Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System
Chiral amines are key building blocks for pharmaceuticals. Economic assessment of commercial potential of bioprocesses is needed for guiding research. Biosynthesis of (S)-α-methylbenzylamine (MBA) was selected as case study. For transamination route, transaminase coupled with glucose dehydrogenase a...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2073-4344/10/12/1451 |
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| author | Heyu Huo Guangxiao Yao Shizhen Wang |
| author_facet | Heyu Huo Guangxiao Yao Shizhen Wang |
| author_sort | Heyu Huo |
| collection | DOAJ |
| description | Chiral amines are key building blocks for pharmaceuticals. Economic assessment of commercial potential of bioprocesses is needed for guiding research. Biosynthesis of (S)-α-methylbenzylamine (MBA) was selected as case study. For transamination route, transaminase coupled with glucose dehydrogenase and lactate dehydrogenase catalyzed the reaction with NADH (Nicotinamide adenine dinucleotide) regeneration. Amine dehydrogenase coupled with NADH oxidase, which catalyzed the reductive amination process. Comparison of biosynthesis cost by reductive amination and transamination routes was carried out. Economic assessment based on the framework of cost analysis and preliminary process information revealed that cost is greatly dependent on enzyme price. The results indicated that enhancing the activity of amine dehydrogenase by 4–5 folds can drop the unit price of reductive amination to $0.5–0.6/g, which make it competitive with transamination route. |
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| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T14:08:54Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-7c602ebe1af0445ebc87e68d9937cbb72023-11-21T00:23:28ZengMDPI AGCatalysts2073-43442020-12-011012145110.3390/catal10121451Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme SystemHeyu Huo0Guangxiao Yao1Shizhen Wang2Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaChiral amines are key building blocks for pharmaceuticals. Economic assessment of commercial potential of bioprocesses is needed for guiding research. Biosynthesis of (S)-α-methylbenzylamine (MBA) was selected as case study. For transamination route, transaminase coupled with glucose dehydrogenase and lactate dehydrogenase catalyzed the reaction with NADH (Nicotinamide adenine dinucleotide) regeneration. Amine dehydrogenase coupled with NADH oxidase, which catalyzed the reductive amination process. Comparison of biosynthesis cost by reductive amination and transamination routes was carried out. Economic assessment based on the framework of cost analysis and preliminary process information revealed that cost is greatly dependent on enzyme price. The results indicated that enhancing the activity of amine dehydrogenase by 4–5 folds can drop the unit price of reductive amination to $0.5–0.6/g, which make it competitive with transamination route.https://www.mdpi.com/2073-4344/10/12/1451chiral amineamine dehydrogenasetransaminasemulti-enzyme systemeconomic estimationbioprocess |
| spellingShingle | Heyu Huo Guangxiao Yao Shizhen Wang Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System Catalysts chiral amine amine dehydrogenase transaminase multi-enzyme system economic estimation bioprocess |
| title | Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System |
| title_full | Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System |
| title_fullStr | Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System |
| title_full_unstemmed | Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System |
| title_short | Economy Assessment for the Chiral Amine Production with Comparison of Reductive Amination and Transamination Routes by Multi-Enzyme System |
| title_sort | economy assessment for the chiral amine production with comparison of reductive amination and transamination routes by multi enzyme system |
| topic | chiral amine amine dehydrogenase transaminase multi-enzyme system economic estimation bioprocess |
| url | https://www.mdpi.com/2073-4344/10/12/1451 |
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