The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats
Background & Objective: Diabetes causes reproductive disorders by endocrine glands dysfunction. Also curcumin has antioxidant and hypoglycemic properties. The purpose of this study was to determine the effect of curcumin on pituitary-gonadal axis, DNA oxidative damage and antioxidant enzymes act...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fasa University of Medical Sciences
2017-12-01
|
| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1287-en.pdf |
| _version_ | 1797679632203382784 |
|---|---|
| author | Seyed Damoun Sadoughi Mohammad Amin Edalatmanesh Raheleh Rahbarian |
| author_facet | Seyed Damoun Sadoughi Mohammad Amin Edalatmanesh Raheleh Rahbarian |
| author_sort | Seyed Damoun Sadoughi |
| collection | DOAJ |
| description | Background & Objective: Diabetes causes reproductive disorders by endocrine glands dysfunction. Also curcumin has antioxidant and hypoglycemic properties. The purpose of this study was to determine the effect of curcumin on pituitary-gonadal axis, DNA oxidative damage and antioxidant enzymes activity of testicular tissue in male type one diabetic rats.
Material & Methods: In this experimental study 40 male Wistar rats were allocated into 5 equal groups (n=8). Control group, non-treated diabetic group (30 days, intraperitoneal injection of dimethyl sulfoxide) and treated diabetic groups with doses 50, 100 and 150 mg/kg of curcumin (30 days, intraperitoneal injection of curcumin dissolved in dimethyl sulfoxide). Diabetes was induced using an intraperitoneal injection of 240 mg/kg alloxan. At the end of treatments, serum levels of LH, FSH, estrogen, testosterone, dihydrotestosterone and levels of SOD, CAT, GPX, HOdG-8 and MDA in testicle tissue were measured by ELISA. The statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05).
Result: Compared to the non-treated diabetic group, administration of curcumin with doses of 100 and 150 mg/kg caused dose-dependent significant increased serum levels of LH, FSH, estrogen, testosterone and dihydrotestosterone, dose-dependent significant decreased HOdG-8 and MDA and also dose-dependent significant increased enzyme of SOD, CAT, and GPX in diabetic rats’ testicle (p<0.05).
Conclusion: Administration of curcumin increases pituitary-gonadal axis activity, decreases lipid peroxidation and DNA oxidative damage and also increases antioxidant enzyme activity of testicular tissue in diabetic rats. |
| first_indexed | 2024-03-11T23:17:37Z |
| format | Article |
| id | doaj.art-7c7302fe11fa4e6f9779c010317947d2 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:37Z |
| publishDate | 2017-12-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-7c7302fe11fa4e6f9779c010317947d22023-09-20T21:23:30ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232017-12-0174511520The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic RatsSeyed Damoun Sadoughi0Mohammad Amin Edalatmanesh1Raheleh Rahbarian2 Department of Biology, College of Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran Department of Biology, College of Sciences, Payam-e-Noor University, Tehran, Iran Background & Objective: Diabetes causes reproductive disorders by endocrine glands dysfunction. Also curcumin has antioxidant and hypoglycemic properties. The purpose of this study was to determine the effect of curcumin on pituitary-gonadal axis, DNA oxidative damage and antioxidant enzymes activity of testicular tissue in male type one diabetic rats. Material & Methods: In this experimental study 40 male Wistar rats were allocated into 5 equal groups (n=8). Control group, non-treated diabetic group (30 days, intraperitoneal injection of dimethyl sulfoxide) and treated diabetic groups with doses 50, 100 and 150 mg/kg of curcumin (30 days, intraperitoneal injection of curcumin dissolved in dimethyl sulfoxide). Diabetes was induced using an intraperitoneal injection of 240 mg/kg alloxan. At the end of treatments, serum levels of LH, FSH, estrogen, testosterone, dihydrotestosterone and levels of SOD, CAT, GPX, HOdG-8 and MDA in testicle tissue were measured by ELISA. The statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05). Result: Compared to the non-treated diabetic group, administration of curcumin with doses of 100 and 150 mg/kg caused dose-dependent significant increased serum levels of LH, FSH, estrogen, testosterone and dihydrotestosterone, dose-dependent significant decreased HOdG-8 and MDA and also dose-dependent significant increased enzyme of SOD, CAT, and GPX in diabetic rats’ testicle (p<0.05). Conclusion: Administration of curcumin increases pituitary-gonadal axis activity, decreases lipid peroxidation and DNA oxidative damage and also increases antioxidant enzyme activity of testicular tissue in diabetic rats.http://jabs.fums.ac.ir/article-1-1287-en.pdfdiabetes mellituscurcumintestisantioxidant enzymesrat |
| spellingShingle | Seyed Damoun Sadoughi Mohammad Amin Edalatmanesh Raheleh Rahbarian The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats Journal of Advanced Biomedical Sciences diabetes mellitus curcumin testis antioxidant enzymes rat |
| title | The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats |
| title_full | The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats |
| title_fullStr | The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats |
| title_full_unstemmed | The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats |
| title_short | The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats |
| title_sort | effect of curcumin on pituitary gonadal axis dna oxidative damage and antioxidant enzymes activity of testicular tissue in male diabetic rats |
| topic | diabetes mellitus curcumin testis antioxidant enzymes rat |
| url | http://jabs.fums.ac.ir/article-1-1287-en.pdf |
| work_keys_str_mv | AT seyeddamounsadoughi theeffectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats AT mohammadaminedalatmanesh theeffectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats AT rahelehrahbarian theeffectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats AT seyeddamounsadoughi effectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats AT mohammadaminedalatmanesh effectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats AT rahelehrahbarian effectofcurcuminonpituitarygonadalaxisdnaoxidativedamageandantioxidantenzymesactivityoftesticulartissueinmalediabeticrats |