Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic ra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1390589 |
| _version_ | 1818107510297460736 |
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| author | Ling-Ling Xia Qi-Jin Zhu Yong-Gui Wu |
| author_facet | Ling-Ling Xia Qi-Jin Zhu Yong-Gui Wu |
| author_sort | Ling-Ling Xia |
| collection | DOAJ |
| description | Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic rats. Objectives: Current study investigates prevention of liver injury by TGP in diabetic rats and its mechanism was related to the inhibition of endoplasmic reticulum stress (ERS). Materials and methods: Fifty adult male rats were randomly divided into: Normal group, diabetic group, TGP (50, 100 and 200 mg/kg/day) treatment groups (n = 10 per group). At the end of the 8th week, the liver was removed for biochemical and histological examinations. Results: Compared with the diabetic group, administration of TGP at doses of 50, 100 and 200 mg/kg significantly prevented the increase of hepatic fibrosis score (ED50 139.4 mg/kg). Compared with diabetic group, TGP at doses of 50, 100 and 200 mg/kg showed an inhibition on the increased macrophage infiltration. MCP-1 and TNF-α mRNA and protein expression were significantly increased in diabetic group compared with normal group; TGP administration caused significant reduction of high levels of MCP-1 and TNF-α mRNA as well as protein levels. Also, TGP at all doses showed an inhibition on the increased GRP78 levels, p-Perk levels and p-Eif2α levels in liver from diabetic group. Discussion and conclusions: Our results indicate that TGP has potential as a treatment for diabetic liver injury attenuating liver lipid accumulation and inflammation as well as ERS induced by diabetic condition. |
| first_indexed | 2024-12-11T02:00:37Z |
| format | Article |
| id | doaj.art-7c06c28b9b9f4af9ae46f7b4b1e8f909 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-11T02:00:37Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-7c06c28b9b9f4af9ae46f7b4b1e8f9092022-12-22T01:24:30ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512178218710.1080/13880209.2017.13905891390589Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic ratsLing-Ling Xia0Qi-Jin Zhu1Yong-Gui Wu2The First Affiliated Hospital, Anhui Medical UniversityThe First Affiliated Hospital, Anhui Medical UniversityThe First Affiliated Hospital, Anhui Medical UniversityContext: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic rats. Objectives: Current study investigates prevention of liver injury by TGP in diabetic rats and its mechanism was related to the inhibition of endoplasmic reticulum stress (ERS). Materials and methods: Fifty adult male rats were randomly divided into: Normal group, diabetic group, TGP (50, 100 and 200 mg/kg/day) treatment groups (n = 10 per group). At the end of the 8th week, the liver was removed for biochemical and histological examinations. Results: Compared with the diabetic group, administration of TGP at doses of 50, 100 and 200 mg/kg significantly prevented the increase of hepatic fibrosis score (ED50 139.4 mg/kg). Compared with diabetic group, TGP at doses of 50, 100 and 200 mg/kg showed an inhibition on the increased macrophage infiltration. MCP-1 and TNF-α mRNA and protein expression were significantly increased in diabetic group compared with normal group; TGP administration caused significant reduction of high levels of MCP-1 and TNF-α mRNA as well as protein levels. Also, TGP at all doses showed an inhibition on the increased GRP78 levels, p-Perk levels and p-Eif2α levels in liver from diabetic group. Discussion and conclusions: Our results indicate that TGP has potential as a treatment for diabetic liver injury attenuating liver lipid accumulation and inflammation as well as ERS induced by diabetic condition.http://dx.doi.org/10.1080/13880209.2017.1390589diabetesliverendoplasmic reticulum stressinflammation |
| spellingShingle | Ling-Ling Xia Qi-Jin Zhu Yong-Gui Wu Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats Pharmaceutical Biology diabetes liver endoplasmic reticulum stress inflammation |
| title | Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| title_full | Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| title_fullStr | Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| title_full_unstemmed | Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| title_short | Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| title_sort | hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats |
| topic | diabetes liver endoplasmic reticulum stress inflammation |
| url | http://dx.doi.org/10.1080/13880209.2017.1390589 |
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