Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia
Rift Valley fever phlebovirus (RVFV) is a highly pathogenic mosquito-borne virus with bioweapon potential due to its ability to be spread by aerosol transmission. Neurological symptoms are among the worst outcomes of infection, and understanding of pathogenesis mechanisms within the brain is limited...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/2/159 |
| _version_ | 1797297277220421632 |
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| author | Margarita V. Rangel Feliza A. Bourguet Carolyn I. Hall Dina R. Weilhammer |
| author_facet | Margarita V. Rangel Feliza A. Bourguet Carolyn I. Hall Dina R. Weilhammer |
| author_sort | Margarita V. Rangel |
| collection | DOAJ |
| description | Rift Valley fever phlebovirus (RVFV) is a highly pathogenic mosquito-borne virus with bioweapon potential due to its ability to be spread by aerosol transmission. Neurological symptoms are among the worst outcomes of infection, and understanding of pathogenesis mechanisms within the brain is limited. RVFV is classified as an overlap select agent by the CDC and USDA; therefore, experiments involving fully virulent strains of virus are tightly regulated. Here, we present two methods for inactivation of live virus within samples derived from mouse microglia cells using commercially available kits for the preparation of cells for flow cytometry and RNA extraction. Using the flow cytometry protocol, we demonstrate key differences in the response of primary murine microglia to infection with fully virulent versus attenuated RVFV. |
| first_indexed | 2024-03-07T22:18:45Z |
| format | Article |
| id | doaj.art-7c0cc1914e4a4a328b09facf2606df42 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-07T22:18:45Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7c0cc1914e4a4a328b09facf2606df422024-02-23T15:30:23ZengMDPI AGPathogens2076-08172024-02-0113215910.3390/pathogens13020159Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse MicrogliaMargarita V. Rangel0Feliza A. Bourguet1Carolyn I. Hall2Dina R. Weilhammer3Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USABiosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USAEnvironment, Safety & Health, Biosafety & Biogovernance Functional Area, Lawrence Livermore National Laboratory, Livermore, CA 94550, USABiosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USARift Valley fever phlebovirus (RVFV) is a highly pathogenic mosquito-borne virus with bioweapon potential due to its ability to be spread by aerosol transmission. Neurological symptoms are among the worst outcomes of infection, and understanding of pathogenesis mechanisms within the brain is limited. RVFV is classified as an overlap select agent by the CDC and USDA; therefore, experiments involving fully virulent strains of virus are tightly regulated. Here, we present two methods for inactivation of live virus within samples derived from mouse microglia cells using commercially available kits for the preparation of cells for flow cytometry and RNA extraction. Using the flow cytometry protocol, we demonstrate key differences in the response of primary murine microglia to infection with fully virulent versus attenuated RVFV.https://www.mdpi.com/2076-0817/13/2/159Rift Valley fever phlebovirusinactivationselect agentparaformaldehydeguanidine hydrochloridemicroglia |
| spellingShingle | Margarita V. Rangel Feliza A. Bourguet Carolyn I. Hall Dina R. Weilhammer Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia Pathogens Rift Valley fever phlebovirus inactivation select agent paraformaldehyde guanidine hydrochloride microglia |
| title | Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia |
| title_full | Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia |
| title_fullStr | Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia |
| title_full_unstemmed | Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia |
| title_short | Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia |
| title_sort | evaluation of inactivation methods for rift valley fever virus in mouse microglia |
| topic | Rift Valley fever phlebovirus inactivation select agent paraformaldehyde guanidine hydrochloride microglia |
| url | https://www.mdpi.com/2076-0817/13/2/159 |
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