Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo
Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1513994 |
| _version_ | 1818840096891207680 |
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| author | Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang |
| author_facet | Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang |
| author_sort | Lei Xiu |
| collection | DOAJ |
| description | Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4+T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDV-specific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines. |
| first_indexed | 2024-12-19T04:04:46Z |
| format | Article |
| id | doaj.art-7c3d42cc2c04429bac7b4c46a1104ec4 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-19T04:04:46Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-7c3d42cc2c04429bac7b4c46a1104ec42022-12-21T20:36:33ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-012911086110510.1080/09540105.2018.15139941513994Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivoLei Xiu0Haochi Zhang1Zhongpeng Hu2Yanchen Liang3Shuai Guo4Ming Yang5Ruiping Du6Xiao Wang7State Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityState Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityState Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityState Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityState Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityThe Biodesign Institute, Arizona State UniversityAnimal Nutrition Institute, Agriculture and Animal Husbandry Academy of Inner MongoliaState Key Laboratory of Reproductive Regulation & Breding of Grassland Livestock, School of life Science, Inner Mongolia UniversityExopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4+T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDV-specific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.http://dx.doi.org/10.1080/09540105.2018.1513994exopolysaccharideslactobacillus caseiadjuvantimmune responses |
| spellingShingle | Lei Xiu Haochi Zhang Zhongpeng Hu Yanchen Liang Shuai Guo Ming Yang Ruiping Du Xiao Wang Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo Food and Agricultural Immunology exopolysaccharides lactobacillus casei adjuvant immune responses |
| title | Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo |
| title_full | Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo |
| title_fullStr | Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo |
| title_full_unstemmed | Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo |
| title_short | Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo |
| title_sort | immunostimulatory activity of exopolysaccharides from probiotic lactobacillus casei wxd030 strain as a novel adjuvant in vitro and in vivo |
| topic | exopolysaccharides lactobacillus casei adjuvant immune responses |
| url | http://dx.doi.org/10.1080/09540105.2018.1513994 |
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