Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar
Introduction: Multidrug resistant tuberculosis (MDR-TB) is caused by Infection with Mycobacterium tuberculosis which is resistant to both isoniazid (INH) and rifampicin (RIF), with or without any other anti tubercular drug. It is caused by resistant mutant strains due to inadequate treatment an...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6793/14024_CE(Ra1)_F(GH)_PF1(BMAK)_PFA(P)_PF2(PAG).pdf |
| _version_ | 1818834162131402752 |
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| author | Sunita Tripathy Rajesh Kumar Surya Deo Singh |
| author_facet | Sunita Tripathy Rajesh Kumar Surya Deo Singh |
| author_sort | Sunita Tripathy |
| collection | DOAJ |
| description | Introduction: Multidrug resistant tuberculosis (MDR-TB) is
caused by Infection with Mycobacterium tuberculosis which
is resistant to both isoniazid (INH) and rifampicin (RIF), with
or without any other anti tubercular drug. It is caused by
resistant mutant strains due to inadequate treatment
and poor compliance. Due to time taking conventional
diagnostic methods, drug resistant strains continue to spread.
Therefore rapid diagnosis and treatment of MDR-TB strains are
prerequisites for the worldwide fight against TB.
Objective: To determine the prevalence of MDR TB in North
Bihar by molecular diagnostic method and to facilitate early
diagnosis and treatment. Also, to find out the number of those
diagnosed cases who were successfully initiated the treatment
in MDR TB Centre of DMCH.
Materials and Methods: This six month observational study
was carried out in IRL Darbhanga, Damien TB research Centre
of the Darbhanga Medical College and Hospital, Bihar, India.
During the period of February-July 2014, 256 sputum samples
were collected from suspected cases of multidrug resistant
tuberculosis, from 6 districts of North Bihar around Darbhanga.
These samples were subjected to routine microscopy and
culture to detect Mycobacterium tuberculosis. Positive cases
were subjected to drug sensitivity test by a molecular diagnostic
method, Using Genotype MTBDR plus kit.
Result: Out of 256 sputum samples from suspected cases of
MDR TB, 122 cases were microscopy positive for tuberculosis.
Among these 122 cases, tuberculosis was confirmed by PCR
in 114 cases. Finally with the help of Line Probe Assay (LPA),
39(15%) samples were found to have resistance to both INH
and Rifampicin. Male female ratio was 4:1.
Conclusion: The Prevalence of Multi drug resistant pulmonary
tuberculosis in North Bihar is 15%. It needs early diagnosis by
molecular diagnostic method and prompt treatment to reduce
the spread of MDR TB cases. |
| first_indexed | 2024-12-19T02:30:26Z |
| format | Article |
| id | doaj.art-7c061cb6e216442b88e798dac978750b |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T02:30:26Z |
| publishDate | 2015-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-7c061cb6e216442b88e798dac978750b2022-12-21T20:39:40ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-11-01911LC09LC1210.7860/JCDR/2015/14024.6793Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North BiharSunita Tripathy0Rajesh Kumar1Surya Deo Singh2Professor, Department of Biochemistry, Darbhanga Medical College, Darbhanga, India.Senior, Medical Officer, DRTB Centre, DMCH, Darbhanga, India.Associate Professor, Department of Preventive and Social Medicine, DMCH, Darbhanga, India.Introduction: Multidrug resistant tuberculosis (MDR-TB) is caused by Infection with Mycobacterium tuberculosis which is resistant to both isoniazid (INH) and rifampicin (RIF), with or without any other anti tubercular drug. It is caused by resistant mutant strains due to inadequate treatment and poor compliance. Due to time taking conventional diagnostic methods, drug resistant strains continue to spread. Therefore rapid diagnosis and treatment of MDR-TB strains are prerequisites for the worldwide fight against TB. Objective: To determine the prevalence of MDR TB in North Bihar by molecular diagnostic method and to facilitate early diagnosis and treatment. Also, to find out the number of those diagnosed cases who were successfully initiated the treatment in MDR TB Centre of DMCH. Materials and Methods: This six month observational study was carried out in IRL Darbhanga, Damien TB research Centre of the Darbhanga Medical College and Hospital, Bihar, India. During the period of February-July 2014, 256 sputum samples were collected from suspected cases of multidrug resistant tuberculosis, from 6 districts of North Bihar around Darbhanga. These samples were subjected to routine microscopy and culture to detect Mycobacterium tuberculosis. Positive cases were subjected to drug sensitivity test by a molecular diagnostic method, Using Genotype MTBDR plus kit. Result: Out of 256 sputum samples from suspected cases of MDR TB, 122 cases were microscopy positive for tuberculosis. Among these 122 cases, tuberculosis was confirmed by PCR in 114 cases. Finally with the help of Line Probe Assay (LPA), 39(15%) samples were found to have resistance to both INH and Rifampicin. Male female ratio was 4:1. Conclusion: The Prevalence of Multi drug resistant pulmonary tuberculosis in North Bihar is 15%. It needs early diagnosis by molecular diagnostic method and prompt treatment to reduce the spread of MDR TB cases.https://jcdr.net/articles/PDF/6793/14024_CE(Ra1)_F(GH)_PF1(BMAK)_PFA(P)_PF2(PAG).pdfdr tb (drug resistant tuberculosis)inh (isoniazide)irl (intermediate referral laboratory)lpa (line probe assay)programmatic management of drug resistant tb (pmdt) |
| spellingShingle | Sunita Tripathy Rajesh Kumar Surya Deo Singh Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar Journal of Clinical and Diagnostic Research dr tb (drug resistant tuberculosis) inh (isoniazide) irl (intermediate referral laboratory) lpa (line probe assay) programmatic management of drug resistant tb (pmdt) |
| title | Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar |
| title_full | Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar |
| title_fullStr | Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar |
| title_full_unstemmed | Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar |
| title_short | Prevalence of Multidrug Resistant Pulmonary Tuberculosis in North Bihar |
| title_sort | prevalence of multidrug resistant pulmonary tuberculosis in north bihar |
| topic | dr tb (drug resistant tuberculosis) inh (isoniazide) irl (intermediate referral laboratory) lpa (line probe assay) programmatic management of drug resistant tb (pmdt) |
| url | https://jcdr.net/articles/PDF/6793/14024_CE(Ra1)_F(GH)_PF1(BMAK)_PFA(P)_PF2(PAG).pdf |
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