Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety
The WRKY transcription factor plays a crucial role in plant stress adaptation. Our research has found that WRKY6 in <i>Solanum tuberosum</i> (potatoes) is closely related to cadmium (Cd) tolerance. Therefore, investigating the mechanism of StWRKY6 in plant resistance to Cd toxicity is of...
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MDPI AG
2023-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/12/2303 |
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| author | Guandi He Muhammad Saleem Tingfei Deng Zhuoyan Zhong Tengbing He Jiahai Wu |
| author_facet | Guandi He Muhammad Saleem Tingfei Deng Zhuoyan Zhong Tengbing He Jiahai Wu |
| author_sort | Guandi He |
| collection | DOAJ |
| description | The WRKY transcription factor plays a crucial role in plant stress adaptation. Our research has found that WRKY6 in <i>Solanum tuberosum</i> (potatoes) is closely related to cadmium (Cd) tolerance. Therefore, investigating the mechanism of StWRKY6 in plant resistance to Cd toxicity is of great scientific importance for food safety. This research further analyzed the gene structure and functional regions of the nuclear transcription factor WRKY6 in potatoes, discovering that StWRKY6 contains W box, GB/box, ABRE, and other elements that can act as a nuclear transcription regulatory factor to execute multiple functional regulations. The results of the heterologous expression of StWRKY6 in Arabidopsis under Cd stress showed that the overexpression line (StWRKY6-OE) had significantly higher SAPD values and content of reactive oxygen species scavenging enzymes than the wild type, indicating that StWRKY6 plays a crucial role in protecting the photosynthetic system and promoting carbohydrate synthesis. Transcriptome analysis also revealed that the Cd-induced expression of StWRKY6 up-regulated many potential gene targets, including APR2, DFRA, ABCG1, VSP2, ERF013, SAUR64/67, and BBX20, which are involved in Cd chelation (APR2, DFRA), plant defense (VSP2, PDF1.4), toxic substance efflux (ABCG1), light morphology development (BBX20), and auxin signal (SAUR64/67). These genes coordinate the regulation of Cd tolerance in the StWRKY6 overexpression line. In summary, this study identified a potential gene set of the co-expression module of StWRKY6, providing useful evidence for the remediation of Cd-contaminated soil and the genetic breeding of low Cd-accumulating crops, thereby ensuring food safety. |
| first_indexed | 2024-03-11T02:28:49Z |
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| language | English |
| last_indexed | 2024-03-11T02:28:49Z |
| publishDate | 2023-06-01 |
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| series | Foods |
| spelling | doaj.art-7c0163a65e294821b35eaf7c0f29db142023-11-18T10:23:50ZengMDPI AGFoods2304-81582023-06-011212230310.3390/foods12122303Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food SafetyGuandi He0Muhammad Saleem1Tingfei Deng2Zhuoyan Zhong3Tengbing He4Jiahai Wu5College of Agriculture, Guizhou University, Guiyang 550025, ChinaDepartment of Biological Sciences, Alabama State University, Office 314, 1627 Harris Way, Montgomery, AL 36104, USANational Products Research Center of Guizhou Province, Guiyang 550025, ChinaCollege of Agriculture, Guizhou University, Guiyang 550025, ChinaCollege of Agriculture, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Academy of Agricultural Sciences, Animal Husbandry and Veterinary Research Institute, Guiyang 550005, ChinaThe WRKY transcription factor plays a crucial role in plant stress adaptation. Our research has found that WRKY6 in <i>Solanum tuberosum</i> (potatoes) is closely related to cadmium (Cd) tolerance. Therefore, investigating the mechanism of StWRKY6 in plant resistance to Cd toxicity is of great scientific importance for food safety. This research further analyzed the gene structure and functional regions of the nuclear transcription factor WRKY6 in potatoes, discovering that StWRKY6 contains W box, GB/box, ABRE, and other elements that can act as a nuclear transcription regulatory factor to execute multiple functional regulations. The results of the heterologous expression of StWRKY6 in Arabidopsis under Cd stress showed that the overexpression line (StWRKY6-OE) had significantly higher SAPD values and content of reactive oxygen species scavenging enzymes than the wild type, indicating that StWRKY6 plays a crucial role in protecting the photosynthetic system and promoting carbohydrate synthesis. Transcriptome analysis also revealed that the Cd-induced expression of StWRKY6 up-regulated many potential gene targets, including APR2, DFRA, ABCG1, VSP2, ERF013, SAUR64/67, and BBX20, which are involved in Cd chelation (APR2, DFRA), plant defense (VSP2, PDF1.4), toxic substance efflux (ABCG1), light morphology development (BBX20), and auxin signal (SAUR64/67). These genes coordinate the regulation of Cd tolerance in the StWRKY6 overexpression line. In summary, this study identified a potential gene set of the co-expression module of StWRKY6, providing useful evidence for the remediation of Cd-contaminated soil and the genetic breeding of low Cd-accumulating crops, thereby ensuring food safety.https://www.mdpi.com/2304-8158/12/12/2303plant stress adaptationWRKY transcription factorreactive oxygen species scavenging enzymeCd-contaminated soilcrop genetic resources |
| spellingShingle | Guandi He Muhammad Saleem Tingfei Deng Zhuoyan Zhong Tengbing He Jiahai Wu Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety Foods plant stress adaptation WRKY transcription factor reactive oxygen species scavenging enzyme Cd-contaminated soil crop genetic resources |
| title | Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety |
| title_full | Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety |
| title_fullStr | Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety |
| title_full_unstemmed | Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety |
| title_short | Unraveling the Mechanism of StWRKY6 in Potato (<i>Solanum tuberosum</i>)’s Cadmium Tolerance for Ensuring Food Safety |
| title_sort | unraveling the mechanism of stwrky6 in potato i solanum tuberosum i s cadmium tolerance for ensuring food safety |
| topic | plant stress adaptation WRKY transcription factor reactive oxygen species scavenging enzyme Cd-contaminated soil crop genetic resources |
| url | https://www.mdpi.com/2304-8158/12/12/2303 |
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