Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro
Essentially employed for the treatment of airway obstructions in humans, β-agonists are also known to have an anabolic effect in animals’ skeletal muscle. In vivo and in vitro studies have attested the increase in animal body mass and the hypertrophy of muscle cells following the administration of s...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240558082100114X |
| _version_ | 1818622213047189504 |
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| author | Boimpoundi Eunice Flavie Ouali Hao-Ven Wang |
| author_facet | Boimpoundi Eunice Flavie Ouali Hao-Ven Wang |
| author_sort | Boimpoundi Eunice Flavie Ouali |
| collection | DOAJ |
| description | Essentially employed for the treatment of airway obstructions in humans, β-agonists are also known to have an anabolic effect in animals’ skeletal muscle. In vivo and in vitro studies have attested the increase in animal body mass and the hypertrophy of muscle cells following the administration of specific β-agonists. However, the contribution of β-agonists to C2C12 myoblasts growth remains obscure. We therefore aimed to investigate the impact of β1-and β2-agonist drugs on the proliferation and differentiation of skeletal muscle cells. Direct observations and cytotoxicity assay showed that clenbuterol, salbutamol, cimaterol and ractopamine enhanced muscle cell growth and viability during the proliferation stage. Structural examinations coupled to Western blot analysis indicated that salbutamol and cimaterol triggered a decrease in myotube formation. A better comprehension of the effect of β-agonists on myogenic regulatory genes in the muscle cells is crucial to establish a specific role of β-agonists in muscle development, growth, and regeneration. |
| first_indexed | 2024-12-16T18:21:35Z |
| format | Article |
| id | doaj.art-7c4f2b2e14424ff5aa3458c21da158dd |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-16T18:21:35Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-7c4f2b2e14424ff5aa3458c21da158dd2022-12-21T22:21:33ZengElsevierBiochemistry and Biophysics Reports2405-58082021-07-0126101019Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitroBoimpoundi Eunice Flavie Ouali0Hao-Ven Wang1Department of Life Sciences, College of Biosciences and Biotechnology, National Cheng Kung University, Tainan City, 701, Taiwan, ROC; Corresponding author. National Cheng Kung University, Department of Life Sciences, No.1 University Road, East Dist., Tainan City, 701, Taiwan, ROC.Department of Life Sciences, College of Biosciences and Biotechnology, National Cheng Kung University, Tainan City, 701, Taiwan, ROC; Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan City, 701, Taiwan, ROC; Marine Biology and Cetacean Research Center, National Cheng Kung University, Tainan City, 701, Taiwan, ROC; Corresponding author. National Cheng Kung University, Department of Life Sciences, No.1 University Road, East Dist., Tainan City, 701, Taiwan, ROC.Essentially employed for the treatment of airway obstructions in humans, β-agonists are also known to have an anabolic effect in animals’ skeletal muscle. In vivo and in vitro studies have attested the increase in animal body mass and the hypertrophy of muscle cells following the administration of specific β-agonists. However, the contribution of β-agonists to C2C12 myoblasts growth remains obscure. We therefore aimed to investigate the impact of β1-and β2-agonist drugs on the proliferation and differentiation of skeletal muscle cells. Direct observations and cytotoxicity assay showed that clenbuterol, salbutamol, cimaterol and ractopamine enhanced muscle cell growth and viability during the proliferation stage. Structural examinations coupled to Western blot analysis indicated that salbutamol and cimaterol triggered a decrease in myotube formation. A better comprehension of the effect of β-agonists on myogenic regulatory genes in the muscle cells is crucial to establish a specific role of β-agonists in muscle development, growth, and regeneration.http://www.sciencedirect.com/science/article/pii/S240558082100114XAgonist drugsC2C12ProliferationDifferentiation |
| spellingShingle | Boimpoundi Eunice Flavie Ouali Hao-Ven Wang Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro Biochemistry and Biophysics Reports Agonist drugs C2C12 Proliferation Differentiation |
| title | Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| title_full | Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| title_fullStr | Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| title_full_unstemmed | Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| title_short | Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| title_sort | beta agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro |
| topic | Agonist drugs C2C12 Proliferation Differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S240558082100114X |
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