<i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria
This study aimed to investigate the antibacterial activity of three endodontic sealers, AH Plus, iRoot SP, and EndoSeal MTA, against four planktonic bacteria species. The antibacterial activity of the three endodontic sealers was assessed using a modified direct contact test. Bacteria suspension of...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/6/2012 |
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| author | Siew Thong Mak Xin Fang Leong In Meei Tew Endang Kumolosasi Lishen Wong |
| author_facet | Siew Thong Mak Xin Fang Leong In Meei Tew Endang Kumolosasi Lishen Wong |
| author_sort | Siew Thong Mak |
| collection | DOAJ |
| description | This study aimed to investigate the antibacterial activity of three endodontic sealers, AH Plus, iRoot SP, and EndoSeal MTA, against four planktonic bacteria species. The antibacterial activity of the three endodontic sealers was assessed using a modified direct contact test. Bacteria suspension of <i>Actinomycoses viscosus, Enterococcus faecalis</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus mutans</i> were left in contact with the sealers that were pre-set or set for 1, 3, 7, and l4 days for an hour. Freshly mixed AH Plus and EndoSeal MTA were highly effective against all four tested bacteria as no surviving bacteria were recovered after treatment. Meanwhile, freshly mixed iRoot SP was not able to kill all bacteria, regardless of the species, demonstrating a weak antibacterial effect. After 24 h, AH Plus lost its antibacterial activity. EndoSeal MTA showed a strong and extended bactericidal effect against <i>S. aureus</i> and <i>S. mutans</i> for 3 days and <i>A. viscosus</i> for 7 days. In conclusion, fresh AH Plus and EndoSeal MTA exhibited a potent effect against all four bacteria species. EndoSeal MTA remained effective after setting when tested against <i>A. viscosus, S. aureus</i>, and <i>S. mutans</i>. Among all tested sealers, iRoot SP demonstrates the weakest antibacterial activity. |
| first_indexed | 2024-03-09T13:31:14Z |
| format | Article |
| id | doaj.art-7c17f02964a64cbfab480f9b405e4b9e |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T13:31:14Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-7c17f02964a64cbfab480f9b405e4b9e2023-11-30T21:17:27ZengMDPI AGMaterials1996-19442022-03-01156201210.3390/ma15062012<i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic BacteriaSiew Thong Mak0Xin Fang Leong1In Meei Tew2Endang Kumolosasi3Lishen Wong4Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaDepartment of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaDepartment of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaDrug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaDepartment of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaThis study aimed to investigate the antibacterial activity of three endodontic sealers, AH Plus, iRoot SP, and EndoSeal MTA, against four planktonic bacteria species. The antibacterial activity of the three endodontic sealers was assessed using a modified direct contact test. Bacteria suspension of <i>Actinomycoses viscosus, Enterococcus faecalis</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus mutans</i> were left in contact with the sealers that were pre-set or set for 1, 3, 7, and l4 days for an hour. Freshly mixed AH Plus and EndoSeal MTA were highly effective against all four tested bacteria as no surviving bacteria were recovered after treatment. Meanwhile, freshly mixed iRoot SP was not able to kill all bacteria, regardless of the species, demonstrating a weak antibacterial effect. After 24 h, AH Plus lost its antibacterial activity. EndoSeal MTA showed a strong and extended bactericidal effect against <i>S. aureus</i> and <i>S. mutans</i> for 3 days and <i>A. viscosus</i> for 7 days. In conclusion, fresh AH Plus and EndoSeal MTA exhibited a potent effect against all four bacteria species. EndoSeal MTA remained effective after setting when tested against <i>A. viscosus, S. aureus</i>, and <i>S. mutans</i>. Among all tested sealers, iRoot SP demonstrates the weakest antibacterial activity.https://www.mdpi.com/1996-1944/15/6/2012antibacterialmodified direct contact testendodontic sealerbacteriaplanktonic |
| spellingShingle | Siew Thong Mak Xin Fang Leong In Meei Tew Endang Kumolosasi Lishen Wong <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria Materials antibacterial modified direct contact test endodontic sealer bacteria planktonic |
| title | <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria |
| title_full | <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria |
| title_fullStr | <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria |
| title_full_unstemmed | <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria |
| title_short | <i>In Vitro</i> Evaluation of the Antibacterial Activity of EndoSeal MTA, iRoot SP, and AH Plus against Planktonic Bacteria |
| title_sort | i in vitro i evaluation of the antibacterial activity of endoseal mta iroot sp and ah plus against planktonic bacteria |
| topic | antibacterial modified direct contact test endodontic sealer bacteria planktonic |
| url | https://www.mdpi.com/1996-1944/15/6/2012 |
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