Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microp...
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Academia Brasileira de Ciências
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| Series: | Anais da Academia Brasileira de Ciências |
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| author | ISLA V.G.A. BASTOS TATIANE B. DE OLIVEIRA MARIA D. RODRIGUES GARDÊNIA C.G. MILITÃO TERESINHA G. DA SILVA IZABEL C.C. TURATTI NORBERTO P. LOPES SEBASTIÃO J. DE MELO |
| author_facet | ISLA V.G.A. BASTOS TATIANE B. DE OLIVEIRA MARIA D. RODRIGUES GARDÊNIA C.G. MILITÃO TERESINHA G. DA SILVA IZABEL C.C. TURATTI NORBERTO P. LOPES SEBASTIÃO J. DE MELO |
| author_sort | ISLA V.G.A. BASTOS |
| collection | DOAJ |
| description | ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth. |
| first_indexed | 2024-04-12T07:27:01Z |
| format | Article |
| id | doaj.art-7c2f9fdec2a44dbebb957f8c2a5bf797 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-12T07:27:01Z |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-7c2f9fdec2a44dbebb957f8c2a5bf7972022-12-22T03:42:09ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-2690892991100110.1590/0001-3765201720160586S0001-37652017000300991Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)ISLA V.G.A. BASTOSTATIANE B. DE OLIVEIRAMARIA D. RODRIGUESGARDÊNIA C.G. MILITÃOTERESINHA G. DA SILVAIZABEL C.C. TURATTINORBERTO P. LOPESSEBASTIÃO J. DE MELOABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991&lng=en&tlng=enCytotoxicityGas ChromatographyPhoradendron microphyllumPhoradendron mucronatum |
| spellingShingle | ISLA V.G.A. BASTOS TATIANE B. DE OLIVEIRA MARIA D. RODRIGUES GARDÊNIA C.G. MILITÃO TERESINHA G. DA SILVA IZABEL C.C. TURATTI NORBERTO P. LOPES SEBASTIÃO J. DE MELO Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) Anais da Academia Brasileira de Ciências Cytotoxicity Gas Chromatography Phoradendron microphyllum Phoradendron mucronatum |
| title | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) |
| title_full | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) |
| title_fullStr | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) |
| title_full_unstemmed | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) |
| title_short | Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) |
| title_sort | use of gc ms to identify chemical constituents and cytotoxic activity of the leaves of phoradendron mucronatum and phoradendron microphyllum viscaceae |
| topic | Cytotoxicity Gas Chromatography Phoradendron microphyllum Phoradendron mucronatum |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991&lng=en&tlng=en |
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