The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations
The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of sur...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5783 |
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| author | Martin Schicht Kamila Riedlová Mercedes Kukulka Wenyue Li Aurelius Scheer Fabian Garreis Christina Jacobi Friedrich Paulsen Lukasz Cwiklik Lars Bräuer |
| author_facet | Martin Schicht Kamila Riedlová Mercedes Kukulka Wenyue Li Aurelius Scheer Fabian Garreis Christina Jacobi Friedrich Paulsen Lukasz Cwiklik Lars Bräuer |
| author_sort | Martin Schicht |
| collection | DOAJ |
| description | The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. |
| first_indexed | 2024-03-10T03:42:34Z |
| format | Article |
| id | doaj.art-7c26f1cdaa7a48b99c776417555151f6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:42:34Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7c26f1cdaa7a48b99c776417555151f62023-11-23T11:28:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310578310.3390/ijms23105783The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental ObservationsMartin Schicht0Kamila Riedlová1Mercedes Kukulka2Wenyue Li3Aurelius Scheer4Fabian Garreis5Christina Jacobi6Friedrich Paulsen7Lukasz Cwiklik8Lars Bräuer9Institute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, 182 00 Prague, Czech RepublicJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, 182 00 Prague, Czech RepublicInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyOphthalmology Private Practice, 17, 81034 Nuremberg, GermanyInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, 182 00 Prague, Czech RepublicInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, GermanyThe ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film.https://www.mdpi.com/1422-0067/23/10/5783tear filmsurfactant proteinsurface tensionocular surfacedry eye |
| spellingShingle | Martin Schicht Kamila Riedlová Mercedes Kukulka Wenyue Li Aurelius Scheer Fabian Garreis Christina Jacobi Friedrich Paulsen Lukasz Cwiklik Lars Bräuer The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations International Journal of Molecular Sciences tear film surfactant protein surface tension ocular surface dry eye |
| title | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
| title_full | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
| title_fullStr | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
| title_full_unstemmed | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
| title_short | The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations |
| title_sort | potential role of sp g as surface tension regulator in tear film from molecular simulations to experimental observations |
| topic | tear film surfactant protein surface tension ocular surface dry eye |
| url | https://www.mdpi.com/1422-0067/23/10/5783 |
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