Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
The nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We foun...
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| Format: | Article |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.648331/full |
| _version_ | 1818429934265171968 |
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| author | Ting Wang Ting Wang Jie Na |
| author_facet | Ting Wang Ting Wang Jie Na |
| author_sort | Ting Wang |
| collection | DOAJ |
| description | The nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We found that FBL–GFP displays an uneven and cloudy localization in the nucleolus of non-surrounded nucleolus (NSN) oocytes, while it distributes evenly and to a few bright dots in the surrounded nucleolus (SN) oocytes. Accordingly, NSN oocytes showed active nascent RNA transcription, while the SN group was transcriptionally quiescent. NSN geminal vesicles also contained more DNA damage marker γH2AX foci. Based on different FBL–GFP patterns in live oocytes, the ones with superior meiotic maturation potential can be identified. Global transcriptome profiling revealed a significant difference in single SN and NSN oocytes. Thus, FBL–GFP can serve as a marker for nucleolus activity, which also correlates with transcription activity and the quality of oocytes. |
| first_indexed | 2024-12-14T15:25:24Z |
| format | Article |
| id | doaj.art-7c21021c642b401e992eac2ecd3a415d |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T15:25:24Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-7c21021c642b401e992eac2ecd3a415d2022-12-21T22:56:02ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-04-01910.3389/fcell.2021.648331648331Fibrillarin-GFP Facilitates the Identification of Meiotic Competent OocytesTing Wang0Ting Wang1Jie Na2Center for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing, ChinaTsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, ChinaCenter for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing, ChinaThe nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We found that FBL–GFP displays an uneven and cloudy localization in the nucleolus of non-surrounded nucleolus (NSN) oocytes, while it distributes evenly and to a few bright dots in the surrounded nucleolus (SN) oocytes. Accordingly, NSN oocytes showed active nascent RNA transcription, while the SN group was transcriptionally quiescent. NSN geminal vesicles also contained more DNA damage marker γH2AX foci. Based on different FBL–GFP patterns in live oocytes, the ones with superior meiotic maturation potential can be identified. Global transcriptome profiling revealed a significant difference in single SN and NSN oocytes. Thus, FBL–GFP can serve as a marker for nucleolus activity, which also correlates with transcription activity and the quality of oocytes.https://www.frontiersin.org/articles/10.3389/fcell.2021.648331/fullnucleolusfibrillarinmeiosisoocyte maturationtranscriptome analysis |
| spellingShingle | Ting Wang Ting Wang Jie Na Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes Frontiers in Cell and Developmental Biology nucleolus fibrillarin meiosis oocyte maturation transcriptome analysis |
| title | Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes |
| title_full | Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes |
| title_fullStr | Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes |
| title_full_unstemmed | Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes |
| title_short | Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes |
| title_sort | fibrillarin gfp facilitates the identification of meiotic competent oocytes |
| topic | nucleolus fibrillarin meiosis oocyte maturation transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.648331/full |
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