Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria
Abstract Background An alarm increase the rate of emerging and re-emerging of multidrug resistant bacteria have been caused great public health concern in the worldwide. They have been resisting for most or majority of currently available and affordable antibiotics and imposed socioeconomic catastro...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2429-4 |
| _version_ | 1818912802413215744 |
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| author | Eshetu Gadisa Gebru Weldearegay Kassu Desta Getahun Tsegaye Sityehu Hailu Kefiyelewu Jote Abera Takele |
| author_facet | Eshetu Gadisa Gebru Weldearegay Kassu Desta Getahun Tsegaye Sityehu Hailu Kefiyelewu Jote Abera Takele |
| author_sort | Eshetu Gadisa |
| collection | DOAJ |
| description | Abstract Background An alarm increase the rate of emerging and re-emerging of multidrug resistant bacteria have been caused great public health concern in the worldwide. They have been resisting for most or majority of currently available and affordable antibiotics and imposed socioeconomic catastrophe at global scale. As a result, there is utmost important to discover new or modify currently available antibiotics. The aim of this study was to evaluate combined antibacterial effect of essential oils obtained from Blepharis cuspidata, Boswellia ogadensis and Thymus schimper against multidrug resistance (MDR) Escherichia coli, Klebsiella pneumoniae and Methicillin resistant S. aureus. Methods Essential oil (EO) was extracted from the aerial part of B. cuspidata, B.ogadensis and T. schimper by steam distillation and stored in brown bottles at 4 °C. There were mixed in 1:1 ratio and adsorbed to disc and placed on MHA and measured their minimum inhibitory zone seeded with E. coli, K. pneumoniae and MRAS after 18-24 H. minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by broth micro-dilution method. The interaction between EOs was determined by fractional inhibitory concentration index. Results The antibacterial potential of mixed oil depends on the doses and type of the EOs and bacteria species. The combined EOs of B.cuspidata and T.schimperi had inhibition zone (39 mm), its MIC and MBC value was 0.39 μl/ml against MRSA. It had inhibition zone (28-35 mm), MIC value 0.39–6.25 μl/ml and MBC (0.78–12.5 μl/ml) against MDR E. coli and K. pneumoniae. Whereas, combined effects of B. cuspidata and B. ogadensis had MIC values ranges from 0.78–6.25 μl/ml for E.coli and K. pneumoniae and 1.56 μl/ml for MRSA. There was strong synergistic effect between the combination of B.cuspidata and T.schimperi. This study revealed that gram negative bacteria were slightly less susceptible than gram positive. Conclusions This in vitro study of combined EOs has significant antibacterial effect than using each of them and even it was more potent antibacterial effect on MDR as compare to modern antibiotics. Hence, it can be applied to a pharmaceutical composition as modulator or adjuvant or precursor for synthesis of new antibiotic in future activities. |
| first_indexed | 2024-12-19T23:20:23Z |
| format | Article |
| id | doaj.art-7c6ca585da6f425da91c5917c02f67b9 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-19T23:20:23Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-7c6ca585da6f425da91c5917c02f67b92022-12-21T20:02:00ZengBMCBMC Complementary and Alternative Medicine1472-68822019-01-011911910.1186/s12906-019-2429-4Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteriaEshetu Gadisa0Gebru Weldearegay1Kassu Desta2Getahun Tsegaye3Sityehu Hailu4Kefiyelewu Jote5Abera Takele6Kotebe Metropolitan UniversityDepartment of Medical Laboratory Science, CHS, AAUDepartment of Medical Laboratory Science, CHS, AAUDepartment of Human Physiology, CHS, AAUCHS, Madda Walabu UniversityKotebe Metropolitan UniversitySalale UniversityAbstract Background An alarm increase the rate of emerging and re-emerging of multidrug resistant bacteria have been caused great public health concern in the worldwide. They have been resisting for most or majority of currently available and affordable antibiotics and imposed socioeconomic catastrophe at global scale. As a result, there is utmost important to discover new or modify currently available antibiotics. The aim of this study was to evaluate combined antibacterial effect of essential oils obtained from Blepharis cuspidata, Boswellia ogadensis and Thymus schimper against multidrug resistance (MDR) Escherichia coli, Klebsiella pneumoniae and Methicillin resistant S. aureus. Methods Essential oil (EO) was extracted from the aerial part of B. cuspidata, B.ogadensis and T. schimper by steam distillation and stored in brown bottles at 4 °C. There were mixed in 1:1 ratio and adsorbed to disc and placed on MHA and measured their minimum inhibitory zone seeded with E. coli, K. pneumoniae and MRAS after 18-24 H. minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by broth micro-dilution method. The interaction between EOs was determined by fractional inhibitory concentration index. Results The antibacterial potential of mixed oil depends on the doses and type of the EOs and bacteria species. The combined EOs of B.cuspidata and T.schimperi had inhibition zone (39 mm), its MIC and MBC value was 0.39 μl/ml against MRSA. It had inhibition zone (28-35 mm), MIC value 0.39–6.25 μl/ml and MBC (0.78–12.5 μl/ml) against MDR E. coli and K. pneumoniae. Whereas, combined effects of B. cuspidata and B. ogadensis had MIC values ranges from 0.78–6.25 μl/ml for E.coli and K. pneumoniae and 1.56 μl/ml for MRSA. There was strong synergistic effect between the combination of B.cuspidata and T.schimperi. This study revealed that gram negative bacteria were slightly less susceptible than gram positive. Conclusions This in vitro study of combined EOs has significant antibacterial effect than using each of them and even it was more potent antibacterial effect on MDR as compare to modern antibiotics. Hence, it can be applied to a pharmaceutical composition as modulator or adjuvant or precursor for synthesis of new antibiotic in future activities.http://link.springer.com/article/10.1186/s12906-019-2429-4Blepharis cuspidataBoswellia ogadensisThymus schimperiMRSA |
| spellingShingle | Eshetu Gadisa Gebru Weldearegay Kassu Desta Getahun Tsegaye Sityehu Hailu Kefiyelewu Jote Abera Takele Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria BMC Complementary and Alternative Medicine Blepharis cuspidata Boswellia ogadensis Thymus schimperi MRSA |
| title | Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria |
| title_full | Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria |
| title_fullStr | Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria |
| title_full_unstemmed | Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria |
| title_short | Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria |
| title_sort | combined antibacterial effect of essential oils from three most commonly used ethiopian traditional medicinal plants on multidrug resistant bacteria |
| topic | Blepharis cuspidata Boswellia ogadensis Thymus schimperi MRSA |
| url | http://link.springer.com/article/10.1186/s12906-019-2429-4 |
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