Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection
Varieties of microorganisms reside in the oral cavity contributing to the occurrence and development of microbes associated with oral diseases; however, the distribution and in situ abundance in the biofilm are still unclear. In order to promote the understanding of the ecosystem of oral microbiota...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1450 |
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| author | Junjie Gu Huayu Wang Mengye Zhang Yichen Xiong Lei Yang Biao Ren Ruijie Huang |
| author_facet | Junjie Gu Huayu Wang Mengye Zhang Yichen Xiong Lei Yang Biao Ren Ruijie Huang |
| author_sort | Junjie Gu |
| collection | DOAJ |
| description | Varieties of microorganisms reside in the oral cavity contributing to the occurrence and development of microbes associated with oral diseases; however, the distribution and in situ abundance in the biofilm are still unclear. In order to promote the understanding of the ecosystem of oral microbiota and the diagnosis of oral diseases, it is necessary to monitor and compare the oral microorganisms from different niches of the oral cavity in situ. The fluorescence in situ hybridization (FISH) has proven to be a powerful tool for representing the status of oral microorganisms in the oral cavity. FISH is one of the most routinely used cytochemical techniques for genetic detection, identification, and localization by a fluorescently labeled nucleic acid probe, which can hybridize with targeted nucleic acid sequences. It has the advantages of rapidity, safety, high sensitivity, and specificity. FISH allows the identification and quantification of different oral microorganisms simultaneously. It can also visualize microorganisms by combining with other molecular biology technologies to represent the distribution of each microbial community in the oral biofilm. In this review, we summarized and discussed the development of FISH technology and the application of FISH in oral disease diagnosis and oral ecosystem research, highlighted its advantages in oral microbiology, listed the existing problems, and provided suggestions for future development.. |
| first_indexed | 2024-03-09T15:59:25Z |
| format | Article |
| id | doaj.art-7c6b59222e7046cd8f2ec6418ec4775c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:25Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7c6b59222e7046cd8f2ec6418ec4775c2023-11-24T17:12:55ZengMDPI AGPathogens2076-08172022-12-011112145010.3390/pathogens11121450Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial DetectionJunjie Gu0Huayu Wang1Mengye Zhang2Yichen Xiong3Lei Yang4Biao Ren5Ruijie Huang6State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, ChinaVarieties of microorganisms reside in the oral cavity contributing to the occurrence and development of microbes associated with oral diseases; however, the distribution and in situ abundance in the biofilm are still unclear. In order to promote the understanding of the ecosystem of oral microbiota and the diagnosis of oral diseases, it is necessary to monitor and compare the oral microorganisms from different niches of the oral cavity in situ. The fluorescence in situ hybridization (FISH) has proven to be a powerful tool for representing the status of oral microorganisms in the oral cavity. FISH is one of the most routinely used cytochemical techniques for genetic detection, identification, and localization by a fluorescently labeled nucleic acid probe, which can hybridize with targeted nucleic acid sequences. It has the advantages of rapidity, safety, high sensitivity, and specificity. FISH allows the identification and quantification of different oral microorganisms simultaneously. It can also visualize microorganisms by combining with other molecular biology technologies to represent the distribution of each microbial community in the oral biofilm. In this review, we summarized and discussed the development of FISH technology and the application of FISH in oral disease diagnosis and oral ecosystem research, highlighted its advantages in oral microbiology, listed the existing problems, and provided suggestions for future development..https://www.mdpi.com/2076-0817/11/12/1450fluorescence in situ hybridizationmicrobial detectionoral microecologyoral microorganism |
| spellingShingle | Junjie Gu Huayu Wang Mengye Zhang Yichen Xiong Lei Yang Biao Ren Ruijie Huang Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection Pathogens fluorescence in situ hybridization microbial detection oral microecology oral microorganism |
| title | Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection |
| title_full | Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection |
| title_fullStr | Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection |
| title_full_unstemmed | Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection |
| title_short | Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection |
| title_sort | application of fluorescence in situ hybridization fish in oral microbial detection |
| topic | fluorescence in situ hybridization microbial detection oral microecology oral microorganism |
| url | https://www.mdpi.com/2076-0817/11/12/1450 |
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