Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples
Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157521000055 |
| _version_ | 1818656543553355776 |
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| author | Lu Zeng Lingling Guo Zhongxing Wang Xinxin Xu Hongliu Ding Shanshan Song Liguang Xu Hua Kuang Chuanlai Xu |
| author_facet | Lu Zeng Lingling Guo Zhongxing Wang Xinxin Xu Hongliu Ding Shanshan Song Liguang Xu Hua Kuang Chuanlai Xu |
| author_sort | Lu Zeng |
| collection | DOAJ |
| description | Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 104 CFU/mL and the linear range of detection was 3.13 × 104-1.0 × 106 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting P. aeruginosa (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of P. aeruginosa (CICC 10419) after 8 h enrichment. |
| first_indexed | 2024-12-17T03:27:15Z |
| format | Article |
| id | doaj.art-7c2d3f7d916240e7a265ed0fdc38a2a6 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-12-17T03:27:15Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-7c2d3f7d916240e7a265ed0fdc38a2a62022-12-21T22:05:21ZengElsevierFood Chemistry: X2590-15752021-03-019100117Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samplesLu Zeng0Lingling Guo1Zhongxing Wang2Xinxin Xu3Hongliu Ding4Shanshan Song5Liguang Xu6Hua Kuang7Chuanlai Xu8State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaSuzhou Product Quality Supervision Inspection, 1368 Wuzhong Avenue, Suzhou 215104, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; Corresponding authors at: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; Corresponding authors at: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China.Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 104 CFU/mL and the linear range of detection was 3.13 × 104-1.0 × 106 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting P. aeruginosa (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of P. aeruginosa (CICC 10419) after 8 h enrichment.http://www.sciencedirect.com/science/article/pii/S2590157521000055Immunochromatographic assayPseudomonas aeruginosaMonoclonal antibodies |
| spellingShingle | Lu Zeng Lingling Guo Zhongxing Wang Xinxin Xu Hongliu Ding Shanshan Song Liguang Xu Hua Kuang Chuanlai Xu Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples Food Chemistry: X Immunochromatographic assay Pseudomonas aeruginosa Monoclonal antibodies |
| title | Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples |
| title_full | Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples |
| title_fullStr | Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples |
| title_full_unstemmed | Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples |
| title_short | Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples |
| title_sort | gold nanoparticle based immunochromatographic assay for detection pseudomonas aeruginosa in water and food samples |
| topic | Immunochromatographic assay Pseudomonas aeruginosa Monoclonal antibodies |
| url | http://www.sciencedirect.com/science/article/pii/S2590157521000055 |
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