Cell Migration and Invasion Assays as Tools for Drug Discovery
Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologicall...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-03-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4923/3/1/107/ |
| _version_ | 1811278181238308864 |
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| author | Keren I. Hulkower Renee L. Herber |
| author_facet | Keren I. Hulkower Renee L. Herber |
| author_sort | Keren I. Hulkower |
| collection | DOAJ |
| description | Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays. |
| first_indexed | 2024-04-13T00:30:02Z |
| format | Article |
| id | doaj.art-7c5b0baee19d4e7f9bf736294778385e |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-13T00:30:02Z |
| publishDate | 2011-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7c5b0baee19d4e7f9bf736294778385e2022-12-22T03:10:29ZengMDPI AGPharmaceutics1999-49232011-03-013110712410.3390/pharmaceutics3010107Cell Migration and Invasion Assays as Tools for Drug DiscoveryKeren I. HulkowerRenee L. HerberCell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.http://www.mdpi.com/1999-4923/3/1/107/cell migrationcell invasionhigh throughput screeninghigh content analysisphenotypic assaysdrug discovery3-dimensional assayswound healingcancer metastasis |
| spellingShingle | Keren I. Hulkower Renee L. Herber Cell Migration and Invasion Assays as Tools for Drug Discovery Pharmaceutics cell migration cell invasion high throughput screening high content analysis phenotypic assays drug discovery 3-dimensional assays wound healing cancer metastasis |
| title | Cell Migration and Invasion Assays as Tools for Drug Discovery |
| title_full | Cell Migration and Invasion Assays as Tools for Drug Discovery |
| title_fullStr | Cell Migration and Invasion Assays as Tools for Drug Discovery |
| title_full_unstemmed | Cell Migration and Invasion Assays as Tools for Drug Discovery |
| title_short | Cell Migration and Invasion Assays as Tools for Drug Discovery |
| title_sort | cell migration and invasion assays as tools for drug discovery |
| topic | cell migration cell invasion high throughput screening high content analysis phenotypic assays drug discovery 3-dimensional assays wound healing cancer metastasis |
| url | http://www.mdpi.com/1999-4923/3/1/107/ |
| work_keys_str_mv | AT kerenihulkower cellmigrationandinvasionassaysastoolsfordrugdiscovery AT reneelherber cellmigrationandinvasionassaysastoolsfordrugdiscovery |