A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells
Cancer is a denying disease and among a number of cancers types, breast cancer is usual in females, and affect the worldwide. For these reason, the work presented here to show the cytological and apoptotic evaluation of breast (MCF-7) cancer cells with functional carbon nanotubes (FCNTs) and rare ea...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723003312 |
| _version_ | 1797653962562732032 |
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| author | Rizwan Wahab Maqsood A.Siddiqui Javed Ahmad Quaiser Saquib Abdulaziz A. Al-Khedhairy |
| author_facet | Rizwan Wahab Maqsood A.Siddiqui Javed Ahmad Quaiser Saquib Abdulaziz A. Al-Khedhairy |
| author_sort | Rizwan Wahab |
| collection | DOAJ |
| description | Cancer is a denying disease and among a number of cancers types, breast cancer is usual in females, and affect the worldwide. For these reason, the work presented here to show the cytological and apoptotic evaluation of breast (MCF-7) cancer cells with functional carbon nanotubes (FCNTs) and rare earth material (REE) neodymium oxide. At initial, the CNTs were functionalized with acid treatment method, whereas neodymium oxide nanorods (Nd2O3NRs) were synthesized and characterized. The Fourier transform infrared (FTIR) was used to check the functional characteristics of CNTs, whereas crystalline property, particle size and phase of Nd2O3NRs were confirmed via X-ray diffraction pattern (XRD) respectively. The morphologies of nanostructures were observed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) correspondingly. The cytological efficiencies of cancer cells were evaluated with FCNTs and Nd2O3NRs at varied concentrations (1, 2, 5, 10, 25, 50 and 100 μg/mL) verified via MTT and NRU assays. The ROS was enhanced in Nd2O3NRs as compared to CNTs with control. The gene expression study were also conducted and it unveils that the level of mRNA with Nd2O3NRs were upregulated and it depicts the apoptosis in cells. The study designates that the Nd2O3NRs are more efficient as compared to the functional CNTs with MCF-7 cancer cell. |
| first_indexed | 2024-03-11T16:52:27Z |
| format | Article |
| id | doaj.art-7c0fdae71e1c4a72b1fe46318c145216 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-11T16:52:27Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-7c0fdae71e1c4a72b1fe46318c1452162023-10-21T04:22:09ZengElsevierJournal of King Saud University: Science1018-36472023-11-01358102869A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cellsRizwan Wahab0Maqsood A.Siddiqui1Javed Ahmad2Quaiser Saquib3Abdulaziz A. Al-Khedhairy4Corresponding author at: College of Science, Zoology Department, P.O. Box 2455, King Saud University, Riyadh 11451, Saudi Arabia.; Chair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaCancer is a denying disease and among a number of cancers types, breast cancer is usual in females, and affect the worldwide. For these reason, the work presented here to show the cytological and apoptotic evaluation of breast (MCF-7) cancer cells with functional carbon nanotubes (FCNTs) and rare earth material (REE) neodymium oxide. At initial, the CNTs were functionalized with acid treatment method, whereas neodymium oxide nanorods (Nd2O3NRs) were synthesized and characterized. The Fourier transform infrared (FTIR) was used to check the functional characteristics of CNTs, whereas crystalline property, particle size and phase of Nd2O3NRs were confirmed via X-ray diffraction pattern (XRD) respectively. The morphologies of nanostructures were observed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) correspondingly. The cytological efficiencies of cancer cells were evaluated with FCNTs and Nd2O3NRs at varied concentrations (1, 2, 5, 10, 25, 50 and 100 μg/mL) verified via MTT and NRU assays. The ROS was enhanced in Nd2O3NRs as compared to CNTs with control. The gene expression study were also conducted and it unveils that the level of mRNA with Nd2O3NRs were upregulated and it depicts the apoptosis in cells. The study designates that the Nd2O3NRs are more efficient as compared to the functional CNTs with MCF-7 cancer cell.http://www.sciencedirect.com/science/article/pii/S1018364723003312Functional CNTsNeodymium oxideMCF-7 cellsROSRT-PCR |
| spellingShingle | Rizwan Wahab Maqsood A.Siddiqui Javed Ahmad Quaiser Saquib Abdulaziz A. Al-Khedhairy A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells Journal of King Saud University: Science Functional CNTs Neodymium oxide MCF-7 cells ROS RT-PCR |
| title | A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| title_full | A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| title_fullStr | A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| title_full_unstemmed | A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| title_short | A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| title_sort | cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells |
| topic | Functional CNTs Neodymium oxide MCF-7 cells ROS RT-PCR |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723003312 |
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