The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles
Long-term and short-term exposure to carbon nanoparticles (CNPs) can affect fetal development and subsequent adverse outcomes including preterm delivery, intrauterine growth restriction, low birth weight, increased health risk linked to cardiovascular, respiratory and nervous systems in adulthood. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322002573 |
| _version_ | 1819174711341350912 |
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| author | Leilei Shi Yaru Qian Qian Shen Yanan He Yuqiao Jia Feifei Wang |
| author_facet | Leilei Shi Yaru Qian Qian Shen Yanan He Yuqiao Jia Feifei Wang |
| author_sort | Leilei Shi |
| collection | DOAJ |
| description | Long-term and short-term exposure to carbon nanoparticles (CNPs) can affect fetal development and subsequent adverse outcomes including preterm delivery, intrauterine growth restriction, low birth weight, increased health risk linked to cardiovascular, respiratory and nervous systems in adulthood. The adverse developmental outcomes of CNPs were well known, but the underlying mechanisms remain unresolved. In this study, zebrafish embryos were treated with CNPs of 50,100,200 μg/mL and the toxic effects were observed. Using the RNA-seq analysis approach, we examined the effects of CNPs (200 μg/mL) on gene expression in zebrafish embryos exposed from 4 to 96 h-post-fertilization (hpf). We observed that CNPs-treated embryos exhibited increased malformations and decreased hatching. A total of 236 differentially expressed genes were detected by transcriptome analyses, which were associated with phototransduction, amino acid metabolism, steroid and steroid hormone biosynthesis. Transcriptome results were verified by real-time fluorescence quantitative PCR (RT-qPCR). Our results indicated that CNPs exposure was most likely to lead to differential gene changes in steroid and hormone biosynthesis pathways, thus inducing developmental toxicity such as delayed incubation of zebrafish embryos, increased malformation rate and multiple malformation phenotypes. |
| first_indexed | 2024-12-22T20:43:19Z |
| format | Article |
| id | doaj.art-7c1ed1c4246e49fab8d7f2af311aafec |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-22T20:43:19Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-7c1ed1c4246e49fab8d7f2af311aafec2022-12-21T18:13:17ZengElsevierEcotoxicology and Environmental Safety0147-65132022-04-01234113417The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticlesLeilei Shi0Yaru Qian1Qian Shen2Yanan He3Yuqiao Jia4Feifei Wang5School of Public Health, Baotou Medical College, Baotou, Inner Mongolia 014040, PR ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 10012, PR ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 10012, PR ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 10012, PR ChinaSchool of Public Health, Baotou Medical College, Baotou, Inner Mongolia 014040, PR China; Corresponding author.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 10012, PR China; Correspondence to: Chinese Research Academy of Environmental Sciences, Beijing 100012, China.Long-term and short-term exposure to carbon nanoparticles (CNPs) can affect fetal development and subsequent adverse outcomes including preterm delivery, intrauterine growth restriction, low birth weight, increased health risk linked to cardiovascular, respiratory and nervous systems in adulthood. The adverse developmental outcomes of CNPs were well known, but the underlying mechanisms remain unresolved. In this study, zebrafish embryos were treated with CNPs of 50,100,200 μg/mL and the toxic effects were observed. Using the RNA-seq analysis approach, we examined the effects of CNPs (200 μg/mL) on gene expression in zebrafish embryos exposed from 4 to 96 h-post-fertilization (hpf). We observed that CNPs-treated embryos exhibited increased malformations and decreased hatching. A total of 236 differentially expressed genes were detected by transcriptome analyses, which were associated with phototransduction, amino acid metabolism, steroid and steroid hormone biosynthesis. Transcriptome results were verified by real-time fluorescence quantitative PCR (RT-qPCR). Our results indicated that CNPs exposure was most likely to lead to differential gene changes in steroid and hormone biosynthesis pathways, thus inducing developmental toxicity such as delayed incubation of zebrafish embryos, increased malformation rate and multiple malformation phenotypes.http://www.sciencedirect.com/science/article/pii/S0147651322002573Carbon nanoparticlesZebrafish embryosTranscriptomic analysisDevelopmental toxicityMalformation phenotype |
| spellingShingle | Leilei Shi Yaru Qian Qian Shen Yanan He Yuqiao Jia Feifei Wang The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles Ecotoxicology and Environmental Safety Carbon nanoparticles Zebrafish embryos Transcriptomic analysis Developmental toxicity Malformation phenotype |
| title | The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles |
| title_full | The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles |
| title_fullStr | The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles |
| title_full_unstemmed | The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles |
| title_short | The developmental toxicity and transcriptome analyses of zebrafish (Danio rerio) embryos exposed to carbon nanoparticles |
| title_sort | developmental toxicity and transcriptome analyses of zebrafish danio rerio embryos exposed to carbon nanoparticles |
| topic | Carbon nanoparticles Zebrafish embryos Transcriptomic analysis Developmental toxicity Malformation phenotype |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322002573 |
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