Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.
Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excell...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22911853/?tool=EBI |
| _version_ | 1818700863536889856 |
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| author | Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki |
| author_facet | Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki |
| author_sort | Sebastian Foersch |
| collection | DOAJ |
| description | Early detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Aim of the current study was to evaluate CLE for in vivo diagnosis in different types and models of intracranial neoplasia. In vivo histomorphology of healthy brains and two different C6 glioma cell line allografts was evaluated in rats. One cell line expressed EYFP, the other cell line was used for staining with fluorescent dyes (fluorescein, acriflavine, FITC-dextran and Indocyanine green). To evaluate future application in patients, fresh surgical resection specimen of human intracranial tumors (n = 15) were examined (glioblastoma multiforme, meningioma, craniopharyngioma, acoustic neurinoma, brain metastasis, medulloblastoma, epidermoid tumor). Healthy brain tissue adjacent to the samples served as control. CLE yielded high-quality histomorphology of normal brain tissue and tumors. Different fluorescent agents revealed distinct aspects of tissue and cell structure (nuclear pattern, axonal pathways, hemorrhages). CLE discrimination of neoplastic from healthy brain tissue was easy to perform based on tissue and cellular architecture and resemblance with histopathology was excellent. Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. The technology might be transferred to scientific and clinical application in neurosurgery and neuropathology. It may become helpful to screen for tumor free margins and to improve the surgical resection of malignant brain tumors, and opens the door to in vivo molecular imaging of tumors and other neurologic disorders. |
| first_indexed | 2024-12-17T15:11:42Z |
| format | Article |
| id | doaj.art-7c5d3efcd3c0450a82a93a508032dd36 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T15:11:42Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7c5d3efcd3c0450a82a93a508032dd362022-12-21T21:43:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4176010.1371/journal.pone.0041760Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo.Sebastian FoerschAxel HeimannAli AyyadGilles A SpodenLuise FlorinKonstantin MpoukouvalasRalf KiesslichOliver KempskiMartin GoetzPatra CharalampakiEarly detection and evaluation of brain tumors during surgery is crucial for accurate resection. Currently cryosections during surgery are regularly performed. Confocal laser endomicroscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Aim of the current study was to evaluate CLE for in vivo diagnosis in different types and models of intracranial neoplasia. In vivo histomorphology of healthy brains and two different C6 glioma cell line allografts was evaluated in rats. One cell line expressed EYFP, the other cell line was used for staining with fluorescent dyes (fluorescein, acriflavine, FITC-dextran and Indocyanine green). To evaluate future application in patients, fresh surgical resection specimen of human intracranial tumors (n = 15) were examined (glioblastoma multiforme, meningioma, craniopharyngioma, acoustic neurinoma, brain metastasis, medulloblastoma, epidermoid tumor). Healthy brain tissue adjacent to the samples served as control. CLE yielded high-quality histomorphology of normal brain tissue and tumors. Different fluorescent agents revealed distinct aspects of tissue and cell structure (nuclear pattern, axonal pathways, hemorrhages). CLE discrimination of neoplastic from healthy brain tissue was easy to perform based on tissue and cellular architecture and resemblance with histopathology was excellent. Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. The technology might be transferred to scientific and clinical application in neurosurgery and neuropathology. It may become helpful to screen for tumor free margins and to improve the surgical resection of malignant brain tumors, and opens the door to in vivo molecular imaging of tumors and other neurologic disorders.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22911853/?tool=EBI |
| spellingShingle | Sebastian Foersch Axel Heimann Ali Ayyad Gilles A Spoden Luise Florin Konstantin Mpoukouvalas Ralf Kiesslich Oliver Kempski Martin Goetz Patra Charalampaki Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. PLoS ONE |
| title | Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. |
| title_full | Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. |
| title_fullStr | Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. |
| title_full_unstemmed | Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. |
| title_short | Confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo. |
| title_sort | confocal laser endomicroscopy for diagnosis and histomorphologic imaging of brain tumors in vivo |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22911853/?tool=EBI |
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