Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures
Summary: Secretory pathways within dendrites of neurons have been proposed for local transport of newly synthesized proteins. However, little is known about the dynamics of the local secretory system and whether the organelles are transient or stable structures. Here, we quantify the spatial and dyn...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723007209 |
| _version_ | 1827911162747944960 |
|---|---|
| author | Jingqi Wang Maciej Daniszewski Marlene M. Hao Damián Hernández Alice Pébay Paul A. Gleeson Lou Fourriere |
| author_facet | Jingqi Wang Maciej Daniszewski Marlene M. Hao Damián Hernández Alice Pébay Paul A. Gleeson Lou Fourriere |
| author_sort | Jingqi Wang |
| collection | DOAJ |
| description | Summary: Secretory pathways within dendrites of neurons have been proposed for local transport of newly synthesized proteins. However, little is known about the dynamics of the local secretory system and whether the organelles are transient or stable structures. Here, we quantify the spatial and dynamic behavior of dendritic Golgi and endosomes during differentiation of human neurons generated from induced pluripotent stem cells (iPSCs). In early neuronal development, before and during migration, the entire Golgi apparatus transiently translocates from the soma into dendrites. In mature neurons, dynamic Golgi elements, containing cis and trans cisternae, are transported from the soma along dendrites, in an actin-dependent process. Dendritic Golgi outposts are dynamic and display bidirectional movement. Similar structures were observed in cerebral organoids. Using the retention using selective hooks (RUSH) system, Golgi resident proteins are transported efficiently into Golgi outposts from the endoplasmic reticulum. This study reveals dynamic, functional Golgi structures in dendrites and a spatial map for investigating dendrite trafficking in human neurons. |
| first_indexed | 2024-03-13T01:59:25Z |
| format | Article |
| id | doaj.art-7c5800b8066d4d5a9238cde3018867d7 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-13T01:59:25Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-7c5800b8066d4d5a9238cde3018867d72023-07-02T04:16:37ZengElsevierCell Reports2211-12472023-07-01427112709Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structuresJingqi Wang0Maciej Daniszewski1Marlene M. Hao2Damián Hernández3Alice Pébay4Paul A. Gleeson5Lou Fourriere6The Department of Biochemistry and Pharmacology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, VIC 3010, Australia; Department of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC 3010, AustraliaThe Department of Biochemistry and Pharmacology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia; Corresponding authorThe Department of Biochemistry and Pharmacology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia; Corresponding authorSummary: Secretory pathways within dendrites of neurons have been proposed for local transport of newly synthesized proteins. However, little is known about the dynamics of the local secretory system and whether the organelles are transient or stable structures. Here, we quantify the spatial and dynamic behavior of dendritic Golgi and endosomes during differentiation of human neurons generated from induced pluripotent stem cells (iPSCs). In early neuronal development, before and during migration, the entire Golgi apparatus transiently translocates from the soma into dendrites. In mature neurons, dynamic Golgi elements, containing cis and trans cisternae, are transported from the soma along dendrites, in an actin-dependent process. Dendritic Golgi outposts are dynamic and display bidirectional movement. Similar structures were observed in cerebral organoids. Using the retention using selective hooks (RUSH) system, Golgi resident proteins are transported efficiently into Golgi outposts from the endoplasmic reticulum. This study reveals dynamic, functional Golgi structures in dendrites and a spatial map for investigating dendrite trafficking in human neurons.http://www.sciencedirect.com/science/article/pii/S2211124723007209CP: Cell biologyCP: Neuroscience |
| spellingShingle | Jingqi Wang Maciej Daniszewski Marlene M. Hao Damián Hernández Alice Pébay Paul A. Gleeson Lou Fourriere Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures Cell Reports CP: Cell biology CP: Neuroscience |
| title | Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures |
| title_full | Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures |
| title_fullStr | Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures |
| title_full_unstemmed | Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures |
| title_short | Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures |
| title_sort | organelle mapping in dendrites of human ipsc derived neurons reveals dynamic functional dendritic golgi structures |
| topic | CP: Cell biology CP: Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723007209 |
| work_keys_str_mv | AT jingqiwang organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT maciejdaniszewski organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT marlenemhao organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT damianhernandez organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT alicepebay organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT paulagleeson organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures AT loufourriere organellemappingindendritesofhumanipscderivedneuronsrevealsdynamicfunctionaldendriticgolgistructures |