Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang
Fragaria x ananassa (strawberry) is a subtropical plant that can adapt well in tropical highlands. Fragaria x ananassa have been widely used to cope with health problems. The active compound component of secondary metabolites contained in Fragaria x ananassa has the potential as an antioxidant. This...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2019-12-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/299 |
| _version_ | 1818243082662969344 |
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| author | Desak Gede Sri Andayani Puspa Dewi Narrij Lotulung Anny Sulaswaty Nur Qaanitaati Desak Gede Tirta Andini Rahmaniar Mulyani Eva Nursyifa |
| author_facet | Desak Gede Sri Andayani Puspa Dewi Narrij Lotulung Anny Sulaswaty Nur Qaanitaati Desak Gede Tirta Andini Rahmaniar Mulyani Eva Nursyifa |
| author_sort | Desak Gede Sri Andayani |
| collection | DOAJ |
| description | Fragaria x ananassa (strawberry) is a subtropical plant that can adapt well in tropical highlands. Fragaria x ananassa have been widely used to cope with health problems. The active compound component of secondary metabolites contained in Fragaria x ananassa has the potential as an antioxidant. This research is done to isolate secondary metabolites from extract of Fragaria x ananassa fruits. Extract Fragaria x ananassa was produced by maceration using ethanol as the solvent. Separation and isolation compound were carried out using Vacuum Liquid Chromatography (VLC) and Gravity Column Chromatography (GCC) guided by Thin Layer Chromatography (TLC) using hexane: ethyl acetate (3:7) as the eluent. The flavonoid compound was determined by the total content of phenolic and flavonoid in extract of Fragaria x ananassa fruits. The results of total phenolic content and total flavonoid content were 0.1130 mg/g and 0.0112 mg/g, respectively. The alkaloid compound was determined by Dragendorff testing. The elucidation of the structure by Fourier Tansform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography Mass Spectrometry (LCMS) showed that the active compound contained in the secondary metabolite of extract ethanol from Fragaria x ananassa is 3-Cyclopentyl-5-(1-hydroxyethyl)-1,6-dihydro-7H-pyrazolo[4,3- d]pyrimidin-7-one.
Keywords: Fragaria x ananassa extract, flavonoid, alkaloid, total phenolic and flavonoid content, FTIR, NMR, LCMS. |
| first_indexed | 2024-12-12T13:55:28Z |
| format | Article |
| id | doaj.art-7c50935922924490b57f68c979f4aa8e |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-12-12T13:55:28Z |
| publishDate | 2019-12-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-7c50935922924490b57f68c979f4aa8e2022-12-22T00:22:28ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892019-12-0110315916810.14499/indonesianjcanchemoprev10iss3pp159-168194Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. LembangDesak Gede Sri Andayani0Puspa Dewi Narrij Lotulung1Anny Sulaswaty2Nur Qaanitaati3Desak Gede Tirta Andini4Rahmaniar Mulyani5Eva Nursyifa6Indonesian Institute of Sciences, Research Unit for Clean TechnologyResearch Centre for Chemistry-Indonesian Institute of Sciences, Puspiptek Serpong, TangerangResearch Centre for Chemistry-Indonesian Institute of Sciences, Puspiptek Serpong, TangerangJenderal Achmad Yani University, Bandung West JavaFaculty of Science and Technology, Airlangga University, SurabayaJenderal Achmad Yani University, Bandung West Java,Jenderal Achmad Yani University, Bandung West JavaFragaria x ananassa (strawberry) is a subtropical plant that can adapt well in tropical highlands. Fragaria x ananassa have been widely used to cope with health problems. The active compound component of secondary metabolites contained in Fragaria x ananassa has the potential as an antioxidant. This research is done to isolate secondary metabolites from extract of Fragaria x ananassa fruits. Extract Fragaria x ananassa was produced by maceration using ethanol as the solvent. Separation and isolation compound were carried out using Vacuum Liquid Chromatography (VLC) and Gravity Column Chromatography (GCC) guided by Thin Layer Chromatography (TLC) using hexane: ethyl acetate (3:7) as the eluent. The flavonoid compound was determined by the total content of phenolic and flavonoid in extract of Fragaria x ananassa fruits. The results of total phenolic content and total flavonoid content were 0.1130 mg/g and 0.0112 mg/g, respectively. The alkaloid compound was determined by Dragendorff testing. The elucidation of the structure by Fourier Tansform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography Mass Spectrometry (LCMS) showed that the active compound contained in the secondary metabolite of extract ethanol from Fragaria x ananassa is 3-Cyclopentyl-5-(1-hydroxyethyl)-1,6-dihydro-7H-pyrazolo[4,3- d]pyrimidin-7-one. Keywords: Fragaria x ananassa extract, flavonoid, alkaloid, total phenolic and flavonoid content, FTIR, NMR, LCMS.https://ijcc.chemoprev.org/index.php/ijcc/article/view/299 |
| spellingShingle | Desak Gede Sri Andayani Puspa Dewi Narrij Lotulung Anny Sulaswaty Nur Qaanitaati Desak Gede Tirta Andini Rahmaniar Mulyani Eva Nursyifa Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang |
| title_full | Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang |
| title_fullStr | Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang |
| title_full_unstemmed | Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang |
| title_short | Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang |
| title_sort | revelation of new compound from ethanolic extract of fragaria x ananassa var lembang |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/299 |
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