Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making
Abstract Background Despite recent advances in research, there are still critical lacunae in our basic understanding of the cause, pathogenesis, and natural history of many cancers, especially heterogeneity in patient response to drugs and mediators in the transition from malignant to invasive pheno...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-07-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-11078-9 |
| _version_ | 1827633976126210048 |
|---|---|
| author | Swati Chitrangi Pooja Vaity Aishwarya Jamdar Shweta Bhatt |
| author_facet | Swati Chitrangi Pooja Vaity Aishwarya Jamdar Shweta Bhatt |
| author_sort | Swati Chitrangi |
| collection | DOAJ |
| description | Abstract Background Despite recent advances in research, there are still critical lacunae in our basic understanding of the cause, pathogenesis, and natural history of many cancers, especially heterogeneity in patient response to drugs and mediators in the transition from malignant to invasive phenotypes. The explication of the pathogenesis of cancer has been constrained by limited access to patient samples, tumor heterogeneity and lack of reliable biological models. Amelioration in cancer treatment depends on further understanding of the etiologic, genetic, biological, and clinical heterogeneity of tumor microenvironment. Patient-derived organoids recapitulate the basic features of primary tumors, including histological complexity and genetic heterogeneity, which is instrumental in predicting patient response to drugs. Methods Human iPSCs from healthy donors, breast and ovarian cancer patients were successfully differentiated towards isogenic hepatic, cardiac, neural and endothelial lineages. Multicellular organoids were established using Primary cells isolated from tumor tissues, histologically normal tissues adjacent to the tumors (NATs) and adipose tissues (source of Mesenchymal Stem Cells) from ovarian and breast cancer patients. Further these organoids were propagated and used for drug resistance/sensitivity studies. Results Ovarian and breast cancer patients’ organoids showed heterogeneity in drug resistance and sensitivity. iPSCs-derived cardiomyocytes, hepatocytes and neurons showed donor–to-donor variability of chemotherapeutic drug sensitivity in ovarian cancer patients, breast cancer patients and healthy donors. Conclusion We report development of a novel integrated platform to facilitate clinical decision-making using the patient's primary cells, iPSCs and derivatives, to clinically relevant models for oncology research. |
| first_indexed | 2024-03-09T15:06:48Z |
| format | Article |
| id | doaj.art-7c439c97054f4a7686f41d0c19c3919d |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-09T15:06:48Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-7c439c97054f4a7686f41d0c19c3919d2023-11-26T13:36:03ZengBMCBMC Cancer1471-24072023-07-0123112410.1186/s12885-023-11078-9Patient-derived organoids for precision oncology: a platform to facilitate clinical decision makingSwati Chitrangi0Pooja Vaity1Aishwarya Jamdar2Shweta Bhatt3Department of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDCDepartment of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDCDepartment of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDCDepartment of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDCAbstract Background Despite recent advances in research, there are still critical lacunae in our basic understanding of the cause, pathogenesis, and natural history of many cancers, especially heterogeneity in patient response to drugs and mediators in the transition from malignant to invasive phenotypes. The explication of the pathogenesis of cancer has been constrained by limited access to patient samples, tumor heterogeneity and lack of reliable biological models. Amelioration in cancer treatment depends on further understanding of the etiologic, genetic, biological, and clinical heterogeneity of tumor microenvironment. Patient-derived organoids recapitulate the basic features of primary tumors, including histological complexity and genetic heterogeneity, which is instrumental in predicting patient response to drugs. Methods Human iPSCs from healthy donors, breast and ovarian cancer patients were successfully differentiated towards isogenic hepatic, cardiac, neural and endothelial lineages. Multicellular organoids were established using Primary cells isolated from tumor tissues, histologically normal tissues adjacent to the tumors (NATs) and adipose tissues (source of Mesenchymal Stem Cells) from ovarian and breast cancer patients. Further these organoids were propagated and used for drug resistance/sensitivity studies. Results Ovarian and breast cancer patients’ organoids showed heterogeneity in drug resistance and sensitivity. iPSCs-derived cardiomyocytes, hepatocytes and neurons showed donor–to-donor variability of chemotherapeutic drug sensitivity in ovarian cancer patients, breast cancer patients and healthy donors. Conclusion We report development of a novel integrated platform to facilitate clinical decision-making using the patient's primary cells, iPSCs and derivatives, to clinically relevant models for oncology research.https://doi.org/10.1186/s12885-023-11078-9Patient-Derived Organoids (PDOs)Tumor Microenvironment (TME)Patient-derived Xenograft (PDX)Drug efficacy and safety studiesPatient-derived induced pluripotent stem cells (iPSCs) |
| spellingShingle | Swati Chitrangi Pooja Vaity Aishwarya Jamdar Shweta Bhatt Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making BMC Cancer Patient-Derived Organoids (PDOs) Tumor Microenvironment (TME) Patient-derived Xenograft (PDX) Drug efficacy and safety studies Patient-derived induced pluripotent stem cells (iPSCs) |
| title | Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making |
| title_full | Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making |
| title_fullStr | Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making |
| title_full_unstemmed | Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making |
| title_short | Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making |
| title_sort | patient derived organoids for precision oncology a platform to facilitate clinical decision making |
| topic | Patient-Derived Organoids (PDOs) Tumor Microenvironment (TME) Patient-derived Xenograft (PDX) Drug efficacy and safety studies Patient-derived induced pluripotent stem cells (iPSCs) |
| url | https://doi.org/10.1186/s12885-023-11078-9 |
| work_keys_str_mv | AT swatichitrangi patientderivedorganoidsforprecisiononcologyaplatformtofacilitateclinicaldecisionmaking AT poojavaity patientderivedorganoidsforprecisiononcologyaplatformtofacilitateclinicaldecisionmaking AT aishwaryajamdar patientderivedorganoidsforprecisiononcologyaplatformtofacilitateclinicaldecisionmaking AT shwetabhatt patientderivedorganoidsforprecisiononcologyaplatformtofacilitateclinicaldecisionmaking |