Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets
(1) Background: Piglet diarrhea is one of the most serious diseases in pigs and has brought great economic losses to the pig industry. Alteration of the gut microbiota is an important factor in the etiology of piglet diarrhea. Therefore, this study aimed to analyze the differences in the gut microbi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1166 |
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| author | Xianrui Zheng Ke Nie Yiliang Xu Huibin Zhang Fan Xie Liming Xu Zhiyong Zhang Yueyun Ding Zongjun Yin Xiaodong Zhang |
| author_facet | Xianrui Zheng Ke Nie Yiliang Xu Huibin Zhang Fan Xie Liming Xu Zhiyong Zhang Yueyun Ding Zongjun Yin Xiaodong Zhang |
| author_sort | Xianrui Zheng |
| collection | DOAJ |
| description | (1) Background: Piglet diarrhea is one of the most serious diseases in pigs and has brought great economic losses to the pig industry. Alteration of the gut microbiota is an important factor in the etiology of piglet diarrhea. Therefore, this study aimed to analyze the differences in the gut microbial structures and fecal metabolic profile between post-weaning diarrhea and healthy Chinese Wannan Black pigs. (2) Methods: An integrated approach of 16S rRNA gene sequencing combined with LC/MS-based metabolomics was employed in this study. (3) Results: We found an increase in the relative abundance of the bacterial genus <i>Campylobacter</i> and a decrease in phylum Bacteroidetes and the species <i>Streptococcus gallolyticus</i> subsp. <i>macedonicus. (S. macedonicus)</i> in piglet diarrhea. Meanwhile, obvious changes in the fecal metabolic profile of diarrheic piglets were also detected, particularly higher levels of polyamines (spermine and spermidine). Moreover, there were substantial associations between the disturbed gut microbiota and the altered fecal metabolites, especially a strong positive relationship between spermidine and <i>Campylobacter</i>. (4) Conclusions: These observations may provide novel insights into potential etiologies related to post-weaning diarrhea and further enhance our understanding of the role of gut microbiota in host homeostasis and in modulating gut microbial structure. |
| first_indexed | 2024-03-11T02:26:26Z |
| format | Article |
| id | doaj.art-7c26003a0c0a4f4cb12699ed6b7997b7 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:26:26Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-7c26003a0c0a4f4cb12699ed6b7997b72023-11-18T10:33:43ZengMDPI AGGenes2073-44252023-05-01146116610.3390/genes14061166Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic PigletsXianrui Zheng0Ke Nie1Yiliang Xu2Huibin Zhang3Fan Xie4Liming Xu5Zhiyong Zhang6Yueyun Ding7Zongjun Yin8Xiaodong Zhang9College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaKey Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China(1) Background: Piglet diarrhea is one of the most serious diseases in pigs and has brought great economic losses to the pig industry. Alteration of the gut microbiota is an important factor in the etiology of piglet diarrhea. Therefore, this study aimed to analyze the differences in the gut microbial structures and fecal metabolic profile between post-weaning diarrhea and healthy Chinese Wannan Black pigs. (2) Methods: An integrated approach of 16S rRNA gene sequencing combined with LC/MS-based metabolomics was employed in this study. (3) Results: We found an increase in the relative abundance of the bacterial genus <i>Campylobacter</i> and a decrease in phylum Bacteroidetes and the species <i>Streptococcus gallolyticus</i> subsp. <i>macedonicus. (S. macedonicus)</i> in piglet diarrhea. Meanwhile, obvious changes in the fecal metabolic profile of diarrheic piglets were also detected, particularly higher levels of polyamines (spermine and spermidine). Moreover, there were substantial associations between the disturbed gut microbiota and the altered fecal metabolites, especially a strong positive relationship between spermidine and <i>Campylobacter</i>. (4) Conclusions: These observations may provide novel insights into potential etiologies related to post-weaning diarrhea and further enhance our understanding of the role of gut microbiota in host homeostasis and in modulating gut microbial structure.https://www.mdpi.com/2073-4425/14/6/1166weaned pigletsdiarrheagut microbiotafecal metabolites |
| spellingShingle | Xianrui Zheng Ke Nie Yiliang Xu Huibin Zhang Fan Xie Liming Xu Zhiyong Zhang Yueyun Ding Zongjun Yin Xiaodong Zhang Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets Genes weaned piglets diarrhea gut microbiota fecal metabolites |
| title | Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets |
| title_full | Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets |
| title_fullStr | Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets |
| title_full_unstemmed | Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets |
| title_short | Fecal Microbial Structure and Metabolic Profile in Post-Weaning Diarrheic Piglets |
| title_sort | fecal microbial structure and metabolic profile in post weaning diarrheic piglets |
| topic | weaned piglets diarrhea gut microbiota fecal metabolites |
| url | https://www.mdpi.com/2073-4425/14/6/1166 |
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