The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols
Background and Aim: To enhance the reproductive potential and increase productivity and population of cows, spermatozoa sex-sorting technology is required. This study aimed to examine the effect of sexing sperm, separated using a bovine serum albumin (BSA) column with varying incubation durations an...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-03-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/March-2023/25.pdf |
| _version_ | 1797859763106611200 |
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| author | Langgeng Priyanto Herdis Herdis Santoso Santoso Rahma Isartina Anwar Tri Puji Priyatno Pradita Iustitia Sitaresmi Faiz Azhari Muhammad Gunawan Oktora Dwi Putranti |
| author_facet | Langgeng Priyanto Herdis Herdis Santoso Santoso Rahma Isartina Anwar Tri Puji Priyatno Pradita Iustitia Sitaresmi Faiz Azhari Muhammad Gunawan Oktora Dwi Putranti |
| author_sort | Langgeng Priyanto |
| collection | DOAJ |
| description | Background and Aim: To enhance the reproductive potential and increase productivity and population of cows, spermatozoa sex-sorting technology is required. This study aimed to examine the effect of sexing sperm, separated using a bovine serum albumin (BSA) column with varying incubation durations and centrifugation methods, for successful artificial insemination.
Materials and Methods: Six Simmental bulls and 30 cows (n = 30) as the recipients were selected for this study at Balai Pembibitaan Hijauan Pakan Ternak Sembawa Indonesia. The study parameters included sperm motility, viability, plasma membrane integrity, and conception rate (CR). The experiment was divided into three protocols to find out differences in some parameters: (1) BSA incubation time effect (P) with P1 (40 min), P2 (50 min), and P3 (60 min); (2) freezing time effect with before freezing and after-thawing treatments; and (3) CR determined by measuring the proportion of pregnant cows following insemination with non-sexed, X-bearing, and Y-bearing sperms without centrifugation (n = 15) (A0, A1, and A2) and with centrifugation (n = 15) (B0, B1, and B2) in the acquired data, which were counted using the Statistical Package for the Social Sciences version 21 program. Analysis of variance was utilized to evaluate all treatments at various levels.
Results: The results demonstrated that centrifugation time influenced all sperm quality metrics for sperm containing X and Y (p < 0.05). The non-return rate (NRR) of non-sexed frozen semen, both centrifuged (A0) and not centrifuged (B0), was more significant than frozen semen produced by sexing X and Y spermatozoa. The NRR indicated a value of 80% based on the number of lactating cows.
Conclusion: Bovine serum albumin incubation and centrifugation protocols influenced and decreased all sperm quality indicators throughout the sexing procedure and could still be used as a sexing protocol. Furthermore, regarding NRR and service per conception, non-sexual treatment is superior to sexing treatment. |
| first_indexed | 2024-04-09T21:35:54Z |
| format | Article |
| id | doaj.art-7c40a956e1434b6dbb2bc1c23af7bc69 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-09T21:35:54Z |
| publishDate | 2023-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-7c40a956e1434b6dbb2bc1c23af7bc692023-03-26T15:25:00ZengVeterinary WorldVeterinary World0972-89882231-09162023-03-0116363163710.14202/vetworld.2023.631-637The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocolsLanggeng Priyanto0https://orcid.org/0000-0001-6776-664XHerdis Herdis1https://orcid.org/0000-0002-1586-2960Santoso Santoso2https://orcid.org/0000-0002-6873-8197Rahma Isartina Anwar3https://orcid.org/0000-0002-5664-955XTri Puji Priyatno4https://orcid.org/0000-0003-0473-6851Pradita Iustitia Sitaresmi5https://orcid.org/0000-0003-3640-5184Faiz Azhari6https://orcid.org/0000-0001-6002-9954Muhammad Gunawan7https://orcid.org/0000-0002-7940-0028Oktora Dwi Putranti8https://orcid.org/0000-0001-5532-9944Department of Animal Science, Faculty of Agriculture, Sriwijaya University, South Sumatra, 30862, Indonesia; Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Department of Animal Science, Faculty of Agriculture, Sriwijaya University, South Sumatra, 30862, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.Department of Animal Husbandry, Faculty of Agriculture, Animal Husbandry of Universitas Khairun, Ternate, North Maluku, Indonesia.Background and Aim: To enhance the reproductive potential and increase productivity and population of cows, spermatozoa sex-sorting technology is required. This study aimed to examine the effect of sexing sperm, separated using a bovine serum albumin (BSA) column with varying incubation durations and centrifugation methods, for successful artificial insemination. Materials and Methods: Six Simmental bulls and 30 cows (n = 30) as the recipients were selected for this study at Balai Pembibitaan Hijauan Pakan Ternak Sembawa Indonesia. The study parameters included sperm motility, viability, plasma membrane integrity, and conception rate (CR). The experiment was divided into three protocols to find out differences in some parameters: (1) BSA incubation time effect (P) with P1 (40 min), P2 (50 min), and P3 (60 min); (2) freezing time effect with before freezing and after-thawing treatments; and (3) CR determined by measuring the proportion of pregnant cows following insemination with non-sexed, X-bearing, and Y-bearing sperms without centrifugation (n = 15) (A0, A1, and A2) and with centrifugation (n = 15) (B0, B1, and B2) in the acquired data, which were counted using the Statistical Package for the Social Sciences version 21 program. Analysis of variance was utilized to evaluate all treatments at various levels. Results: The results demonstrated that centrifugation time influenced all sperm quality metrics for sperm containing X and Y (p < 0.05). The non-return rate (NRR) of non-sexed frozen semen, both centrifuged (A0) and not centrifuged (B0), was more significant than frozen semen produced by sexing X and Y spermatozoa. The NRR indicated a value of 80% based on the number of lactating cows. Conclusion: Bovine serum albumin incubation and centrifugation protocols influenced and decreased all sperm quality indicators throughout the sexing procedure and could still be used as a sexing protocol. Furthermore, regarding NRR and service per conception, non-sexual treatment is superior to sexing treatment.https://www.veterinaryworld.org/Vol.16/March-2023/25.pdfbovine serum albumincentrifugatedconception rateincubationsexingsperm |
| spellingShingle | Langgeng Priyanto Herdis Herdis Santoso Santoso Rahma Isartina Anwar Tri Puji Priyatno Pradita Iustitia Sitaresmi Faiz Azhari Muhammad Gunawan Oktora Dwi Putranti The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols Veterinary World bovine serum albumin centrifugated conception rate incubation sexing sperm |
| title | The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols |
| title_full | The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols |
| title_fullStr | The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols |
| title_full_unstemmed | The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols |
| title_short | The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols |
| title_sort | reproductive success of simmental bovine after sex sorting under various incubation and centrifugation protocols |
| topic | bovine serum albumin centrifugated conception rate incubation sexing sperm |
| url | https://www.veterinaryworld.org/Vol.16/March-2023/25.pdf |
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