The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer
Purpose of the study. Determine the content of cancer stem cells (CSCs) in the tumor tissue (TT) and perifocal tissues (PT) in muscle-non-invasive bladder cancer.Materials and methods. We’ve examined fragments of TT and PT of 7 muscle-non-invasive bladder cancer (NMIBC) after surgical intervention –...
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| Format: | Article |
| Language: | Russian |
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ANO "Perspective of oncology"
2022-03-01
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| Series: | Южно-Российский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.cancersp.com/jour/article/view/137 |
| _version_ | 1797882885355601920 |
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| author | L. I. Belyakova A. N. Shevchenko A. B. Sagakyants E. S. Bondarenko O. G. Shulgina E. P. Ulyanova, E. V. Filatova I. A. Khomutenko |
| author_facet | L. I. Belyakova A. N. Shevchenko A. B. Sagakyants E. S. Bondarenko O. G. Shulgina E. P. Ulyanova, E. V. Filatova I. A. Khomutenko |
| author_sort | L. I. Belyakova |
| collection | DOAJ |
| description | Purpose of the study. Determine the content of cancer stem cells (CSCs) in the tumor tissue (TT) and perifocal tissues (PT) in muscle-non-invasive bladder cancer.Materials and methods. We’ve examined fragments of TT and PT of 7 muscle-non-invasive bladder cancer (NMIBC) after surgical intervention – transurethral resection of the urinary bladder (TUR). In tissue samples that were used to obtain cell suspension of TT and PT using the BD Medimachine apparatus (BD, USA) was treated with monoclonal antibodies CD45-APCCy7, CD44-FITC, CD133-РЕ, CD24-PE (BD, USA) and were assessed on flow cytometer FacsCantoII (BD, USA). The percentage of cells with CSC phenotypic markers was determined in the analysis sample: CD45-CD44+CD24+, CD45-CD44+, CD45-CD24+, CD45-CD133+, CD45-CD44+CD133+. The presence of significant differences in the groups was evaluated using the STATISTICA 13 software package and the differences between the samples were considered significant at p < 0.05. The percentage of cells of the corresponding phenotype was calculated relative to the total number of cells. The percentage of cells with the corresponding phenotype was calculated relative to the total number of cells.Results. The relative numbers of cells with CSC phenotypic markers, such as CD24, CD44, were 77 % and 58 % higher in TT than in PT: 18.3 ± 3.5 vs. 4.3 ± 2.1, p ≤ 0.044 and 15.5 ± 5.3 vs. 6.5 ± 0.8, p ≤ 0.043, respectively. The number of CD133+ cells was 83 % higher in PT compared to TT – 41.6 ± 12.1 vs. 22.7 ± 7.6, p ≤ 0.047.Conclusion. The study of CSCs is a promising direction for the study of oncogenesis and can be used to assess the nature of the further development of relapse and / or progression of the disease, as well as various therapeutic approaches that are aimed at eliminating with CSC phenotypic markers and blocking the pathways leading to the emergence and maintenance of this cell population in patients with NMIBC. |
| first_indexed | 2024-04-10T03:41:42Z |
| format | Article |
| id | doaj.art-7c44049153ea4147877148ce8a15ce7d |
| institution | Directory Open Access Journal |
| issn | 2686-9039 |
| language | Russian |
| last_indexed | 2024-04-10T03:41:42Z |
| publishDate | 2022-03-01 |
| publisher | ANO "Perspective of oncology" |
| record_format | Article |
| series | Южно-Российский онкологический журнал |
| spelling | doaj.art-7c44049153ea4147877148ce8a15ce7d2023-03-13T07:19:28ZrusANO "Perspective of oncology"Южно-Российский онкологический журнал2686-90392022-03-013161410.37748/2686-9039-2022-3-1-187The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancerL. I. Belyakova0A. N. Shevchenko1A. B. Sagakyants2E. S. Bondarenko3O. G. Shulgina4E. P. Ulyanova,5E. V. Filatova6I. A. Khomutenko7НМИЦ онкологииНМИЦ онкологииНМИЦ онкологииНМИЦ онкологииНМИЦ онкологииНМИЦ онкологииНМИЦ онкологииНМИЦ онкологииPurpose of the study. Determine the content of cancer stem cells (CSCs) in the tumor tissue (TT) and perifocal tissues (PT) in muscle-non-invasive bladder cancer.Materials and methods. We’ve examined fragments of TT and PT of 7 muscle-non-invasive bladder cancer (NMIBC) after surgical intervention – transurethral resection of the urinary bladder (TUR). In tissue samples that were used to obtain cell suspension of TT and PT using the BD Medimachine apparatus (BD, USA) was treated with monoclonal antibodies CD45-APCCy7, CD44-FITC, CD133-РЕ, CD24-PE (BD, USA) and were assessed on flow cytometer FacsCantoII (BD, USA). The percentage of cells with CSC phenotypic markers was determined in the analysis sample: CD45-CD44+CD24+, CD45-CD44+, CD45-CD24+, CD45-CD133+, CD45-CD44+CD133+. The presence of significant differences in the groups was evaluated using the STATISTICA 13 software package and the differences between the samples were considered significant at p < 0.05. The percentage of cells of the corresponding phenotype was calculated relative to the total number of cells. The percentage of cells with the corresponding phenotype was calculated relative to the total number of cells.Results. The relative numbers of cells with CSC phenotypic markers, such as CD24, CD44, were 77 % and 58 % higher in TT than in PT: 18.3 ± 3.5 vs. 4.3 ± 2.1, p ≤ 0.044 and 15.5 ± 5.3 vs. 6.5 ± 0.8, p ≤ 0.043, respectively. The number of CD133+ cells was 83 % higher in PT compared to TT – 41.6 ± 12.1 vs. 22.7 ± 7.6, p ≤ 0.047.Conclusion. The study of CSCs is a promising direction for the study of oncogenesis and can be used to assess the nature of the further development of relapse and / or progression of the disease, as well as various therapeutic approaches that are aimed at eliminating with CSC phenotypic markers and blocking the pathways leading to the emergence and maintenance of this cell population in patients with NMIBC.https://www.cancersp.com/jour/article/view/137мышечно-неинвазивный рак мочевого пузыряопухолевые стволовые клеткиперитуморальная зона |
| spellingShingle | L. I. Belyakova A. N. Shevchenko A. B. Sagakyants E. S. Bondarenko O. G. Shulgina E. P. Ulyanova, E. V. Filatova I. A. Khomutenko The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer Южно-Российский онкологический журнал мышечно-неинвазивный рак мочевого пузыря опухолевые стволовые клетки перитуморальная зона |
| title | The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer |
| title_full | The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer |
| title_fullStr | The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer |
| title_full_unstemmed | The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer |
| title_short | The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer |
| title_sort | number of cancer stem cells in the tumor tissue and perifocal tissue of non muscle invasive bladder cancer |
| topic | мышечно-неинвазивный рак мочевого пузыря опухолевые стволовые клетки перитуморальная зона |
| url | https://www.cancersp.com/jour/article/view/137 |
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