Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis
Brucellosis is widespread among humans and animals. Diagnosis of brucellosis mostly depends on serological methods. Serological tests are preferred over time-consuming and hazardous bacterial cultures in routine laboratory practice. However, these tests are somehow challenging due to ‘incomplete/blo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2016-09-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1190945 |
| _version_ | 1818287037193650176 |
|---|---|
| author | Mehmet Koroglu Ozlem Akkaya Aydemir Tayfur Demiray Unal Erkorkmaz Ahmet Ozbek Mustafa Altindis |
| author_facet | Mehmet Koroglu Ozlem Akkaya Aydemir Tayfur Demiray Unal Erkorkmaz Ahmet Ozbek Mustafa Altindis |
| author_sort | Mehmet Koroglu |
| collection | DOAJ |
| description | Brucellosis is widespread among humans and animals. Diagnosis of brucellosis mostly depends on serological methods. Serological tests are preferred over time-consuming and hazardous bacterial cultures in routine laboratory practice. However, these tests are somehow challenging due to ‘incomplete/blocking antibodies’ that prevent agglutination. Brucella Coombs gel test (BCGT) is newly developed test that contains Coombs antibodies (anti-human IgG) in gel microtubes and depends on gel centrifugation methods for the serological diagnosis of brucellosis. Here, performance of the BCGT is compared with standard serum tube agglutination (STA), standard serum tube agglutination with Coombs (C-STA) and immune capture agglutination (Brucellacapt). In total, 78 positive samples for study group and 16 samples for the control group were enrolled in the study. The samples were tested at dilutions of 1:40–1:5120. Titres at 1:160 and above were considered positive for brucellosis, whereas those lower than 1:160 were considered negative. Excellent agreement levels were determined between BCGT test and C-STA (κ, 0.894; p < 0.001), and BCGT and Brucellacapt (κ, 0.802; p < 0.001), when the diagnostic titre was accepted as 1:160. BCGT is easy to apply and interpret and provides reliable titre results in less than 2 h. It is also advantageous for screening. |
| first_indexed | 2024-12-13T01:34:07Z |
| format | Article |
| id | doaj.art-7c5ef9f412b14e6d92b203806f9d890e |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-13T01:34:07Z |
| publishDate | 2016-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-7c5ef9f412b14e6d92b203806f9d890e2022-12-22T00:03:56ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-09-0130597097510.1080/13102818.2016.11909451190945Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosisMehmet Koroglu0Ozlem Akkaya Aydemir1Tayfur Demiray2Unal Erkorkmaz3Ahmet Ozbek4Mustafa Altindis5Sakarya UniversitySakarya University Research and Education HospitalSakarya University Research and Education HospitalSakarya UniversitySakarya UniversitySakarya UniversityBrucellosis is widespread among humans and animals. Diagnosis of brucellosis mostly depends on serological methods. Serological tests are preferred over time-consuming and hazardous bacterial cultures in routine laboratory practice. However, these tests are somehow challenging due to ‘incomplete/blocking antibodies’ that prevent agglutination. Brucella Coombs gel test (BCGT) is newly developed test that contains Coombs antibodies (anti-human IgG) in gel microtubes and depends on gel centrifugation methods for the serological diagnosis of brucellosis. Here, performance of the BCGT is compared with standard serum tube agglutination (STA), standard serum tube agglutination with Coombs (C-STA) and immune capture agglutination (Brucellacapt). In total, 78 positive samples for study group and 16 samples for the control group were enrolled in the study. The samples were tested at dilutions of 1:40–1:5120. Titres at 1:160 and above were considered positive for brucellosis, whereas those lower than 1:160 were considered negative. Excellent agreement levels were determined between BCGT test and C-STA (κ, 0.894; p < 0.001), and BCGT and Brucellacapt (κ, 0.802; p < 0.001), when the diagnostic titre was accepted as 1:160. BCGT is easy to apply and interpret and provides reliable titre results in less than 2 h. It is also advantageous for screening.http://dx.doi.org/10.1080/13102818.2016.1190945Bacterial infectiondiagnosisequipmentbrucellosis |
| spellingShingle | Mehmet Koroglu Ozlem Akkaya Aydemir Tayfur Demiray Unal Erkorkmaz Ahmet Ozbek Mustafa Altindis Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis Biotechnology & Biotechnological Equipment Bacterial infection diagnosis equipment brucellosis |
| title | Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis |
| title_full | Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis |
| title_fullStr | Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis |
| title_full_unstemmed | Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis |
| title_short | Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis |
| title_sort | comparative evaluation of the brucella coombs gel test in laboratory diagnosis of human brucellosis |
| topic | Bacterial infection diagnosis equipment brucellosis |
| url | http://dx.doi.org/10.1080/13102818.2016.1190945 |
| work_keys_str_mv | AT mehmetkoroglu comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis AT ozlemakkayaaydemir comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis AT tayfurdemiray comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis AT unalerkorkmaz comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis AT ahmetozbek comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis AT mustafaaltindis comparativeevaluationofthebrucellacoombsgeltestinlaboratorydiagnosisofhumanbrucellosis |