forming units in the spleen tissue; and mRNA and protein expression levels of low-density lipoprotein receptor (LDLR), LDLR-related protein (LRP), syndecan-1, and scavenger receptor B1 were evaluated in the livers of mice from the three groups. Results found that the survival rate of septic mice treated with apoE23 was 100% within 48 h, while it was only 40% in septic mice without apoE23 treatment (P < 0.001). The plasma LPS, TNF-α, and IL-6 levels and the S. typhimurium load in mice in the apoE23-treated group were significantly lower than those in septic mice (P < 0.05). Moreover, apoE23 restored the downregulated expression of LDLR and LRP in the liver tissue of septic mice. So apoE23 exhibits an anti-inflammatory effect in the mouse model of S. typhimurium-induced sepsis. Further studies are required to understand the mechanisms underlying the anti-inflammatory effects of apoE23. |
| first_indexed | 2024-03-12T20:50:12Z |
| format | Article |
| id | doaj.art-7bfb6ce70cdd4533b5229726a857a30e |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-03-12T20:50:12Z |
| publishDate | 2023-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-7bfb6ce70cdd4533b5229726a857a30e2023-08-01T05:15:20ZengDe GruyterOpen Medicine2391-54632023-07-011818011010.1515/med-2023-0767Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in miceWang Chuanqing0Yin Lijun1Fu Pan2Lu Guoping3Zhai Xiaowen4Yang Changsheng5Department of Nosocomial Infection Control and the Clinical Microbiology Laboratory, Children’s Hospital of Fudan University, Shanghai200032, ChinaDepartment of Nosocomial Infection Control, Children’s Hospital of Fudan University, Shanghai200032, ChinaDepartment of the Clinical Microbiology Laboratory, Children’s Hospital of Fudan University, Shanghai200032, ChinaDepartment of Pediatric Intensive Care Unit, Children’s Hospital of Fudan University, Shanghai200032, ChinaDepartment of Hematology, Children’s Hospital of Fudan University, Shanghai, 399 Wanyuan Road, Shanghai200032, ChinaThe Institute of Cardiovascular Diseases of Shanghai, Key Laboratory of Viral Heart Diseases, Ministry of Health, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai200032, ChinaTwo independent experiments were performed with three groups each (sepsis control, sepsis, and sepsis with apoE23 treatment) to investigate the anti-inflammatory effect of apolipoprotein 23 (apoE23) in a mouse model of sepsis induced by S. typhimurium. Survival rates; plasma level variations in tumor necrosis factor (TNF)-α, interleukin (IL)-6, and lipopolysaccharide (LPS); S. typhimurium colony-forming units in the spleen tissue; and mRNA and protein expression levels of low-density lipoprotein receptor (LDLR), LDLR-related protein (LRP), syndecan-1, and scavenger receptor B1 were evaluated in the livers of mice from the three groups. Results found that the survival rate of septic mice treated with apoE23 was 100% within 48 h, while it was only 40% in septic mice without apoE23 treatment (P < 0.001). The plasma LPS, TNF-α, and IL-6 levels and the S. typhimurium load in mice in the apoE23-treated group were significantly lower than those in septic mice (P < 0.05). Moreover, apoE23 restored the downregulated expression of LDLR and LRP in the liver tissue of septic mice. So apoE23 exhibits an anti-inflammatory effect in the mouse model of S. typhimurium-induced sepsis. Further studies are required to understand the mechanisms underlying the anti-inflammatory effects of apoE23.https://doi.org/10.1515/med-2023-0767apoe23sepsissalmonella typhimuriumlpsldlrlrp |
| spellingShingle | Wang Chuanqing Yin Lijun Fu Pan Lu Guoping Zhai Xiaowen Yang Changsheng Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice Open Medicine apoe23 sepsis salmonella typhimurium lps ldlr lrp |
| title | Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice |
| title_full | Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice |
| title_fullStr | Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice |
| title_full_unstemmed | Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice |
| title_short | Anti-inflammatory effect of ApoE23 on Salmonella typhimurium-induced sepsis in mice |
| title_sort | anti inflammatory effect of apoe23 on salmonella typhimurium induced sepsis in mice |
| topic | apoe23 sepsis salmonella typhimurium lps ldlr lrp |
| url | https://doi.org/10.1515/med-2023-0767 |
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