Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response
Thylakoid membranes derived from green leaf chloroplasts affect appetite-regulating hormones, suppress food intake, reduce blood lipids and lead to a decreased body weight in animals and human subjects. Thylakoids also decrease the intestinal in vitro uptake of methyl-glucose in the rat. The aim of...
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Cambridge University Press
2013-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679012000298/type/journal_article |
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| author | Caroline Montelius Nadia Osman Björn Weström Siv Ahrné Göran Molin Per-Åke Albertsson Charlotte Erlanson-Albertsson |
| author_facet | Caroline Montelius Nadia Osman Björn Weström Siv Ahrné Göran Molin Per-Åke Albertsson Charlotte Erlanson-Albertsson |
| author_sort | Caroline Montelius |
| collection | DOAJ |
| description | Thylakoid membranes derived from green leaf chloroplasts affect appetite-regulating hormones, suppress food intake, reduce blood lipids and lead to a decreased body weight in animals and human subjects. Thylakoids also decrease the intestinal in vitro uptake of methyl-glucose in the rat. The aim of this study was to investigate the effect of dietary thylakoids on the gut microbiota composition, mainly the taxa of lactobacilli and bifidobacteria, in rats fed either a thylakoid-enriched diet or a control diet for 10 d. At the same time, a glucose-tolerance test in the same rats was also performed. Food intake was significantly decreased in the thylakoid-fed rats compared with the control-fed rats over the 10-d study. An oral glucose tolerance test after 10 d of thylakoid- or control-food intake resulted in significantly reduced plasma insulin levels in the thylakoid-fed rats compared with the control-fed rats, while no difference was observed for blood glucose levels. Analysis of gut bacteria showed a significant increase of lactobacilli on the ileal mucosa, specifically Lactobacillus reuteri, in the rats fed the thylakoid diet compared with rats fed the control diet, while faecal lactobacilli decreased. No difference in bifidobacteria between the thylakoid and control groups was found. Analyses with terminal restriction fragment length polymorphism and principal component analysis of faeces demonstrated different microbial populations in the thylakoid- and control-fed animals. These findings indicate that thylakoids modulate the gut microbial composition, which might be important for the regulation of body weight and energy metabolism. |
| first_indexed | 2024-04-10T04:44:03Z |
| format | Article |
| id | doaj.art-7c43fd62a1a94ab8ab243fa5026f8d01 |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:44:03Z |
| publishDate | 2013-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-7c43fd62a1a94ab8ab243fa5026f8d012023-03-09T12:38:38ZengCambridge University PressJournal of Nutritional Science2048-67902013-01-01210.1017/jns.2012.29Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin responseCaroline Montelius0Nadia Osman1Björn Weström2Siv Ahrné3Göran Molin4Per-Åke Albertsson5Charlotte Erlanson-Albertsson6Department of Experimental Medical Science, Lund University, Lund, SwedenDepartment of Experimental Medical Science, Lund University, Lund, SwedenDepartment of Biology, Lund University, Lund, SwedenDepartment of Food Technology, Engineering and Nutrition, Lund University, Lund, SwedenDepartment of Food Technology, Engineering and Nutrition, Lund University, Lund, SwedenDepartment of Biochemistry and Structural Biology, Lund University, Lund, SwedenDepartment of Experimental Medical Science, Lund University, Lund, SwedenThylakoid membranes derived from green leaf chloroplasts affect appetite-regulating hormones, suppress food intake, reduce blood lipids and lead to a decreased body weight in animals and human subjects. Thylakoids also decrease the intestinal in vitro uptake of methyl-glucose in the rat. The aim of this study was to investigate the effect of dietary thylakoids on the gut microbiota composition, mainly the taxa of lactobacilli and bifidobacteria, in rats fed either a thylakoid-enriched diet or a control diet for 10 d. At the same time, a glucose-tolerance test in the same rats was also performed. Food intake was significantly decreased in the thylakoid-fed rats compared with the control-fed rats over the 10-d study. An oral glucose tolerance test after 10 d of thylakoid- or control-food intake resulted in significantly reduced plasma insulin levels in the thylakoid-fed rats compared with the control-fed rats, while no difference was observed for blood glucose levels. Analysis of gut bacteria showed a significant increase of lactobacilli on the ileal mucosa, specifically Lactobacillus reuteri, in the rats fed the thylakoid diet compared with rats fed the control diet, while faecal lactobacilli decreased. No difference in bifidobacteria between the thylakoid and control groups was found. Analyses with terminal restriction fragment length polymorphism and principal component analysis of faeces demonstrated different microbial populations in the thylakoid- and control-fed animals. These findings indicate that thylakoids modulate the gut microbial composition, which might be important for the regulation of body weight and energy metabolism.https://www.cambridge.org/core/product/identifier/S2048679012000298/type/journal_articleColonSmall intestineObesityLactobacilliQuantitative PCR |
| spellingShingle | Caroline Montelius Nadia Osman Björn Weström Siv Ahrné Göran Molin Per-Åke Albertsson Charlotte Erlanson-Albertsson Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response Journal of Nutritional Science Colon Small intestine Obesity Lactobacilli Quantitative PCR |
| title | Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response |
| title_full | Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response |
| title_fullStr | Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response |
| title_full_unstemmed | Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response |
| title_short | Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response |
| title_sort | feeding spinach thylakoids to rats modulates the gut microbiota decreases food intake and affects the insulin response |
| topic | Colon Small intestine Obesity Lactobacilli Quantitative PCR |
| url | https://www.cambridge.org/core/product/identifier/S2048679012000298/type/journal_article |
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