In Vitro Propagation of Groundnut (Arachis hypogea L.)
A protocol has been developed for micropropagation of Arachis hypogea L. under in vitro conditions. Nodal explants gave rise to multiple shoots when cultured on MS medium supplemented with different concentrations of BA (benzyladenine) with Kin (Kinetin) or GA3 (gibberellic acid). The highest res...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2017-05-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/561 |
| _version_ | 1818192173350256640 |
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| author | Kh. R. Al-Joboury |
| author_facet | Kh. R. Al-Joboury |
| author_sort | Kh. R. Al-Joboury |
| collection | DOAJ |
| description |
A protocol has been developed for micropropagation of Arachis hypogea L. under in
vitro conditions. Nodal explants gave rise to multiple shoots when cultured on MS medium
supplemented with different concentrations of BA (benzyladenine) with Kin (Kinetin) or GA3
(gibberellic acid). The highest response of shoot multiplication was obtained in MS
containing 1.5 mg.l¯¹ BA and 0.5 mg.L¯¹ Kin. The regenerated shootlets were rooted on MS
(Murashige and Skoog) basal medium with different concentrations of IBA (indolbutyric
acid) and IAA (indol acetic acid). The highest response of rooting was achieved with IBA at
0.05 + IAA at 0.05 mg.L¯¹. The maximum frequency of rooting and highest number of roots
were produced on medium containing IBA 0.05 mg.L¯¹ and IAA 0.05 mg.L¯¹. The plantlets,
thus successfully established in soil.
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| first_indexed | 2024-12-12T00:26:17Z |
| format | Article |
| id | doaj.art-7c56c30dd1f04042a1a3e1922ed1a969 |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-12-12T00:26:17Z |
| publishDate | 2017-05-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-7c56c30dd1f04042a1a3e1922ed1a9692022-12-22T00:44:37ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072017-05-01253In Vitro Propagation of Groundnut (Arachis hypogea L.)Kh. R. Al-Joboury A protocol has been developed for micropropagation of Arachis hypogea L. under in vitro conditions. Nodal explants gave rise to multiple shoots when cultured on MS medium supplemented with different concentrations of BA (benzyladenine) with Kin (Kinetin) or GA3 (gibberellic acid). The highest response of shoot multiplication was obtained in MS containing 1.5 mg.l¯¹ BA and 0.5 mg.L¯¹ Kin. The regenerated shootlets were rooted on MS (Murashige and Skoog) basal medium with different concentrations of IBA (indolbutyric acid) and IAA (indol acetic acid). The highest response of rooting was achieved with IBA at 0.05 + IAA at 0.05 mg.L¯¹. The maximum frequency of rooting and highest number of roots were produced on medium containing IBA 0.05 mg.L¯¹ and IAA 0.05 mg.L¯¹. The plantlets, thus successfully established in soil. https://jih.uobaghdad.edu.iq/index.php/j/article/view/561Arachis hypogea, tissue culture, in vitro, plant regeneration. |
| spellingShingle | Kh. R. Al-Joboury In Vitro Propagation of Groundnut (Arachis hypogea L.) Ibn Al-Haitham Journal for Pure and Applied Sciences Arachis hypogea, tissue culture, in vitro, plant regeneration. |
| title | In Vitro Propagation of Groundnut (Arachis hypogea L.) |
| title_full | In Vitro Propagation of Groundnut (Arachis hypogea L.) |
| title_fullStr | In Vitro Propagation of Groundnut (Arachis hypogea L.) |
| title_full_unstemmed | In Vitro Propagation of Groundnut (Arachis hypogea L.) |
| title_short | In Vitro Propagation of Groundnut (Arachis hypogea L.) |
| title_sort | in vitro propagation of groundnut arachis hypogea l |
| topic | Arachis hypogea, tissue culture, in vitro, plant regeneration. |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/561 |
| work_keys_str_mv | AT khraljoboury invitropropagationofgroundnutarachishypogeal |