Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms
Bacteria in planktonic and biofilm forms exhibit different phenotypic properties. In this study, the phenotypic traits and probiotic functions of <i>Lactiplantibacillus plantarum</i> Y42 in planktonic and biofilm forms were assessed. After 36 h of static culture, scanning electron micros...
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1516 |
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| author | Jiayi Li Guangqing Mu Yanfeng Tuo |
| author_facet | Jiayi Li Guangqing Mu Yanfeng Tuo |
| author_sort | Jiayi Li |
| collection | DOAJ |
| description | Bacteria in planktonic and biofilm forms exhibit different phenotypic properties. In this study, the phenotypic traits and probiotic functions of <i>Lactiplantibacillus plantarum</i> Y42 in planktonic and biofilm forms were assessed. After 36 h of static culture, scanning electron microscopy and confocal laser scanning microscopy showed that the <i>L</i>. <i>plantarum</i> Y42 bacterial cells contained interconnected adhesive matter on the surface, forming a ~18 μm layer of dense biofilms. The surface properties of <i>L</i>. <i>plantarum</i> Y42 in biofilm form, including autoaggregation ability, hydrophobicity, acid-base charge, and adhesiveness, were all higher than those in the planktonic form. Biofilm <i>L</i>. <i>plantarum</i> Y42 showed a higher tolerance to adverse environmental conditions and a higher survival rate, enzymatic activity, and integrity after vacuum lyophilization. And biofilm <i>L</i>. <i>plantarum</i> Y42 had higher adhesion to human enterocyte HT-29 cell monolayers, inhibited the expressions of proinflammatory factors IL-6 and TNF-α, and promoted the expressions of the anti-inflammatory factor IL-10 and barrier proteins Claudin-1 and Occludin. In addition, <i>L</i>. <i>plantarum</i> Y42 in biofilm form can inhibit the adhesion and invasion of <i>Listeria monocytogenes</i> ATCC 19115 to HT-29 cell monolayers and is more effective in relieving the inflammatory reactions and injuries of HT-29 cells caused by <i>L. monocytogenes</i> ATCC 19115. In conclusion, <i>L</i>. <i>plantarum</i> Y42 in biofilm form exhibited better probiotic functions compared to that in planktonic form. This indicated that <i>L. plantarum</i> Y42 can form biofilms to enhance its probiotic functions, which provided a theoretical basis for better development and utilization of <i>L. plantarum</i> Y42. |
| first_indexed | 2024-03-11T05:36:55Z |
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| spelling | doaj.art-7c5df2292a504b80b865bb387880bbac2023-11-17T16:42:23ZengMDPI AGFoods2304-81582023-04-01127151610.3390/foods12071516Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm FormsJiayi Li0Guangqing Mu1Yanfeng Tuo2School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaBacteria in planktonic and biofilm forms exhibit different phenotypic properties. In this study, the phenotypic traits and probiotic functions of <i>Lactiplantibacillus plantarum</i> Y42 in planktonic and biofilm forms were assessed. After 36 h of static culture, scanning electron microscopy and confocal laser scanning microscopy showed that the <i>L</i>. <i>plantarum</i> Y42 bacterial cells contained interconnected adhesive matter on the surface, forming a ~18 μm layer of dense biofilms. The surface properties of <i>L</i>. <i>plantarum</i> Y42 in biofilm form, including autoaggregation ability, hydrophobicity, acid-base charge, and adhesiveness, were all higher than those in the planktonic form. Biofilm <i>L</i>. <i>plantarum</i> Y42 showed a higher tolerance to adverse environmental conditions and a higher survival rate, enzymatic activity, and integrity after vacuum lyophilization. And biofilm <i>L</i>. <i>plantarum</i> Y42 had higher adhesion to human enterocyte HT-29 cell monolayers, inhibited the expressions of proinflammatory factors IL-6 and TNF-α, and promoted the expressions of the anti-inflammatory factor IL-10 and barrier proteins Claudin-1 and Occludin. In addition, <i>L</i>. <i>plantarum</i> Y42 in biofilm form can inhibit the adhesion and invasion of <i>Listeria monocytogenes</i> ATCC 19115 to HT-29 cell monolayers and is more effective in relieving the inflammatory reactions and injuries of HT-29 cells caused by <i>L. monocytogenes</i> ATCC 19115. In conclusion, <i>L</i>. <i>plantarum</i> Y42 in biofilm form exhibited better probiotic functions compared to that in planktonic form. This indicated that <i>L. plantarum</i> Y42 can form biofilms to enhance its probiotic functions, which provided a theoretical basis for better development and utilization of <i>L. plantarum</i> Y42.https://www.mdpi.com/2304-8158/12/7/1516<i>Lactiplantibacillus plantarum</i> Y42planktonicbiofilmphenotypic traitsprobiotic functions |
| spellingShingle | Jiayi Li Guangqing Mu Yanfeng Tuo Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms Foods <i>Lactiplantibacillus plantarum</i> Y42 planktonic biofilm phenotypic traits probiotic functions |
| title | Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms |
| title_full | Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms |
| title_fullStr | Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms |
| title_full_unstemmed | Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms |
| title_short | Phenotypic Traits and Probiotic Functions of <i>Lactiplantibacillus plantarum</i> Y42 in Planktonic and Biofilm Forms |
| title_sort | phenotypic traits and probiotic functions of i lactiplantibacillus plantarum i y42 in planktonic and biofilm forms |
| topic | <i>Lactiplantibacillus plantarum</i> Y42 planktonic biofilm phenotypic traits probiotic functions |
| url | https://www.mdpi.com/2304-8158/12/7/1516 |
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