Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
Advances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as bio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/1/139 |
| _version_ | 1827664055290036224 |
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| author | Premanshu Kumar Singh Aarti Patel Anastasia Kaffenes Catherine Hord Delaney Kesterson Shaurya Prakash |
| author_facet | Premanshu Kumar Singh Aarti Patel Anastasia Kaffenes Catherine Hord Delaney Kesterson Shaurya Prakash |
| author_sort | Premanshu Kumar Singh |
| collection | DOAJ |
| description | Advances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as biomarker cargo holders have generated significant interest. However, the isolation, purification, and subsequent analysis of these extracellular vesicles remain a challenge. Technological advances driven by microfluidics-enabled devices have made the challenges for isolation of extracellular vesicles an emerging area of research with significant possibilities for use in clinical settings enabling point-of-care diagnostics for cancer. In this article, we present a tutorial review of the existing microfluidic technologies for cancer diagnostics with a focus on extracellular vesicle isolation methods. |
| first_indexed | 2024-03-10T00:54:52Z |
| format | Article |
| id | doaj.art-7c2ba45d1a3e4b1e85ccc7f0822bb3d5 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T00:54:52Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-7c2ba45d1a3e4b1e85ccc7f0822bb3d52023-11-23T14:45:35ZengMDPI AGMicromachines2072-666X2022-01-0113113910.3390/mi13010139Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer DiagnosticsPremanshu Kumar Singh0Aarti Patel1Anastasia Kaffenes2Catherine Hord3Delaney Kesterson4Shaurya Prakash5Department of Mechanical and Aerospace Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USADepartment of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USADepartment of Neuroscience, College of Arts and Sciences and College of Medicine, The Ohio State University, Columbus, OH 43210, USACenter for Life Sciences Education, The Ohio State University, Columbus, OH 43210, USACenter for Life Sciences Education, The Ohio State University, Columbus, OH 43210, USADepartment of Mechanical and Aerospace Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USAAdvances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as biomarker cargo holders have generated significant interest. However, the isolation, purification, and subsequent analysis of these extracellular vesicles remain a challenge. Technological advances driven by microfluidics-enabled devices have made the challenges for isolation of extracellular vesicles an emerging area of research with significant possibilities for use in clinical settings enabling point-of-care diagnostics for cancer. In this article, we present a tutorial review of the existing microfluidic technologies for cancer diagnostics with a focus on extracellular vesicle isolation methods.https://www.mdpi.com/2072-666X/13/1/139extracellular vesiclesexosomemicrofluidicscancer diagnosis |
| spellingShingle | Premanshu Kumar Singh Aarti Patel Anastasia Kaffenes Catherine Hord Delaney Kesterson Shaurya Prakash Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics Micromachines extracellular vesicles exosome microfluidics cancer diagnosis |
| title | Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics |
| title_full | Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics |
| title_fullStr | Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics |
| title_full_unstemmed | Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics |
| title_short | Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics |
| title_sort | microfluidic approaches and methods enabling extracellular vesicle isolation for cancer diagnostics |
| topic | extracellular vesicles exosome microfluidics cancer diagnosis |
| url | https://www.mdpi.com/2072-666X/13/1/139 |
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