<i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure
Arbuscular mycorrhizal fungi (AMF) are essential components of the soil microbiome that can facilitate plant growth and enhance abiotic and biotic stress resistance. However, the mechanisms via which AMF inoculation influences <i>Cucurbita pepo</i> L. plant growth and fruit yield remain...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/1424-2818/14/11/932 |
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| author | Junsong Wang Wenjiang Fu Chenyu Sun Shuai Cai Cheng Tang |
| author_facet | Junsong Wang Wenjiang Fu Chenyu Sun Shuai Cai Cheng Tang |
| author_sort | Junsong Wang |
| collection | DOAJ |
| description | Arbuscular mycorrhizal fungi (AMF) are essential components of the soil microbiome that can facilitate plant growth and enhance abiotic and biotic stress resistance. However, the mechanisms via which AMF inoculation influences <i>Cucurbita pepo</i> L. plant growth and fruit yield remain unclear. Here, we conducted pot experiments to investigate bacterial and fungal community structure in the rhizosphere of <i>C. pepo</i> plants inoculated with <i>Funneliformis mosseae</i> (Nicoll. & Gerd.) Gerd. & Trappe based on 16S ribosomal RNA and internal transcribed spacer gene sequencing. The α-diversity of bacteria increased significantly following <i>F. mosseae</i> inoculation, whereas the α-diversity of fungi exhibited an opposite trend (<i>p</i> < 0.01). The relative abundances of major bacterial phyla, Actinobacteria, Acidobacteria, and Chloroflexi, together with the fungal phylum Ascomycota, were all higher in inoculated samples than in uninoculated controls. <i>F. mosseae</i> inoculation led to remarkable enrichment of potentially beneficial taxa (e.g., <i>Streptomyces</i>, <i>Sphingomonas</i>, <i>Lysobacter</i>, and <i>Trichoderma</i>), in stark contrast to depletion of fungal pathogens (e.g., <i>Botryotrichum</i>, <i>Acremonium</i>, <i>Fusarium</i>, and <i>Plectosphaerella</i>). Pathways related to amino acid metabolism and antibiotic biosynthesis were upregulated by <i>F. mosseae</i> inoculation, whereas pathways involved in infectious diseases were downregulated. The results suggest that <i>F. mosseae</i> inoculation reshapes the rhizosphere microbiome, thereby augmenting <i>C. pepo</i> plant growth and fruit yield. |
| first_indexed | 2024-03-09T19:08:26Z |
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| language | English |
| last_indexed | 2024-03-09T19:08:26Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-7bfbca0e78bb44cea95756ceb58fd15c2023-11-24T04:21:05ZengMDPI AGDiversity1424-28182022-10-01141193210.3390/d14110932<i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community StructureJunsong Wang0Wenjiang Fu1Chenyu Sun2Shuai Cai3Cheng Tang4Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, Yunnan Research Academy of Eco-Environmental Science, Kunming 650034, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Yangling 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Yangling 712100, ChinaChangdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Changdao 265800, ChinaYunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, Yunnan Research Academy of Eco-Environmental Science, Kunming 650034, ChinaArbuscular mycorrhizal fungi (AMF) are essential components of the soil microbiome that can facilitate plant growth and enhance abiotic and biotic stress resistance. However, the mechanisms via which AMF inoculation influences <i>Cucurbita pepo</i> L. plant growth and fruit yield remain unclear. Here, we conducted pot experiments to investigate bacterial and fungal community structure in the rhizosphere of <i>C. pepo</i> plants inoculated with <i>Funneliformis mosseae</i> (Nicoll. & Gerd.) Gerd. & Trappe based on 16S ribosomal RNA and internal transcribed spacer gene sequencing. The α-diversity of bacteria increased significantly following <i>F. mosseae</i> inoculation, whereas the α-diversity of fungi exhibited an opposite trend (<i>p</i> < 0.01). The relative abundances of major bacterial phyla, Actinobacteria, Acidobacteria, and Chloroflexi, together with the fungal phylum Ascomycota, were all higher in inoculated samples than in uninoculated controls. <i>F. mosseae</i> inoculation led to remarkable enrichment of potentially beneficial taxa (e.g., <i>Streptomyces</i>, <i>Sphingomonas</i>, <i>Lysobacter</i>, and <i>Trichoderma</i>), in stark contrast to depletion of fungal pathogens (e.g., <i>Botryotrichum</i>, <i>Acremonium</i>, <i>Fusarium</i>, and <i>Plectosphaerella</i>). Pathways related to amino acid metabolism and antibiotic biosynthesis were upregulated by <i>F. mosseae</i> inoculation, whereas pathways involved in infectious diseases were downregulated. The results suggest that <i>F. mosseae</i> inoculation reshapes the rhizosphere microbiome, thereby augmenting <i>C. pepo</i> plant growth and fruit yield.https://www.mdpi.com/1424-2818/14/11/932arbuscular mycorrhizal fungirhizosphere microbiomebacterial communityfungal community |
| spellingShingle | Junsong Wang Wenjiang Fu Chenyu Sun Shuai Cai Cheng Tang <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure Diversity arbuscular mycorrhizal fungi rhizosphere microbiome bacterial community fungal community |
| title | <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure |
| title_full | <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure |
| title_fullStr | <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure |
| title_full_unstemmed | <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure |
| title_short | <i>Funneliformis mosseae</i> Inoculation Enhances <i>Cucurbita pepo</i> L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure |
| title_sort | i funneliformis mosseae i inoculation enhances i cucurbita pepo i l plant growth and fruit yield by reshaping rhizosphere microbial community structure |
| topic | arbuscular mycorrhizal fungi rhizosphere microbiome bacterial community fungal community |
| url | https://www.mdpi.com/1424-2818/14/11/932 |
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