Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study
Glial neoplasms are a group of diseases with poor prognoses. Not all risk factors are known, and no screening tests are available. Only histology provides certain diagnosis. As already reported, DNA transported by exosomes can be an excellent source of information shared by cells locally or systemic...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/12/4/500 |
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| author | Amedeo Piazza Paolo Rosa Luca Ricciardi Antonella Mangraviti Luca Pacini Antonella Calogero Antonino Raco Massimo Miscusi |
| author_facet | Amedeo Piazza Paolo Rosa Luca Ricciardi Antonella Mangraviti Luca Pacini Antonella Calogero Antonino Raco Massimo Miscusi |
| author_sort | Amedeo Piazza |
| collection | DOAJ |
| description | Glial neoplasms are a group of diseases with poor prognoses. Not all risk factors are known, and no screening tests are available. Only histology provides certain diagnosis. As already reported, DNA transported by exosomes can be an excellent source of information shared by cells locally or systemically. These vesicles seem to be one of the main mechanisms of tumor remote intercellular signaling used to induce immune deregulation, apoptosis, and both phenotypic and genotypic modifications. In this study, we evaluated the exosomal DNA (exoDNA) concentration in blood samples of patients affected by cerebral glioma and correlated it with histological and radiological characteristics of tumors. From 14 patients with diagnosed primary or recurrent glioma, we obtained MRI imaging data, histological data, and preoperative blood samples that were used to extract circulating exosomal DNA, which we then quantified. Our results demonstrate a relationship between the amount of circulating exosomal DNA and tumor volume, and mitotic activity. In particular, a high concentration of exoDNA was noted in low-grade gliomas. Our results suggest a possible role of exoDNAs in the diagnosis of brain glioma. They could be particularly useful in detecting early recurrent high-grade gliomas and asymptomatic low-grade gliomas. |
| first_indexed | 2024-03-09T11:05:13Z |
| format | Article |
| id | doaj.art-7c03ce8ff9c44234a980e429873020b2 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-09T11:05:13Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-7c03ce8ff9c44234a980e429873020b22023-12-01T01:00:18ZengMDPI AGBrain Sciences2076-34252022-04-0112450010.3390/brainsci12040500Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary StudyAmedeo Piazza0Paolo Rosa1Luca Ricciardi2Antonella Mangraviti3Luca Pacini4Antonella Calogero5Antonino Raco6Massimo Miscusi7Operative Unit of Neurosurgery, Department of NESMOS, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyOperative Unit of Neurosurgery, Department of NESMOS, Sapienza University of Rome, 00185 Rome, ItalyOperative Unit of Neurosurgery, Department of NESMOS, Sapienza University of Rome, 00185 Rome, ItalyPathology Unit, I.C.O.T. Hospital, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyOperative Unit of Neurosurgery, Department of NESMOS, Sapienza University of Rome, 00185 Rome, ItalyOperative Unit of Neurosurgery, Department of NESMOS, Sapienza University of Rome, 00185 Rome, ItalyGlial neoplasms are a group of diseases with poor prognoses. Not all risk factors are known, and no screening tests are available. Only histology provides certain diagnosis. As already reported, DNA transported by exosomes can be an excellent source of information shared by cells locally or systemically. These vesicles seem to be one of the main mechanisms of tumor remote intercellular signaling used to induce immune deregulation, apoptosis, and both phenotypic and genotypic modifications. In this study, we evaluated the exosomal DNA (exoDNA) concentration in blood samples of patients affected by cerebral glioma and correlated it with histological and radiological characteristics of tumors. From 14 patients with diagnosed primary or recurrent glioma, we obtained MRI imaging data, histological data, and preoperative blood samples that were used to extract circulating exosomal DNA, which we then quantified. Our results demonstrate a relationship between the amount of circulating exosomal DNA and tumor volume, and mitotic activity. In particular, a high concentration of exoDNA was noted in low-grade gliomas. Our results suggest a possible role of exoDNAs in the diagnosis of brain glioma. They could be particularly useful in detecting early recurrent high-grade gliomas and asymptomatic low-grade gliomas.https://www.mdpi.com/2076-3425/12/4/500gliomaexosomeliquid biopsyEVs |
| spellingShingle | Amedeo Piazza Paolo Rosa Luca Ricciardi Antonella Mangraviti Luca Pacini Antonella Calogero Antonino Raco Massimo Miscusi Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study Brain Sciences glioma exosome liquid biopsy EVs |
| title | Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study |
| title_full | Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study |
| title_fullStr | Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study |
| title_full_unstemmed | Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study |
| title_short | Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study |
| title_sort | circulating exosomal dna in glioma patients a quantitative study and histopathological correlations a preliminary study |
| topic | glioma exosome liquid biopsy EVs |
| url | https://www.mdpi.com/2076-3425/12/4/500 |
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