Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Testing HIV-1 RNA in plasma by PCR is universally accepted as the ultimate standard to confirm diagnosis of HIV-1 infection and to monitor viral load in patients under treatment. However, in some cases, this assay could either underestimate or overestimate the replication capacity of a circulating o...

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Bibliographic Details
Main Authors: Beatrice Macchi, Caterina Frezza, Francesca Marino-Merlo, Antonella Minutolo, Valeria Stefanizzi, Emanuela Balestrieri, Carlotta Cerva, Loredana Sarmati, Massimo Andreoni, Sandro Grelli, Antonio Mastino
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Pathogens
Subjects:
human immunodeficiency virus
HIV diagnostics
viral load
reverse transcriptase
quantitative PCR assay
Online Access:https://www.mdpi.com/2076-0817/9/12/1047

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