Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity
Abstract The main attempt of this study is to isolate, determine potential probiotic properties and enzyme production of some lactic acid bacteria (LAB). Among all isolates, two LAB strains isolated from human mother milk and cottage cheese revealed antimicrobial activity against some tested pathoge...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-27003-2 |
| _version_ | 1797973638882787328 |
|---|---|
| author | Abeer I. M. EL-Sayed Aliaa M. El-Borai Sara H. Akl Samy A. EL-Aassar Mohamed S. Abdel-Latif |
| author_facet | Abeer I. M. EL-Sayed Aliaa M. El-Borai Sara H. Akl Samy A. EL-Aassar Mohamed S. Abdel-Latif |
| author_sort | Abeer I. M. EL-Sayed |
| collection | DOAJ |
| description | Abstract The main attempt of this study is to isolate, determine potential probiotic properties and enzyme production of some lactic acid bacteria (LAB). Among all isolates, two LAB strains isolated from human mother milk and cottage cheese revealed antimicrobial activity against some tested pathogenic strains. Both isolates inhibited all the tested pathogens except Escherichia coli. The two isolates were identified by morphological, biochemical properties and then by 16S rRNA gene sequencing technique as Lactobacillus acidophilus SAM1 and Lactiplantibacillus plantarum SAM2. Potential probiotic characters were investigated. Both strains survived in relatively low pH and high bile concentrations and were able to grow at 0.5% of pancreatin concentrations. Their growth decreased by increasing phenol from 0.2% till 0.5%. Both strains did not show hemolytic activity. Coaggregation potential was exhibited by the two strains against Staphylococcus aureus and Candida albicans. Hydrophobicity of Lactobacillus acidophilus SAM1 and Lactiplantibacillus plantarum SAM2, with ethyl acetate; were 88.1% and 82.8%, respectively. Lactobacillus acidophilus SAM1 was susceptible to Ampicillin, Penicillin, Erythromycin, Ciprofloxacin and Tetracycline; on the contrary, it resists Vancomycin and Cefoxitin; while Lactiplantibacillus plantarum SAM2 resists all tested antibiotics. Maximum growth was achieved using glucose as a carbon source and yeast extract as nitrogen source for both strains; however, glucose is the most preferred carbon source for microorganisms and it prevents the uptake of carbon from other sources like yeast by catabolite repression mechanism. Lactobacillus acidophilus SAM1 produces lipase enzyme, while Lactiplantibacillus plantarum SAM2 produces amylase and protease. |
| first_indexed | 2024-04-11T04:07:27Z |
| format | Article |
| id | doaj.art-7c54c657316e446fa1617562e3f322e9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T04:07:27Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7c54c657316e446fa1617562e3f322e92023-01-01T12:17:37ZengNature PortfolioScientific Reports2045-23222022-12-0112111310.1038/s41598-022-27003-2Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activityAbeer I. M. EL-Sayed0Aliaa M. El-Borai1Sara H. Akl2Samy A. EL-Aassar3Mohamed S. Abdel-Latif4Botany and Microbiology Department, Faculty of Science, Damanhour UniversityBotany and Microbiology Department, Faculty of Science, Alexandria UniversityMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in AlexandriaBotany and Microbiology Department, Faculty of Science, Alexandria UniversityMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in AlexandriaAbstract The main attempt of this study is to isolate, determine potential probiotic properties and enzyme production of some lactic acid bacteria (LAB). Among all isolates, two LAB strains isolated from human mother milk and cottage cheese revealed antimicrobial activity against some tested pathogenic strains. Both isolates inhibited all the tested pathogens except Escherichia coli. The two isolates were identified by morphological, biochemical properties and then by 16S rRNA gene sequencing technique as Lactobacillus acidophilus SAM1 and Lactiplantibacillus plantarum SAM2. Potential probiotic characters were investigated. Both strains survived in relatively low pH and high bile concentrations and were able to grow at 0.5% of pancreatin concentrations. Their growth decreased by increasing phenol from 0.2% till 0.5%. Both strains did not show hemolytic activity. Coaggregation potential was exhibited by the two strains against Staphylococcus aureus and Candida albicans. Hydrophobicity of Lactobacillus acidophilus SAM1 and Lactiplantibacillus plantarum SAM2, with ethyl acetate; were 88.1% and 82.8%, respectively. Lactobacillus acidophilus SAM1 was susceptible to Ampicillin, Penicillin, Erythromycin, Ciprofloxacin and Tetracycline; on the contrary, it resists Vancomycin and Cefoxitin; while Lactiplantibacillus plantarum SAM2 resists all tested antibiotics. Maximum growth was achieved using glucose as a carbon source and yeast extract as nitrogen source for both strains; however, glucose is the most preferred carbon source for microorganisms and it prevents the uptake of carbon from other sources like yeast by catabolite repression mechanism. Lactobacillus acidophilus SAM1 produces lipase enzyme, while Lactiplantibacillus plantarum SAM2 produces amylase and protease.https://doi.org/10.1038/s41598-022-27003-2 |
| spellingShingle | Abeer I. M. EL-Sayed Aliaa M. El-Borai Sara H. Akl Samy A. EL-Aassar Mohamed S. Abdel-Latif Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity Scientific Reports |
| title | Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| title_full | Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| title_fullStr | Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| title_full_unstemmed | Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| title_short | Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| title_sort | identification of lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity |
| url | https://doi.org/10.1038/s41598-022-27003-2 |
| work_keys_str_mv | AT abeerimelsayed identificationoflactobacillusstrainsfromhumanmothermilkandcottagecheeserevealedpotentialprobioticpropertieswithenzymaticactivity AT aliaamelborai identificationoflactobacillusstrainsfromhumanmothermilkandcottagecheeserevealedpotentialprobioticpropertieswithenzymaticactivity AT sarahakl identificationoflactobacillusstrainsfromhumanmothermilkandcottagecheeserevealedpotentialprobioticpropertieswithenzymaticactivity AT samyaelaassar identificationoflactobacillusstrainsfromhumanmothermilkandcottagecheeserevealedpotentialprobioticpropertieswithenzymaticactivity AT mohamedsabdellatif identificationoflactobacillusstrainsfromhumanmothermilkandcottagecheeserevealedpotentialprobioticpropertieswithenzymaticactivity |