Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle
This paper aims to describe the construction and operation of a respiration chamber of the head-box type for methane (CH<sub>4</sub>) measurements in bovines. The system consists of (1) a head box with a stainless steel frame and acrylic walls, floor, and ceiling; (2) a stainless steel f...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/2/227 |
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| author | Octavio Alonso Castelán Ortega Paulina Elizabeth Pedraza Beltrán Gloria Stefanny Hernández Pineda Mohammed Benaouda Manuel González Ronquillo Luisa T Molina Juan Carlos Ku Vera Hugo Daniel Montelongo Pérez María Fernanda Vázquez Carrillo |
| author_facet | Octavio Alonso Castelán Ortega Paulina Elizabeth Pedraza Beltrán Gloria Stefanny Hernández Pineda Mohammed Benaouda Manuel González Ronquillo Luisa T Molina Juan Carlos Ku Vera Hugo Daniel Montelongo Pérez María Fernanda Vázquez Carrillo |
| author_sort | Octavio Alonso Castelán Ortega |
| collection | DOAJ |
| description | This paper aims to describe the construction and operation of a respiration chamber of the head-box type for methane (CH<sub>4</sub>) measurements in bovines. The system consists of (1) a head box with a stainless steel frame and acrylic walls, floor, and ceiling; (2) a stainless steel feeder; (3) an automatic drinking water bowl; (4) a hood made from reinforced canvas; (5) an infrared (IR) CH<sub>4</sub> gas analyzer, a mass flow generator, a data-acquisition system; and (6) a steel metabolic box. Six assays were conducted to determine the pure CH<sub>4</sub> recovery rate of the whole system in order to validate it and comply with standards of chamber operation. The gravimetrical method was used for the recovery test and the recovery rate obtained was 1.04 ± 0.05. Once the system was calibrated, measurements of CH<sub>4</sub> were conducted using eight animals consisting of four Holstein cows with a live weight of 593.8 ± 51 kg and an average milk yield of 23.3 ± 1.8 kg d<sup>−1</sup> and four heifers with a live weight of 339 ± 28 kg. The CH<sub>4</sub> production values were 687 ± 123 and 248 ± 40 L CH<sub>4</sub> d<sup>−1</sup> for cows and heifers, respectively. The CH<sub>4</sub> yield was 19.7 ± 3.4 g and 17.1 ± 3.4 g CH<sub>4</sub> kg<sup>−1</sup> of dry matter consumed for cows and heifers, respectively. These results are consistent with those reported in the literature. |
| first_indexed | 2024-12-19T21:59:38Z |
| format | Article |
| id | doaj.art-7c364d4af12046d69c02326068082036 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-19T21:59:38Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7c364d4af12046d69c023260680820362022-12-21T20:04:11ZengMDPI AGAnimals2076-26152020-01-0110222710.3390/ani10020227ani10020227Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from CattleOctavio Alonso Castelán Ortega0Paulina Elizabeth Pedraza Beltrán1Gloria Stefanny Hernández Pineda2Mohammed Benaouda3Manuel González Ronquillo4Luisa T Molina5Juan Carlos Ku Vera6Hugo Daniel Montelongo Pérez7María Fernanda Vázquez Carrillo8Facultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoMolina Center for Strategic Studies in Energy and the Environment, La Jolla, CA 92037, USAFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil km 15.5, 97100 Mérida, Yucatán, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoFacultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México, Colonia Centro, 50000 Toluca, Estado de México, MexicoThis paper aims to describe the construction and operation of a respiration chamber of the head-box type for methane (CH<sub>4</sub>) measurements in bovines. The system consists of (1) a head box with a stainless steel frame and acrylic walls, floor, and ceiling; (2) a stainless steel feeder; (3) an automatic drinking water bowl; (4) a hood made from reinforced canvas; (5) an infrared (IR) CH<sub>4</sub> gas analyzer, a mass flow generator, a data-acquisition system; and (6) a steel metabolic box. Six assays were conducted to determine the pure CH<sub>4</sub> recovery rate of the whole system in order to validate it and comply with standards of chamber operation. The gravimetrical method was used for the recovery test and the recovery rate obtained was 1.04 ± 0.05. Once the system was calibrated, measurements of CH<sub>4</sub> were conducted using eight animals consisting of four Holstein cows with a live weight of 593.8 ± 51 kg and an average milk yield of 23.3 ± 1.8 kg d<sup>−1</sup> and four heifers with a live weight of 339 ± 28 kg. The CH<sub>4</sub> production values were 687 ± 123 and 248 ± 40 L CH<sub>4</sub> d<sup>−1</sup> for cows and heifers, respectively. The CH<sub>4</sub> yield was 19.7 ± 3.4 g and 17.1 ± 3.4 g CH<sub>4</sub> kg<sup>−1</sup> of dry matter consumed for cows and heifers, respectively. These results are consistent with those reported in the literature.https://www.mdpi.com/2076-2615/10/2/227respirometry systemym factoremission factorcowsheifers |
| spellingShingle | Octavio Alonso Castelán Ortega Paulina Elizabeth Pedraza Beltrán Gloria Stefanny Hernández Pineda Mohammed Benaouda Manuel González Ronquillo Luisa T Molina Juan Carlos Ku Vera Hugo Daniel Montelongo Pérez María Fernanda Vázquez Carrillo Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle Animals respirometry system ym factor emission factor cows heifers |
| title | Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle |
| title_full | Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle |
| title_fullStr | Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle |
| title_full_unstemmed | Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle |
| title_short | Construction and Operation of a Respiration Chamber of the Head-Box Type for Methane Measurement from Cattle |
| title_sort | construction and operation of a respiration chamber of the head box type for methane measurement from cattle |
| topic | respirometry system ym factor emission factor cows heifers |
| url | https://www.mdpi.com/2076-2615/10/2/227 |
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