Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry
Resveratrol is an antitoxin secreted by plants such as Polygonum cuspidatum Sieb. et Zucc and Vitis vinifera L. when they are attacked by pathogens. In the present study, three methods were used to prepare biological samples, and then an efficient strategy based on ultra-high-performance liquid chro...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535220302598 |
| _version_ | 1818188663224270848 |
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| author | Zihan Liu Shaoping Wang Fan Dong Ying Lin Haoran Li Lei Shi Zhibin Wang Jiayu Zhang |
| author_facet | Zihan Liu Shaoping Wang Fan Dong Ying Lin Haoran Li Lei Shi Zhibin Wang Jiayu Zhang |
| author_sort | Zihan Liu |
| collection | DOAJ |
| description | Resveratrol is an antitoxin secreted by plants such as Polygonum cuspidatum Sieb. et Zucc and Vitis vinifera L. when they are attacked by pathogens. In the present study, three methods were used to prepare biological samples, and then an efficient strategy based on ultra-high-performance liquid chromatography-linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed to screen and identify resveratrol metabolites in rat urine, plasma and faeces. As a result, a total of 56 resveratrol metabolites were screened and characterized. Among them, 39 metabolites were found in rat urine, while 6 and 16 metabolites were characterized from rat plasma and faeces, respectively. In addition, 56, 12 and 15 metabolic products were screened by solid phase extraction (method I), methanol precipitation (method II) and acetonitrile precipitation (method III), respectively, indicating that method I could be adopted as the most acceptable method. The results also demonstrated that resveratrol mainly underwent glucuronidation, glucosylation, sulfation, hydroxylation, dehydrogenation, hydrogenation, methylation and their composite reactions. Moreover, these metabolic reactions occurred to form a possible metabolic network that is similar to a triangular pyramid model. In summary, this research provides an idea for the further study of drug metabolism. |
| first_indexed | 2024-12-11T23:30:30Z |
| format | Article |
| id | doaj.art-7c2082e6cb6d4c9eaddbc1ebbaf3196c |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-11T23:30:30Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-7c2082e6cb6d4c9eaddbc1ebbaf3196c2022-12-22T00:46:04ZengElsevierArabian Journal of Chemistry1878-53522020-09-0113970557065Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometryZihan Liu0Shaoping Wang1Fan Dong2Ying Lin3Haoran Li4Lei Shi5Zhibin Wang6Jiayu Zhang7School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China; School of Pharmacy, BIN ZHOU Medical University, 264003, ChinaSchool of Pharmacy, BIN ZHOU Medical University, 264003, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China; School of Pharmacy, BIN ZHOU Medical University, 264003, ChinaSchool of Pharmacy, BIN ZHOU Medical University, 264003, ChinaShandong University of Traditional Chinese Medicine, Jinan 250300, ChinaShandong University of Traditional Chinese Medicine, Jinan 250300, ChinaTongrentang Research Institute, Beijing 100079, China; Corresponding authors.School of Pharmacy, BIN ZHOU Medical University, 264003, China; Corresponding authors.Resveratrol is an antitoxin secreted by plants such as Polygonum cuspidatum Sieb. et Zucc and Vitis vinifera L. when they are attacked by pathogens. In the present study, three methods were used to prepare biological samples, and then an efficient strategy based on ultra-high-performance liquid chromatography-linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed to screen and identify resveratrol metabolites in rat urine, plasma and faeces. As a result, a total of 56 resveratrol metabolites were screened and characterized. Among them, 39 metabolites were found in rat urine, while 6 and 16 metabolites were characterized from rat plasma and faeces, respectively. In addition, 56, 12 and 15 metabolic products were screened by solid phase extraction (method I), methanol precipitation (method II) and acetonitrile precipitation (method III), respectively, indicating that method I could be adopted as the most acceptable method. The results also demonstrated that resveratrol mainly underwent glucuronidation, glucosylation, sulfation, hydroxylation, dehydrogenation, hydrogenation, methylation and their composite reactions. Moreover, these metabolic reactions occurred to form a possible metabolic network that is similar to a triangular pyramid model. In summary, this research provides an idea for the further study of drug metabolism.http://www.sciencedirect.com/science/article/pii/S1878535220302598ResveratrolMetabliteUHPLC-LTQ-Orbitrap MSMetabolic network |
| spellingShingle | Zihan Liu Shaoping Wang Fan Dong Ying Lin Haoran Li Lei Shi Zhibin Wang Jiayu Zhang Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry Arabian Journal of Chemistry Resveratrol Metablite UHPLC-LTQ-Orbitrap MS Metabolic network |
| title | Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| title_full | Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| title_fullStr | Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| title_full_unstemmed | Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| title_short | Comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| title_sort | comprehensive analysis of resveratrol metabolites in rats using ultra high performance liquid chromatography coupled with high resolution mass spectrometry |
| topic | Resveratrol Metablite UHPLC-LTQ-Orbitrap MS Metabolic network |
| url | http://www.sciencedirect.com/science/article/pii/S1878535220302598 |
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