Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro
Sebaceous gland cells (sebocytes) differentiate to intracellularly accumulate lipid droplets – a phenomenon similar to that found in adipocytes. In the present study, we examined whether the regulation of lipogenesis in sebocytes is the same as that in preadipocytes. When sebocytes and preadipocytes...
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| Language: | English |
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Elsevier
2020-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580820300704 |
| _version_ | 1819076933470650368 |
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| author | Takashi Sato Fusatoshi Shibata Toshikazu Koiwai Noriko Akimoto |
| author_facet | Takashi Sato Fusatoshi Shibata Toshikazu Koiwai Noriko Akimoto |
| author_sort | Takashi Sato |
| collection | DOAJ |
| description | Sebaceous gland cells (sebocytes) differentiate to intracellularly accumulate lipid droplets – a phenomenon similar to that found in adipocytes. In the present study, we examined whether the regulation of lipogenesis in sebocytes is the same as that in preadipocytes. When sebocytes and preadipocytes, prepared from auricle and subcutaneous adipose tissues from the inguinal region of hamsters, respectively, were treated with a common differentiation inducer, insulin, intracellular lipid-droplet formation and triacyglycerol (TG) production were dose- and time-dependently augmented in both. Insulin increased the production of perilipin, a differentiation marker in both sebocytes and adipocytes. Insulin-like growth factor 1 (IGF-1) augmented the intracellular level of TG in sebocytes and preadipocytes. In addition, the action of 1α,25-dihydroxyvitamin D3 [1,25(OH2)D3] on TG production was the opposite between sebocytes and preadipocytes. Furthermore, 5α-dihydrotestosterone (5α-DHT) augmented the TG level in sebocytes, whereas it did not alter TG production in preadipocytes. Moreover, insulin-augmented TG production in sebocytes was enhanced by IGF-1 and 5α-DHT, while diminished by 1,25(OH2)D3. In preadipocytes, the insulin-augmented production of TG was decreased by IGF-1, 1,25(OH2)D3, and 5α-DHT. These results suggest that sebocytic lipogenesis is partially similar to but substantially different from adipocyte lipogenesis due to the forementioned hormones and growth factors in the skin under physiological conditions. |
| first_indexed | 2024-12-21T18:49:11Z |
| format | Article |
| id | doaj.art-7c45fa52418e455fb99dab35bbae0c6c |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-21T18:49:11Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-7c45fa52418e455fb99dab35bbae0c6c2022-12-21T18:53:48ZengElsevierBiochemistry and Biophysics Reports2405-58082020-07-0122Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitroTakashi Sato0Fusatoshi Shibata1Toshikazu Koiwai2Noriko Akimoto3Corresponding author. Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.; Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, 192-0392, JapanDepartment of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, 192-0392, JapanDepartment of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, 192-0392, JapanDepartment of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, 192-0392, JapanSebaceous gland cells (sebocytes) differentiate to intracellularly accumulate lipid droplets – a phenomenon similar to that found in adipocytes. In the present study, we examined whether the regulation of lipogenesis in sebocytes is the same as that in preadipocytes. When sebocytes and preadipocytes, prepared from auricle and subcutaneous adipose tissues from the inguinal region of hamsters, respectively, were treated with a common differentiation inducer, insulin, intracellular lipid-droplet formation and triacyglycerol (TG) production were dose- and time-dependently augmented in both. Insulin increased the production of perilipin, a differentiation marker in both sebocytes and adipocytes. Insulin-like growth factor 1 (IGF-1) augmented the intracellular level of TG in sebocytes and preadipocytes. In addition, the action of 1α,25-dihydroxyvitamin D3 [1,25(OH2)D3] on TG production was the opposite between sebocytes and preadipocytes. Furthermore, 5α-dihydrotestosterone (5α-DHT) augmented the TG level in sebocytes, whereas it did not alter TG production in preadipocytes. Moreover, insulin-augmented TG production in sebocytes was enhanced by IGF-1 and 5α-DHT, while diminished by 1,25(OH2)D3. In preadipocytes, the insulin-augmented production of TG was decreased by IGF-1, 1,25(OH2)D3, and 5α-DHT. These results suggest that sebocytic lipogenesis is partially similar to but substantially different from adipocyte lipogenesis due to the forementioned hormones and growth factors in the skin under physiological conditions.http://www.sciencedirect.com/science/article/pii/S2405580820300704SebocytesLipogenesisPreadipocytesTriacyglycerolLipid-droplet formation |
| spellingShingle | Takashi Sato Fusatoshi Shibata Toshikazu Koiwai Noriko Akimoto Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro Biochemistry and Biophysics Reports Sebocytes Lipogenesis Preadipocytes Triacyglycerol Lipid-droplet formation |
| title | Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| title_full | Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| title_fullStr | Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| title_full_unstemmed | Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| title_short | Different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| title_sort | different regulation of lipogenesis in sebocytes and subcutaneous preadipocytes in hamsters in vitro |
| topic | Sebocytes Lipogenesis Preadipocytes Triacyglycerol Lipid-droplet formation |
| url | http://www.sciencedirect.com/science/article/pii/S2405580820300704 |
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