INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA
An in vitro study with Ri-TDNA transformed roots of carrot (Daucus carota) was carried out to evaluate the role of macro-elements contributed as impurities in the gelling agent (phytagel) over and above those present in the minimal (M) medium. Production of root biomass was taken as a measure to qua...
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
1997-01-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Subjects: | |
| Online Access: | http://journal.biotrop.org/index.php/biotropia/article/view/128/99 |
| _version_ | 1818062263604477952 |
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| author | ANJALI VERMA ALOK ADHOLEYA NAVEEN PAL BHATIA |
| author_facet | ANJALI VERMA ALOK ADHOLEYA NAVEEN PAL BHATIA |
| author_sort | ANJALI VERMA |
| collection | DOAJ |
| description | An in vitro study with Ri-TDNA transformed roots of carrot (Daucus carota) was carried out to evaluate the role of macro-elements contributed as impurities in the gelling agent (phytagel) over and above those present in the minimal (M) medium. Production of root biomass was taken as a measure to quantify the influence of macro-elements added to the minimal medium. The levels of phosphorus when adjusted to 1.19 mg/1 and 1.09 mg/l, lead to dry root biomass production at par with the control. Attempts made to lower the amount of impurities in phytagel by de-ionization using different alkalies, proved NaOH to give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum number of infection units and auxiliary cells when phytagel was added to the minimal medium. |
| first_indexed | 2024-12-10T14:01:26Z |
| format | Article |
| id | doaj.art-7c09578c6aed449a947c5fe0dd4dab0d |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-12-10T14:01:26Z |
| publishDate | 1997-01-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-7c09578c6aed449a947c5fe0dd4dab0d2022-12-22T01:45:47ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X1997-01-01106374INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITAANJALI VERMAALOK ADHOLEYANAVEEN PAL BHATIAAn in vitro study with Ri-TDNA transformed roots of carrot (Daucus carota) was carried out to evaluate the role of macro-elements contributed as impurities in the gelling agent (phytagel) over and above those present in the minimal (M) medium. Production of root biomass was taken as a measure to quantify the influence of macro-elements added to the minimal medium. The levels of phosphorus when adjusted to 1.19 mg/1 and 1.09 mg/l, lead to dry root biomass production at par with the control. Attempts made to lower the amount of impurities in phytagel by de-ionization using different alkalies, proved NaOH to give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum number of infection units and auxiliary cells when phytagel was added to the minimal medium.http://journal.biotrop.org/index.php/biotropia/article/view/128/99Agrobacterium rhiiogenesfDaucus caro/a/Gelling agents/diaspora margarita/Macro- elements/Vesicular-arbuscular mycorrhiza/Transformed roots. |
| spellingShingle | ANJALI VERMA ALOK ADHOLEYA NAVEEN PAL BHATIA INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA Biotropia: The Southeast Asian Journal of Tropical Biology Agrobacterium rhiiogenesfDaucus caro/a/Gelling agents/diaspora margarita/Macro- elements/Vesicular-arbuscular mycorrhiza/Transformed roots. |
| title | INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA |
| title_full | INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA |
| title_fullStr | INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA |
| title_full_unstemmed | INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA |
| title_short | INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA |
| title_sort | influence of media gelling agents on root biomass and in vitro va mycorrfflzal symbiosis of carrot with gigaspora margarita |
| topic | Agrobacterium rhiiogenesfDaucus caro/a/Gelling agents/diaspora margarita/Macro- elements/Vesicular-arbuscular mycorrhiza/Transformed roots. |
| url | http://journal.biotrop.org/index.php/biotropia/article/view/128/99 |
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