Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides
Various proteins with antibacterial, anticoagulant, and anti-inflammatory properties have been identified in the buccal glands of jawless blood-sucking vertebrate lampreys. However, studies on endogenous peptides in the buccal gland of lampreys are limited. In this study, 4528 endogenous peptides we...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/7/389 |
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| author | Yaocen Wang Feng Sun Zhuoying Wang Xuyuan Duan Qingwei Li Yue Pang Meng Gou |
| author_facet | Yaocen Wang Feng Sun Zhuoying Wang Xuyuan Duan Qingwei Li Yue Pang Meng Gou |
| author_sort | Yaocen Wang |
| collection | DOAJ |
| description | Various proteins with antibacterial, anticoagulant, and anti-inflammatory properties have been identified in the buccal glands of jawless blood-sucking vertebrate lampreys. However, studies on endogenous peptides in the buccal gland of lampreys are limited. In this study, 4528 endogenous peptides were identified from 1224 precursor proteins using peptidomics and screened for bioactivity in the buccal glands of the lamprey, <i>Lethenteron camtschaticum</i>. We synthesized four candidate bioactive peptides (VSLNLPYSVVRGEQFVVQA, DIPVPEVPILE, VVQLPPVVLGTFG, and VPPPPLVLPPASVK), calculated their secondary structures, and validated their bioactivity. The results showed that the peptide VSLNLPYSVVRGEQFVVQA possessed anti-inflammatory activity, which significantly increased the expression of anti-inflammatory factors and decreased the expression of inflammatory factors in THP-1 cells. The peptide VVQLPPVVLGTFG showed antibacterial activity against some gram-positive bacteria. The peptide VSLNLPYSVVRGEQFVQA possessed good ACE inhibitory activity at low concentrations, but no dose-related correlation was observed. Our study revealed that the buccal glands of the jawless vertebrate lamprey are a source of multiple bioactive peptides, which will provide new insights into the blood-sucking mechanism of lamprey. |
| first_indexed | 2024-03-11T00:52:55Z |
| format | Article |
| id | doaj.art-7c5d7f0916894bf6a52d7571df4066a2 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T00:52:55Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-7c5d7f0916894bf6a52d7571df4066a22023-11-18T20:14:06ZengMDPI AGMarine Drugs1660-33972023-06-0121738910.3390/md21070389Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive PeptidesYaocen Wang0Feng Sun1Zhuoying Wang2Xuyuan Duan3Qingwei Li4Yue Pang5Meng Gou6College of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaCollege of Life Science, Liaoning Normal University, Dalian 116081, ChinaVarious proteins with antibacterial, anticoagulant, and anti-inflammatory properties have been identified in the buccal glands of jawless blood-sucking vertebrate lampreys. However, studies on endogenous peptides in the buccal gland of lampreys are limited. In this study, 4528 endogenous peptides were identified from 1224 precursor proteins using peptidomics and screened for bioactivity in the buccal glands of the lamprey, <i>Lethenteron camtschaticum</i>. We synthesized four candidate bioactive peptides (VSLNLPYSVVRGEQFVVQA, DIPVPEVPILE, VVQLPPVVLGTFG, and VPPPPLVLPPASVK), calculated their secondary structures, and validated their bioactivity. The results showed that the peptide VSLNLPYSVVRGEQFVVQA possessed anti-inflammatory activity, which significantly increased the expression of anti-inflammatory factors and decreased the expression of inflammatory factors in THP-1 cells. The peptide VVQLPPVVLGTFG showed antibacterial activity against some gram-positive bacteria. The peptide VSLNLPYSVVRGEQFVQA possessed good ACE inhibitory activity at low concentrations, but no dose-related correlation was observed. Our study revealed that the buccal glands of the jawless vertebrate lamprey are a source of multiple bioactive peptides, which will provide new insights into the blood-sucking mechanism of lamprey.https://www.mdpi.com/1660-3397/21/7/389lampreypeptidomicsantimicrobialanti-inflammatoryendogenous peptides |
| spellingShingle | Yaocen Wang Feng Sun Zhuoying Wang Xuyuan Duan Qingwei Li Yue Pang Meng Gou Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides Marine Drugs lamprey peptidomics antimicrobial anti-inflammatory endogenous peptides |
| title | Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides |
| title_full | Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides |
| title_fullStr | Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides |
| title_full_unstemmed | Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides |
| title_short | Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides |
| title_sort | peptidomics analysis reveals the buccal gland of jawless vertebrate lamprey as a source of multiple bioactive peptides |
| topic | lamprey peptidomics antimicrobial anti-inflammatory endogenous peptides |
| url | https://www.mdpi.com/1660-3397/21/7/389 |
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