Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
Summary: Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223015675 |
| _version_ | 1797743994731495424 |
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| author | Catherine Socha Inês S. Pais Kwang-Zin Lee Jiyong Liu Samuel Liégeois Matthieu Lestradet Dominique Ferrandon |
| author_facet | Catherine Socha Inês S. Pais Kwang-Zin Lee Jiyong Liu Samuel Liégeois Matthieu Lestradet Dominique Ferrandon |
| author_sort | Catherine Socha |
| collection | DOAJ |
| description | Summary: Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the regrowth of Drosophila enterocytes entails an inversion of metabolic fluxes from the organism back toward the intestine. We identify a proton-assisted transporter, Arcus, that is required for the reverse absorption of amino acids and the timely recovery of the intestinal epithelium. Arcus is required for a peak of amino acids appearing in the hemolymph shortly after infection. The regrowth of enterocytes involves the insulin signaling pathway and Myc. The purge decreases Myc mRNA levels, which subsequently remain at low levels in the arcus mutant. Interestingly, the action of arcus and Myc in the intestinal epithelium is not cell-autonomous, suggesting amino acid fluxes within the intestinal epithelium. |
| first_indexed | 2024-03-12T15:03:29Z |
| format | Article |
| id | doaj.art-7c7085756ff248a18d57aefcd570967f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T15:03:29Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-7c7085756ff248a18d57aefcd570967f2023-08-13T04:54:34ZengElsevieriScience2589-00422023-09-01269107490Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporterCatherine Socha0Inês S. Pais1Kwang-Zin Lee2Jiyong Liu3Samuel Liégeois4Matthieu Lestradet5Dominique Ferrandon6Université de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, FranceUniversité de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, FranceUniversité de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, FranceSino-French Hoffmann Institute, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou 511436, Guangdong Province, ChinaUniversité de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, France; Sino-French Hoffmann Institute, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou 511436, Guangdong Province, ChinaUniversité de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, FranceUniversité de Strasbourg, CNRS, RIDI UPR 9022, F67084 Strasbourg, France; Sino-French Hoffmann Institute, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou 511436, Guangdong Province, China; Corresponding authorSummary: Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the regrowth of Drosophila enterocytes entails an inversion of metabolic fluxes from the organism back toward the intestine. We identify a proton-assisted transporter, Arcus, that is required for the reverse absorption of amino acids and the timely recovery of the intestinal epithelium. Arcus is required for a peak of amino acids appearing in the hemolymph shortly after infection. The regrowth of enterocytes involves the insulin signaling pathway and Myc. The purge decreases Myc mRNA levels, which subsequently remain at low levels in the arcus mutant. Interestingly, the action of arcus and Myc in the intestinal epithelium is not cell-autonomous, suggesting amino acid fluxes within the intestinal epithelium.http://www.sciencedirect.com/science/article/pii/S2589004223015675Natural sciencesBiological sciencesZoologyBiochemistryParasitology |
| spellingShingle | Catherine Socha Inês S. Pais Kwang-Zin Lee Jiyong Liu Samuel Liégeois Matthieu Lestradet Dominique Ferrandon Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter iScience Natural sciences Biological sciences Zoology Biochemistry Parasitology |
| title | Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| title_full | Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| title_fullStr | Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| title_full_unstemmed | Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| title_short | Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| title_sort | fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter |
| topic | Natural sciences Biological sciences Zoology Biochemistry Parasitology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223015675 |
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