Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines
Potential drug-eluting scaffolds of electrospun poly(acrylic acid-<i>co</i>-styrene sulfonate) P(AA-<i>co</i>-SS) in clonogenic assays using tumorigenic gastric and ovarian cancer cells were tested in vitro. Electrospun polymer nanofiber (EPnF) meshes of PAA and PSSNa homo- a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/12/21/3903 |
| _version_ | 1827645860181180416 |
|---|---|
| author | Andrónico Neira-Carrillo Ignacio A. Zárate Eddie Nieto Nicole Butto-Miranda Lorena Lobos-González Matias Del Campo-Smith Daniel A. Palacio Bruno F. Urbano |
| author_facet | Andrónico Neira-Carrillo Ignacio A. Zárate Eddie Nieto Nicole Butto-Miranda Lorena Lobos-González Matias Del Campo-Smith Daniel A. Palacio Bruno F. Urbano |
| author_sort | Andrónico Neira-Carrillo |
| collection | DOAJ |
| description | Potential drug-eluting scaffolds of electrospun poly(acrylic acid-<i>co</i>-styrene sulfonate) P(AA-<i>co</i>-SS) in clonogenic assays using tumorigenic gastric and ovarian cancer cells were tested in vitro. Electrospun polymer nanofiber (EPnF) meshes of PAA and PSSNa homo- and P(AA-<i>co</i>-SS) copolymer composed of 30:70, 50:50, 70:30 acrylic acid (AA) and sodium 4-styrene sulfonate (SSNa) units were performed by electrospinning (ES). The synthesis, structural and morphological characterization of all EPnF meshes were analyzed by optical and electron microscopy (SEM-EDS), infrared spectroscopy (FTIR), contact angle, and X-ray diffraction (XRD) measurements. This study shows that different ratio of AA and SSNa of monomers in P(AA-<i>co</i>-SS) EPnF play a crucial role in clonogenic in vitro assays. We found that 50:50 P(AA-<i>co</i>-SS) EPnF mesh loaded with antineoplastic drugs can be an excellent suppressor of growth-independent anchored capacities in vitro assays and a good subcutaneous drug delivery system for chemotherapeutic medication in vivo model for surgical resection procedures in cancer research. |
| first_indexed | 2024-03-09T18:47:03Z |
| format | Article |
| id | doaj.art-7c49eeb4ffd4496495d77f211b1acd0f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T18:47:03Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7c49eeb4ffd4496495d77f211b1acd0f2023-11-24T06:11:06ZengMDPI AGNanomaterials2079-49912022-11-011221390310.3390/nano12213903Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell LinesAndrónico Neira-Carrillo0Ignacio A. Zárate1Eddie Nieto2Nicole Butto-Miranda3Lorena Lobos-González4Matias Del Campo-Smith5Daniel A. Palacio6Bruno F. Urbano7Department of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, ChileAdvanced Center for Chronic Diseases (ACCDIS), Santiago 380492, ChileAdvanced Center for Chronic Diseases (ACCDIS), Santiago 380492, ChileDepartment of Polymer Chemistry, Faculty of Chemical Science, University of Concepción, Concepción 3349001, ChileDepartment of Polymer Chemistry, Faculty of Chemical Science, University of Concepción, Concepción 3349001, ChilePotential drug-eluting scaffolds of electrospun poly(acrylic acid-<i>co</i>-styrene sulfonate) P(AA-<i>co</i>-SS) in clonogenic assays using tumorigenic gastric and ovarian cancer cells were tested in vitro. Electrospun polymer nanofiber (EPnF) meshes of PAA and PSSNa homo- and P(AA-<i>co</i>-SS) copolymer composed of 30:70, 50:50, 70:30 acrylic acid (AA) and sodium 4-styrene sulfonate (SSNa) units were performed by electrospinning (ES). The synthesis, structural and morphological characterization of all EPnF meshes were analyzed by optical and electron microscopy (SEM-EDS), infrared spectroscopy (FTIR), contact angle, and X-ray diffraction (XRD) measurements. This study shows that different ratio of AA and SSNa of monomers in P(AA-<i>co</i>-SS) EPnF play a crucial role in clonogenic in vitro assays. We found that 50:50 P(AA-<i>co</i>-SS) EPnF mesh loaded with antineoplastic drugs can be an excellent suppressor of growth-independent anchored capacities in vitro assays and a good subcutaneous drug delivery system for chemotherapeutic medication in vivo model for surgical resection procedures in cancer research.https://www.mdpi.com/2079-4991/12/21/3903electrospun fiberselectrospinningpoly(acrylic acid-<i>co</i>-styrene sulfonate)gastric and breast cancer cellsclonogenic assaypolymers |
| spellingShingle | Andrónico Neira-Carrillo Ignacio A. Zárate Eddie Nieto Nicole Butto-Miranda Lorena Lobos-González Matias Del Campo-Smith Daniel A. Palacio Bruno F. Urbano Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines Nanomaterials electrospun fibers electrospinning poly(acrylic acid-<i>co</i>-styrene sulfonate) gastric and breast cancer cells clonogenic assay polymers |
| title | Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines |
| title_full | Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines |
| title_fullStr | Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines |
| title_full_unstemmed | Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines |
| title_short | Electrospun Poly(acrylic acid-<i>co</i>-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines |
| title_sort | electrospun poly acrylic acid i co i 4 styrene sulfonate as potential drug eluting scaffolds for targeted chemotherapeutic delivery systems on gastric ags and breast mda mb 231 cancer cell lines |
| topic | electrospun fibers electrospinning poly(acrylic acid-<i>co</i>-styrene sulfonate) gastric and breast cancer cells clonogenic assay polymers |
| url | https://www.mdpi.com/2079-4991/12/21/3903 |
| work_keys_str_mv | AT androniconeiracarrillo electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT ignacioazarate electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT eddienieto electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT nicolebuttomiranda electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT lorenalobosgonzalez electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT matiasdelcamposmith electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT danielapalacio electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines AT brunofurbano electrospunpolyacrylicacidicoi4styrenesulfonateaspotentialdrugelutingscaffoldsfortargetedchemotherapeuticdeliverysystemsongastricagsandbreastmdamb231cancercelllines |