Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture
Summary: The three-dimensional architecture of the genome plays an essential role in establishing and maintaining cell identity. However, the magnitude and temporal kinetics of changes in chromatin structure that arise during cell differentiation remain poorly understood. Here, we leverage a murine...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220307756 |
| _version_ | 1818047667633127424 |
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| author | Andrea A. Perreault Jonathan D. Brown Bryan J. Venters |
| author_facet | Andrea A. Perreault Jonathan D. Brown Bryan J. Venters |
| author_sort | Andrea A. Perreault |
| collection | DOAJ |
| description | Summary: The three-dimensional architecture of the genome plays an essential role in establishing and maintaining cell identity. However, the magnitude and temporal kinetics of changes in chromatin structure that arise during cell differentiation remain poorly understood. Here, we leverage a murine model of erythropoiesis to study the relationship between chromatin conformation, the epigenome, and transcription in erythroid cells. We discover that acute transcriptional responses induced by erythropoietin (EPO), the hormone necessary for erythroid differentiation, occur within an invariant chromatin topology. Within this pre-established landscape, Yin Yang 1 (YY1) occupancy dynamically redistributes to sites in proximity of EPO-regulated genes. Using HiChIP, we identify chromatin contacts mediated by H3K27ac and YY1 that are enriched for enhancer-promoter interactions of EPO-responsive genes. Taken together, these data are consistent with an emerging model that rapid, signal-dependent transcription occurs in the context of a pre-established chromatin architecture. |
| first_indexed | 2024-12-10T10:09:26Z |
| format | Article |
| id | doaj.art-7c1e077f977245769d79eb4e0928056f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-10T10:09:26Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-7c1e077f977245769d79eb4e0928056f2022-12-22T01:53:10ZengElsevieriScience2589-00422020-10-012310101583Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin ArchitectureAndrea A. Perreault0Jonathan D. Brown1Bryan J. Venters2Chemical and Physical Biology Program, Vanderbilt University, Nashville, TN 37232, USA; Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Corresponding authorDivision of Cardiovascular Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USAMolecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Corresponding authorSummary: The three-dimensional architecture of the genome plays an essential role in establishing and maintaining cell identity. However, the magnitude and temporal kinetics of changes in chromatin structure that arise during cell differentiation remain poorly understood. Here, we leverage a murine model of erythropoiesis to study the relationship between chromatin conformation, the epigenome, and transcription in erythroid cells. We discover that acute transcriptional responses induced by erythropoietin (EPO), the hormone necessary for erythroid differentiation, occur within an invariant chromatin topology. Within this pre-established landscape, Yin Yang 1 (YY1) occupancy dynamically redistributes to sites in proximity of EPO-regulated genes. Using HiChIP, we identify chromatin contacts mediated by H3K27ac and YY1 that are enriched for enhancer-promoter interactions of EPO-responsive genes. Taken together, these data are consistent with an emerging model that rapid, signal-dependent transcription occurs in the context of a pre-established chromatin architecture.http://www.sciencedirect.com/science/article/pii/S2589004220307756Molecular BiologyDevelopmental BiologyGenomics |
| spellingShingle | Andrea A. Perreault Jonathan D. Brown Bryan J. Venters Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture iScience Molecular Biology Developmental Biology Genomics |
| title | Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture |
| title_full | Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture |
| title_fullStr | Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture |
| title_full_unstemmed | Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture |
| title_short | Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture |
| title_sort | erythropoietin regulates transcription and yy1 dynamics in a pre established chromatin architecture |
| topic | Molecular Biology Developmental Biology Genomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220307756 |
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