Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids
Abstract Photodecarboxylase from Chlorella variabillis (CvFAP) is one of the three known light‐activated enzymes that catalyzes the decarboxylation of fatty acids into the corresponding C1‐shortened alkanes. Although the substrate scope of CvFAP has been altered by protein engineering and decoy mole...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2021-05-01
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| Series: | ChemistryOpen |
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| Online Access: | https://doi.org/10.1002/open.202100039 |
| _version_ | 1818663353135923200 |
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| author | Yong‐Yi Zeng Prof. Dr. Lan Liu Dr. Bi‐Shuang Chen Prof. Dr. Wuyuan Zhang |
| author_facet | Yong‐Yi Zeng Prof. Dr. Lan Liu Dr. Bi‐Shuang Chen Prof. Dr. Wuyuan Zhang |
| author_sort | Yong‐Yi Zeng |
| collection | DOAJ |
| description | Abstract Photodecarboxylase from Chlorella variabillis (CvFAP) is one of the three known light‐activated enzymes that catalyzes the decarboxylation of fatty acids into the corresponding C1‐shortened alkanes. Although the substrate scope of CvFAP has been altered by protein engineering and decoy molecules, it is still limited to mono‐fatty acids. Our studies demonstrate for the first time that long chain dicarboxylic acids can be converted by CvFAP. Notably, the conversion of dicarboxylic acids to alkanes still represents a chemically very challenging reaction. Herein, the light‐driven enzymatic decarboxylation of dicarboxylic acids to the corresponding (C2‐shortened) alkanes using CvFAP is described. A series of dicarboxylic acids is decarboxylated into alkanes in good yields by means of this approach, even for the preparative scales. Reaction pathway studies show that mono‐fatty acids are formed as the intermediate products before the final release of C2‐shortened alkanes. In addition, the thermostability, storage stability, and recyclability of CvFAP for decarboxylation of dicarboxylic acids are well evaluated. These results represent an advancement over the current state‐of‐the‐art. |
| first_indexed | 2024-12-17T05:15:30Z |
| format | Article |
| id | doaj.art-7c2845a3c64d45318a5bea480ef81340 |
| institution | Directory Open Access Journal |
| issn | 2191-1363 |
| language | English |
| last_indexed | 2024-12-17T05:15:30Z |
| publishDate | 2021-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj.art-7c2845a3c64d45318a5bea480ef813402022-12-21T22:02:07ZengWiley-VCHChemistryOpen2191-13632021-05-0110555355910.1002/open.202100039Light‐Driven Enzymatic Decarboxylation of Dicarboxylic AcidsYong‐Yi Zeng0Prof. Dr. Lan Liu1Dr. Bi‐Shuang Chen2Prof. Dr. Wuyuan Zhang3School of Marine Sciences Sun Yat-Sen University Zhuhai 519082 P. R. ChinaSchool of Marine Sciences Sun Yat-Sen University Zhuhai 519082 P. R. ChinaSchool of Marine Sciences Sun Yat-Sen University Zhuhai 519082 P. R. ChinaTianjin Institute of Industrial Biotechnology Chinese Academy of Sciences West 7th Avenue Tianjin 300308 P. R. ChinaAbstract Photodecarboxylase from Chlorella variabillis (CvFAP) is one of the three known light‐activated enzymes that catalyzes the decarboxylation of fatty acids into the corresponding C1‐shortened alkanes. Although the substrate scope of CvFAP has been altered by protein engineering and decoy molecules, it is still limited to mono‐fatty acids. Our studies demonstrate for the first time that long chain dicarboxylic acids can be converted by CvFAP. Notably, the conversion of dicarboxylic acids to alkanes still represents a chemically very challenging reaction. Herein, the light‐driven enzymatic decarboxylation of dicarboxylic acids to the corresponding (C2‐shortened) alkanes using CvFAP is described. A series of dicarboxylic acids is decarboxylated into alkanes in good yields by means of this approach, even for the preparative scales. Reaction pathway studies show that mono‐fatty acids are formed as the intermediate products before the final release of C2‐shortened alkanes. In addition, the thermostability, storage stability, and recyclability of CvFAP for decarboxylation of dicarboxylic acids are well evaluated. These results represent an advancement over the current state‐of‐the‐art.https://doi.org/10.1002/open.202100039photobiocatalysisdecarboxylationdicarboxylic acidsalkanesbiodegradable plastic |
| spellingShingle | Yong‐Yi Zeng Prof. Dr. Lan Liu Dr. Bi‐Shuang Chen Prof. Dr. Wuyuan Zhang Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids ChemistryOpen photobiocatalysis decarboxylation dicarboxylic acids alkanes biodegradable plastic |
| title | Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids |
| title_full | Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids |
| title_fullStr | Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids |
| title_full_unstemmed | Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids |
| title_short | Light‐Driven Enzymatic Decarboxylation of Dicarboxylic Acids |
| title_sort | light driven enzymatic decarboxylation of dicarboxylic acids |
| topic | photobiocatalysis decarboxylation dicarboxylic acids alkanes biodegradable plastic |
| url | https://doi.org/10.1002/open.202100039 |
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