Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market
We set forth to assess the quality of an herbal medicine sold in Hong Kong called Qianliguang by employing a multi-methodological approach. The quality is set by its identity, chemical composition, and bioactivities, among others. Qianliguang (Senecionis scandentis Herba, Senecio scandens Buch.-Ham....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009707/?tool=EBI |
| _version_ | 1818022268747382784 |
|---|---|
| author | Hiu-Lam Ngai Xiao Yang Adrian Jun Chu Rachel Harper Alice B. J. E. Jacobsen David Tai-Wai Lau Ho-Yin Yu Hung-Kay Lee Pang-Chui Shaw |
| author_facet | Hiu-Lam Ngai Xiao Yang Adrian Jun Chu Rachel Harper Alice B. J. E. Jacobsen David Tai-Wai Lau Ho-Yin Yu Hung-Kay Lee Pang-Chui Shaw |
| author_sort | Hiu-Lam Ngai |
| collection | DOAJ |
| description | We set forth to assess the quality of an herbal medicine sold in Hong Kong called Qianliguang by employing a multi-methodological approach. The quality is set by its identity, chemical composition, and bioactivities, among others. Qianliguang (Senecionis scandentis Herba, Senecio scandens Buch.-Ham. ex D.Don) has known antibacterial properties. However, it is poisonous and overconsumption can result in liver damage. Eighteen Qianliguang samples were purchased from herbal shops at various districts in Hong Kong. Samples were first authenticated organoleptically. DNA barcoding at the psbA-trnH, ITS2, and rbcL loci was then conducted to confirm the species. HPLC-UV was performed to screen for the presence of the chemical compounds and to quantify the flavonoid hyperoside. UPLC-MS was used to quantify the amount of the toxic pyrrolizidine alkaloid (PA) adonifoline. Microdilution assay was performed to show the antibacterial effect on Streptococcus aureus and S. pneumoniae. Results showed that five samples were found to be substituted by species belonging to the genus Lespedeza; four samples were mixtures containing not only Qianliguang but also Achyranthes aspera L., Lonicera confusa DC., or Solanum nigrum L. HPLC-UV showed that only ten contained enough hyperoside to meet the standard requirement. In addition, nine samples had adonifoline that exceeded the toxicity standard requirement. In the microdilution assay, samples containing Qianliguang showed inhibition on S. aureus and S. pneumoniae, while among the five Lespedeza sp. samples the antibacterial effects on S. aureus were not detectable; only one sample showed inhibition to S. pneumoniae. Our study illustrated the necessity of using a multi-methodological approach for herbal medicine quality assessment. We also showed that Qianliguang samples in the Hong Kong market were either toxic or adulterated. It is therefore essential to improve the quality control of Qianliguang and probably other herbs in the herbal market. |
| first_indexed | 2024-04-14T08:30:01Z |
| format | Article |
| id | doaj.art-7c33ed0f240c44cd99a20b6d8da0d308 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:30:01Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7c33ed0f240c44cd99a20b6d8da0d3082022-12-22T02:03:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01174Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal marketHiu-Lam NgaiXiao YangAdrian Jun ChuRachel HarperAlice B. J. E. JacobsenDavid Tai-Wai LauHo-Yin YuHung-Kay LeePang-Chui ShawWe set forth to assess the quality of an herbal medicine sold in Hong Kong called Qianliguang by employing a multi-methodological approach. The quality is set by its identity, chemical composition, and bioactivities, among others. Qianliguang (Senecionis scandentis Herba, Senecio scandens Buch.-Ham. ex D.Don) has known antibacterial properties. However, it is poisonous and overconsumption can result in liver damage. Eighteen Qianliguang samples were purchased from herbal shops at various districts in Hong Kong. Samples were first authenticated organoleptically. DNA barcoding at the psbA-trnH, ITS2, and rbcL loci was then conducted to confirm the species. HPLC-UV was performed to screen for the presence of the chemical compounds and to quantify the flavonoid hyperoside. UPLC-MS was used to quantify the amount of the toxic pyrrolizidine alkaloid (PA) adonifoline. Microdilution assay was performed to show the antibacterial effect on Streptococcus aureus and S. pneumoniae. Results showed that five samples were found to be substituted by species belonging to the genus Lespedeza; four samples were mixtures containing not only Qianliguang but also Achyranthes aspera L., Lonicera confusa DC., or Solanum nigrum L. HPLC-UV showed that only ten contained enough hyperoside to meet the standard requirement. In addition, nine samples had adonifoline that exceeded the toxicity standard requirement. In the microdilution assay, samples containing Qianliguang showed inhibition on S. aureus and S. pneumoniae, while among the five Lespedeza sp. samples the antibacterial effects on S. aureus were not detectable; only one sample showed inhibition to S. pneumoniae. Our study illustrated the necessity of using a multi-methodological approach for herbal medicine quality assessment. We also showed that Qianliguang samples in the Hong Kong market were either toxic or adulterated. It is therefore essential to improve the quality control of Qianliguang and probably other herbs in the herbal market.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009707/?tool=EBI |
| spellingShingle | Hiu-Lam Ngai Xiao Yang Adrian Jun Chu Rachel Harper Alice B. J. E. Jacobsen David Tai-Wai Lau Ho-Yin Yu Hung-Kay Lee Pang-Chui Shaw Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market PLoS ONE |
| title | Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market |
| title_full | Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market |
| title_fullStr | Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market |
| title_full_unstemmed | Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market |
| title_short | Multi-methodological approach for the Quality assessment of Senecionis scandentis Herba (Qianliguang) in the herbal market |
| title_sort | multi methodological approach for the quality assessment of senecionis scandentis herba qianliguang in the herbal market |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009707/?tool=EBI |
| work_keys_str_mv | AT hiulamngai multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT xiaoyang multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT adrianjunchu multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT rachelharper multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT alicebjejacobsen multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT davidtaiwailau multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT hoyinyu multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT hungkaylee multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket AT pangchuishaw multimethodologicalapproachforthequalityassessmentofsenecionisscandentisherbaqianliguangintheherbalmarket |