GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma
BackgroundGlioma is a lethal primary tumor of central nervous system. Ferroptosis is a newly identified form of necrotic cell death. Triggering ferroptosis has shown potential to eliminate aggressive tumors. GPX7, a member of glutathione peroxidase family (GPXs), has been described to participate in...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.802124/full |
| _version_ | 1818744569295011840 |
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| author | Yan Zhou Haiyang Wu Fanchen Wang Lixia Xu Yan Yan Xiaoguang Tong Xiaoguang Tong Hua Yan Hua Yan |
| author_facet | Yan Zhou Haiyang Wu Fanchen Wang Lixia Xu Yan Yan Xiaoguang Tong Xiaoguang Tong Hua Yan Hua Yan |
| author_sort | Yan Zhou |
| collection | DOAJ |
| description | BackgroundGlioma is a lethal primary tumor of central nervous system. Ferroptosis is a newly identified form of necrotic cell death. Triggering ferroptosis has shown potential to eliminate aggressive tumors. GPX7, a member of glutathione peroxidase family (GPXs), has been described to participate in oxidative stress and tumorigenesis. However, the biological functions of GPX7 in glioma are still unknown.MethodsBioinformatics method was used to assess the prognostic role of GPX7 in glioma. CCK8, wound healing, transwell and cell apoptosis assays were performed to explore the functions of GPX7 in glioma cells. In vivo experiment was also conducted to confirm in vitro findings. Ferroptosis-related assays were carried out to investigate the association between GPX7 and ferroptosis in glioma.ResultsGPX7 was aberrantly expressed in glioma and higher expression of GPX7 was correlated with adverse outcomes. GPX7 silencing enhanced ferroptosis-related oxidative stress in glioma cells and the loss of GXP7 sensitized glioma to ferroptosis induced by erastin. Furthermore, we found that miR-29b directly suppressed GPX7 expression post-transcriptionally. Reconstitution of miR-29b enhanced erastin sensitivity, partly via GPX7 suppression.ConclusionsOur study clarified the prognostic role of GPX7 in glioma and preliminarily revealed the role of GPX7 in ferroptosis, which may be conducive to the exploration of therapeutic targets of glioma. |
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| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-18T02:46:23Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-7c03fed749274533b870868a30194ba12022-12-21T21:23:34ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-01-011110.3389/fonc.2021.802124802124GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in GliomaYan Zhou0Haiyang Wu1Fanchen Wang2Lixia Xu3Yan Yan4Xiaoguang Tong5Xiaoguang Tong6Hua Yan7Hua Yan8Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, ChinaClinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, ChinaClinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, ChinaTianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, Tianjin, ChinaClinical Laboratory, Tianjin Huanhu Hospital, Tianjin, ChinaClinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, ChinaTianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, Tianjin, ChinaClinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, ChinaTianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, Tianjin, ChinaBackgroundGlioma is a lethal primary tumor of central nervous system. Ferroptosis is a newly identified form of necrotic cell death. Triggering ferroptosis has shown potential to eliminate aggressive tumors. GPX7, a member of glutathione peroxidase family (GPXs), has been described to participate in oxidative stress and tumorigenesis. However, the biological functions of GPX7 in glioma are still unknown.MethodsBioinformatics method was used to assess the prognostic role of GPX7 in glioma. CCK8, wound healing, transwell and cell apoptosis assays were performed to explore the functions of GPX7 in glioma cells. In vivo experiment was also conducted to confirm in vitro findings. Ferroptosis-related assays were carried out to investigate the association between GPX7 and ferroptosis in glioma.ResultsGPX7 was aberrantly expressed in glioma and higher expression of GPX7 was correlated with adverse outcomes. GPX7 silencing enhanced ferroptosis-related oxidative stress in glioma cells and the loss of GXP7 sensitized glioma to ferroptosis induced by erastin. Furthermore, we found that miR-29b directly suppressed GPX7 expression post-transcriptionally. Reconstitution of miR-29b enhanced erastin sensitivity, partly via GPX7 suppression.ConclusionsOur study clarified the prognostic role of GPX7 in glioma and preliminarily revealed the role of GPX7 in ferroptosis, which may be conducive to the exploration of therapeutic targets of glioma.https://www.frontiersin.org/articles/10.3389/fonc.2021.802124/fullbioinformaticsferroptosisgliomaGPX7miR-29b |
| spellingShingle | Yan Zhou Haiyang Wu Fanchen Wang Lixia Xu Yan Yan Xiaoguang Tong Xiaoguang Tong Hua Yan Hua Yan GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma Frontiers in Oncology bioinformatics ferroptosis glioma GPX7 miR-29b |
| title | GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma |
| title_full | GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma |
| title_fullStr | GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma |
| title_full_unstemmed | GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma |
| title_short | GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma |
| title_sort | gpx7 is targeted by mir 29b and gpx7 knockdown enhances ferroptosis induced by erastin in glioma |
| topic | bioinformatics ferroptosis glioma GPX7 miR-29b |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.802124/full |
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