Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods
Honey bees are threatened by many fungal, parasitic and bacterial diseases. This study was aimed at identifying filamentous fungi colonizing the intestinal region of dead adult honey bees and determining resultant mycotoxin activity and its potential adverse implications for bee and human health by...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-09-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3020 |
| _version_ | 1797403847175438336 |
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| author | Serdal TARHANE İnan DURSUN Baycan MOR Ayşe KANICI TARHANE Şemistan KIZILTEPE İbrahim FİLAZİ Aysel GÜVEN |
| author_facet | Serdal TARHANE İnan DURSUN Baycan MOR Ayşe KANICI TARHANE Şemistan KIZILTEPE İbrahim FİLAZİ Aysel GÜVEN |
| author_sort | Serdal TARHANE |
| collection | DOAJ |
| description | Honey bees are threatened by many fungal, parasitic and bacterial diseases. This
study was aimed at identifying filamentous fungi colonizing the intestinal region of
dead adult honey bees and determining resultant mycotoxin activity and its potential
adverse implications for bee and human health by the PCR and UHPLC-Orbitrap-
HRMS methods. For this purpose, dead bees were collected from the ground in front
of 95 hives displaying mass mortality in the Hatay, Iğdır and Bingöl provinces, and 22
filamentous and 6 yeast-like fungi were isolated and identified from the intestinal region
of these bees. Of the filamentous fungal isolates, 8 were identified as Aspergillus spp.,
3 as Fusarium spp., 2 as Alternaria spp. 5 as Penicillium spp., 4 as Mucor spp., and 2 as
Rhizopus spp.. The PCR analysis of the filamentous fungi using primers targeting the
aflatoxin and ochratoxin A genes revealed the presence of aflatoxin in only 1 out of the
22 samples. Aflatoxin and ochratoxin were not detected in any of the other samples.
The UHPLC-Orbitrap-HRMS method revealed the presence of aflatoxin B1 in 17, both
B1 and B2 in 6, B1, B2 and G1 in 5, and aflatoxin G2 in 1 of the filamentous fungal
samples. The results obtained in the present study suggest that filamentous fungi may
produce mycotoxins in the intestinal region of honey bees, and thereby, honey bees may
distribute mycotoxins into the environment and cause indirect adverse effects on human
and animal health. |
| first_indexed | 2024-03-09T02:44:23Z |
| format | Article |
| id | doaj.art-7c517307a1874bbabaf69547934c9984 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-09T02:44:23Z |
| publishDate | 2023-09-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-7c517307a1874bbabaf69547934c99842023-12-05T21:27:25ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-09-0129547348110.9775/kvfd.2023.294473020Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methodsSerdal TARHANE0İnan DURSUN1Baycan MOR2Ayşe KANICI TARHANE3Şemistan KIZILTEPE4İbrahim FİLAZİ5Aysel GÜVEN6Çankırı Karatekin University, Eldivan Vocational School of Health Services, Department of Veterinary, TR-18000 Çankırı -TÜRKİYEBingöl University, Central Laboratory Application and Research Center /Vocational School of Food Agriculture and Livestock, TR-12000 Bingöl -TÜRKİYEKafkas University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, TR-36000 Kars -TÜRKİYEKafkas University, Faculty of Veterinary Science, Department of Pharmacology and Toxicology, TR-36100 Kars -TÜRKİYEIgdir University, Tuzluca Vocational School, TR-76000 Iğdır -TÜRKİYEÇankırı Karatekin University, Central Laboratory Application and Research Center (ÇANKAM), TR-18000 Çankırı -TÜRKİYEBaskent University, Vocational School of Pathology Laboratory, TR-06790 Ankara –TÜRKİYEHoney bees are threatened by many fungal, parasitic and bacterial diseases. This study was aimed at identifying filamentous fungi colonizing the intestinal region of dead adult honey bees and determining resultant mycotoxin activity and its potential adverse implications for bee and human health by the PCR and UHPLC-Orbitrap- HRMS methods. For this purpose, dead bees were collected from the ground in front of 95 hives displaying mass mortality in the Hatay, Iğdır and Bingöl provinces, and 22 filamentous and 6 yeast-like fungi were isolated and identified from the intestinal region of these bees. Of the filamentous fungal isolates, 8 were identified as Aspergillus spp., 3 as Fusarium spp., 2 as Alternaria spp. 5 as Penicillium spp., 4 as Mucor spp., and 2 as Rhizopus spp.. The PCR analysis of the filamentous fungi using primers targeting the aflatoxin and ochratoxin A genes revealed the presence of aflatoxin in only 1 out of the 22 samples. Aflatoxin and ochratoxin were not detected in any of the other samples. The UHPLC-Orbitrap-HRMS method revealed the presence of aflatoxin B1 in 17, both B1 and B2 in 6, B1, B2 and G1 in 5, and aflatoxin G2 in 1 of the filamentous fungal samples. The results obtained in the present study suggest that filamentous fungi may produce mycotoxins in the intestinal region of honey bees, and thereby, honey bees may distribute mycotoxins into the environment and cause indirect adverse effects on human and animal health.https://vetdergikafkas.org/pdf.php?id=3020filamentous fungushoney beemycotoxin |
| spellingShingle | Serdal TARHANE İnan DURSUN Baycan MOR Ayşe KANICI TARHANE Şemistan KIZILTEPE İbrahim FİLAZİ Aysel GÜVEN Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods Kafkas Universitesi Veteriner Fakültesi Dergisi filamentous fungus honey bee mycotoxin |
| title | Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods |
| title_full | Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods |
| title_fullStr | Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods |
| title_full_unstemmed | Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods |
| title_short | Determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap- hrms methods |
| title_sort | determination of the mycotoxin activity of filamentous fungi isolated from the intestinal region of adult honey bees by the pcr and uhplcorbitrap hrms methods |
| topic | filamentous fungus honey bee mycotoxin |
| url | https://vetdergikafkas.org/pdf.php?id=3020 |
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