Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes
Abstract Taxifolin, a bioactive flavonoid, has been attracting attention as a beneficial and valuable phytochemical due to its antioxidant, anticancer, and anti-inflammatory properties. Recently, an improvement effect of taxifolin against psoriasis has been reported in an animal experimental model....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-01-01
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| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-023-00769-3 |
| _version_ | 1811171572402094080 |
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| author | Jung Eun Park Hee Jun Kwon Hwa Jin Lee Hyung Seo Hwang |
| author_facet | Jung Eun Park Hee Jun Kwon Hwa Jin Lee Hyung Seo Hwang |
| author_sort | Jung Eun Park |
| collection | DOAJ |
| description | Abstract Taxifolin, a bioactive flavonoid, has been attracting attention as a beneficial and valuable phytochemical due to its antioxidant, anticancer, and anti-inflammatory properties. Recently, an improvement effect of taxifolin against psoriasis has been reported in an animal experimental model. However, its exact mechanism of action at molecular and cellular levels is not known. Thus, the purpose of this study was to verify the anti-inflammatory effect of taxifolin on psoriasis at cellular/molecular level using HaCaT human keratinocytes. First, a CCK-8 assay was performed to evaluate cytotoxicity of taxifolin. Results revealed that taxifolin was a relatively safe material, showing no cytotoxicity at concentrations up to 300 μg/mL. In TNF-α-induced HaCaT cells, taxifolin significantly inhibited mRNA expression levels of pro-inflammatory cytokines (IL-1α, IL-1-β, and IL-6) and chemokines (CXCL8 and CCL20). The ability of taxifolin to regulation expression of inflammatory cytokine genes was associated with phosphorylation of IκB/STAT3 protein. In addition, taxifolin inhibited expression levels of IL-1α/β, IL-6, CXCL8, and CCL20 by inhibiting IκB/STAT3 protein phosphorylation upon stimulation of TNF-α, IL-17A, and IFN-γ. These results show that taxifolin has the potential to be developed as a treatment for psoriasis and skin inflammation. |
| first_indexed | 2024-04-10T17:16:17Z |
| format | Article |
| id | doaj.art-7c00feb8c9df42d68c8aab014da903f6 |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-04-10T17:16:17Z |
| publishDate | 2023-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-7c00feb8c9df42d68c8aab014da903f62023-02-05T12:21:33ZengSpringerOpenApplied Biological Chemistry2468-08422023-01-016611910.1186/s13765-023-00769-3Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytesJung Eun Park0Hee Jun Kwon1Hwa Jin Lee2Hyung Seo Hwang3School of Cosmetic Science and Beauty Biotechnology, Semyung UniversitySchool of Cosmetic Science and Beauty Biotechnology, Semyung UniversitySchool of Industrial Bio-Pharmaceutical Science, Semyung UniversitySchool of Cosmetic Science and Beauty Biotechnology, Semyung UniversityAbstract Taxifolin, a bioactive flavonoid, has been attracting attention as a beneficial and valuable phytochemical due to its antioxidant, anticancer, and anti-inflammatory properties. Recently, an improvement effect of taxifolin against psoriasis has been reported in an animal experimental model. However, its exact mechanism of action at molecular and cellular levels is not known. Thus, the purpose of this study was to verify the anti-inflammatory effect of taxifolin on psoriasis at cellular/molecular level using HaCaT human keratinocytes. First, a CCK-8 assay was performed to evaluate cytotoxicity of taxifolin. Results revealed that taxifolin was a relatively safe material, showing no cytotoxicity at concentrations up to 300 μg/mL. In TNF-α-induced HaCaT cells, taxifolin significantly inhibited mRNA expression levels of pro-inflammatory cytokines (IL-1α, IL-1-β, and IL-6) and chemokines (CXCL8 and CCL20). The ability of taxifolin to regulation expression of inflammatory cytokine genes was associated with phosphorylation of IκB/STAT3 protein. In addition, taxifolin inhibited expression levels of IL-1α/β, IL-6, CXCL8, and CCL20 by inhibiting IκB/STAT3 protein phosphorylation upon stimulation of TNF-α, IL-17A, and IFN-γ. These results show that taxifolin has the potential to be developed as a treatment for psoriasis and skin inflammation.https://doi.org/10.1186/s13765-023-00769-3Anti-inflammationHaCaTSTAT3PsoriasisTaxifolin |
| spellingShingle | Jung Eun Park Hee Jun Kwon Hwa Jin Lee Hyung Seo Hwang Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes Applied Biological Chemistry Anti-inflammation HaCaT STAT3 Psoriasis Taxifolin |
| title | Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes |
| title_full | Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes |
| title_fullStr | Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes |
| title_full_unstemmed | Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes |
| title_short | Anti-inflammatory effect of taxifolin in TNF-α/IL-17A/IFN-γ induced HaCaT human keratinocytes |
| title_sort | anti inflammatory effect of taxifolin in tnf α il 17a ifn γ induced hacat human keratinocytes |
| topic | Anti-inflammation HaCaT STAT3 Psoriasis Taxifolin |
| url | https://doi.org/10.1186/s13765-023-00769-3 |
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