Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture
In this study the effect of lipoprotein lipase (LPL) on the selective uptake of high density lipoprotein (HDL) cholesteryl esters (CE) by hepatic cells was investigated. Human HDL3 (d 1.125–1.21 g/ml) was radiolabeled with 125I in the protein moiety and with 3H in the CE moiety. LPL was prepared fro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1998-07-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520325141 |
| _version_ | 1818657218756608000 |
|---|---|
| author | Franz Rinninger Tatjana Kaiser W. Alexander Mann Nicolette Meyer Heiner Greten Ulrike Beisiegel |
| author_facet | Franz Rinninger Tatjana Kaiser W. Alexander Mann Nicolette Meyer Heiner Greten Ulrike Beisiegel |
| author_sort | Franz Rinninger |
| collection | DOAJ |
| description | In this study the effect of lipoprotein lipase (LPL) on the selective uptake of high density lipoprotein (HDL) cholesteryl esters (CE) by hepatic cells was investigated. Human HDL3 (d 1.125–1.21 g/ml) was radiolabeled with 125I in the protein moiety and with 3H in the CE moiety. LPL was prepared from bovine milk. Human hepatocytes in primary culture and human Hep3B hepatoma cells were incubated in medium containing doubly radiolabeled HDL3 with or without LPL. Without LPL, apparent HDL3 particle uptake according to the lipid tracer (3H) was in excess of that due to the protein label (125I) indicating selective CE uptake from HDL3. Addition of LPL increased selective CE uptake up to 7-fold. This stimulation of HDL3 selective CE uptake was independent of the lipolytic activity of LPL as suggested by several experimental approaches. Cell surface heparan sulfate proteoglycan deficiency decreased the LPL-mediated increase in selective CE uptake suggesting an important role for these molecules. In low density lipoprotein (LDL) receptor- or LDL receptor-related protein-(LRP)-deficient cells, LPL increased selective CE uptake as it did in normal cells yielding no evidence that these receptors play a role in the LPL effect on selective CE uptake. In summary, lipoprotein lipase increases the selective uptake of high density lipoprotein-associated cholesteryl ester by hepatic cells in culture. This effect is dependent on cell surface heparan sulfate proteoglycans but independent of lipolysis and of endocytosis mediated by low density lipoprotein receptor-related or low density lipoprotein receptors.—Rinninger, F., T. Kaiser, W. A. Mann, N. Meyer, H. Greten, and U. Beisiegel. Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture. J. Lipid Res. 1998. 39: 1335–1348. |
| first_indexed | 2024-12-17T03:37:59Z |
| format | Article |
| id | doaj.art-7c4fd503ab754a9f97a06914f72128a4 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T03:37:59Z |
| publishDate | 1998-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7c4fd503ab754a9f97a06914f72128a42022-12-21T22:05:05ZengElsevierJournal of Lipid Research0022-22751998-07-0139713351348Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in cultureFranz Rinninger0Tatjana Kaiser1W. Alexander Mann2Nicolette Meyer3Heiner Greten4Ulrike Beisiegel5To whom correspondence should be addressed.; Universität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyUniversität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyUniversität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyUniversität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyUniversität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyUniversität Hamburg, Krankenhaus Eppendorf, Medizinische Kernklinik und Poliklinik, Martinistrasse 52, 20246 Hamburg, GermanyIn this study the effect of lipoprotein lipase (LPL) on the selective uptake of high density lipoprotein (HDL) cholesteryl esters (CE) by hepatic cells was investigated. Human HDL3 (d 1.125–1.21 g/ml) was radiolabeled with 125I in the protein moiety and with 3H in the CE moiety. LPL was prepared from bovine milk. Human hepatocytes in primary culture and human Hep3B hepatoma cells were incubated in medium containing doubly radiolabeled HDL3 with or without LPL. Without LPL, apparent HDL3 particle uptake according to the lipid tracer (3H) was in excess of that due to the protein label (125I) indicating selective CE uptake from HDL3. Addition of LPL increased selective CE uptake up to 7-fold. This stimulation of HDL3 selective CE uptake was independent of the lipolytic activity of LPL as suggested by several experimental approaches. Cell surface heparan sulfate proteoglycan deficiency decreased the LPL-mediated increase in selective CE uptake suggesting an important role for these molecules. In low density lipoprotein (LDL) receptor- or LDL receptor-related protein-(LRP)-deficient cells, LPL increased selective CE uptake as it did in normal cells yielding no evidence that these receptors play a role in the LPL effect on selective CE uptake. In summary, lipoprotein lipase increases the selective uptake of high density lipoprotein-associated cholesteryl ester by hepatic cells in culture. This effect is dependent on cell surface heparan sulfate proteoglycans but independent of lipolysis and of endocytosis mediated by low density lipoprotein receptor-related or low density lipoprotein receptors.—Rinninger, F., T. Kaiser, W. A. Mann, N. Meyer, H. Greten, and U. Beisiegel. Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture. J. Lipid Res. 1998. 39: 1335–1348.http://www.sciencedirect.com/science/article/pii/S0022227520325141HDLcholesteryl esterselective uptakelipoprotein lipaseliverreverse cholesterol transport |
| spellingShingle | Franz Rinninger Tatjana Kaiser W. Alexander Mann Nicolette Meyer Heiner Greten Ulrike Beisiegel Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture Journal of Lipid Research HDL cholesteryl ester selective uptake lipoprotein lipase liver reverse cholesterol transport |
| title | Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture |
| title_full | Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture |
| title_fullStr | Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture |
| title_full_unstemmed | Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture |
| title_short | Lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein-associated cholesteryl esters by hepatic cells in culture |
| title_sort | lipoprotein lipase mediates an increase in the selective uptake of high density lipoprotein associated cholesteryl esters by hepatic cells in culture |
| topic | HDL cholesteryl ester selective uptake lipoprotein lipase liver reverse cholesterol transport |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520325141 |
| work_keys_str_mv | AT franzrinninger lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture AT tatjanakaiser lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture AT walexandermann lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture AT nicolettemeyer lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture AT heinergreten lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture AT ulrikebeisiegel lipoproteinlipasemediatesanincreaseintheselectiveuptakeofhighdensitylipoproteinassociatedcholesterylestersbyhepaticcellsinculture |