TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15
Background: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/503 |
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| author | Alice Elena Ghenea Ovidiu Mircea Zlatian Oana Mariana Cristea Anca Ungureanu Radu Razvan Mititelu Andrei Theodor Balasoiu Corina Maria Vasile Alex-Ioan Salan Daniel Iliuta Mihaela Popescu Anca-Loredana Udriștoiu Maria Balasoiu |
| author_facet | Alice Elena Ghenea Ovidiu Mircea Zlatian Oana Mariana Cristea Anca Ungureanu Radu Razvan Mititelu Andrei Theodor Balasoiu Corina Maria Vasile Alex-Ioan Salan Daniel Iliuta Mihaela Popescu Anca-Loredana Udriștoiu Maria Balasoiu |
| author_sort | Alice Elena Ghenea |
| collection | DOAJ |
| description | Background: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatment, increases mortality and morbidity in patients, and leads to longer hospitalization and expensive costs. We aimed to identify the beta-lactamases circulating encoded by the genes <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>SHV-1</sub> and <i>bla</i><sub>TEM-1</sub> in <i>Escherichia coli (E. coli)</i> and <i>Klebsiella pneumoniae (K. pneumoniae)</i> strains. Furthermore, we established the associated resistance phenotypes among patients hospitalized in the Intensive Care Unit (ICU) from County Clinical Emergency Hospital of Craiova, Romania. Methods: A total of 46 non-duplicated bacterial strains (14 strains of <i>E. coli</i> and 32 strains of <i>K. pneumoniae</i>), which were resistant to ceftazidime (CAZ) and cefotaxime (CTX) by Kirby–Bauer disk diffusion method, were identified using the automated VITEK2 system. Detection of ESBL-encoding genes and other resistance genes was carried out by PCR. Results. <i>E. coli</i> strains were resistant to 3rd generation cephalosporins and moderately resistant to quinolones, whereas <i>K. pneumoniae</i> strains were resistant to penicillins, cephalosporins, and sulfamides, and moderately resistant to quinolones and carbapenems. Most <i>E. coli</i> strains harbored <i>bla</i><sub>CTX-M-15</sub> gene (13/14 strains), a single strain had the <i>bla</i><sub>SHV-1</sub> gene, but 11 strains harbored <i>bla</i><sub>TEM-1</sub> gene. The <i>mcr-1</i> gene was not detected. We detected <i>tet</i>(A) gene in six strains and <i>tet</i>(B) in one strain. In <i>K. pneumoniae</i> strains we detected <i>bla</i><sub>CTX-M-15</sub> in 23 strains, <i>bla</i><sub>SHV</sub>-<sub>1</sub> in all strains and <i>bla</i><sub>TEM-1</sub> in 14 strains. The colistin resistance gene <i>mcr-1</i> was not detected. The tetracycline gene <i>tet</i>(A) was detected in 11 strains, but the gene <i>tet</i>(B) was not detected in any strains. Conclusions. The development in antibiotic resistance highlights the importance of establishing policies to reduce antibiotic use and improving the national resistance surveillance system in order to create local antibiotic therapy guidelines. |
| first_indexed | 2024-03-09T11:15:03Z |
| format | Article |
| id | doaj.art-7c7630b8b4fa4fe38993cd3ea07db8da |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:15:03Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-7c7630b8b4fa4fe38993cd3ea07db8da2023-12-01T00:32:59ZengMDPI AGAntibiotics2079-63822022-04-0111450310.3390/antibiotics11040503TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15Alice Elena Ghenea0Ovidiu Mircea Zlatian1Oana Mariana Cristea2Anca Ungureanu3Radu Razvan Mititelu4Andrei Theodor Balasoiu5Corina Maria Vasile6Alex-Ioan Salan7Daniel Iliuta8Mihaela Popescu9Anca-Loredana Udriștoiu10Maria Balasoiu11Department of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Ophthalmology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Paediatrics, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Oral and Maxillofacial Surgery, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Psychiatry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Endocrinology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaFaculty of Automation, Computers and Electronics, University of Craiova, 200776 Craiova, RomaniaDepartment of Bacteriology-Virology-Parasitology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaBackground: CTX-M betalactamases have shown a rapid spread in the recent years among Enterobacteriaceae and have become the most prevalent Extended Spectrum Beta-Lactamases (ESBLs) in many parts of the world. The introduction and dissemination of antibiotic-resistant genes limits options for treatment, increases mortality and morbidity in patients, and leads to longer hospitalization and expensive costs. We aimed to identify the beta-lactamases circulating encoded by the genes <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>SHV-1</sub> and <i>bla</i><sub>TEM-1</sub> in <i>Escherichia coli (E. coli)</i> and <i>Klebsiella pneumoniae (K. pneumoniae)</i> strains. Furthermore, we established the associated resistance phenotypes among patients hospitalized in the Intensive Care Unit (ICU) from County Clinical Emergency Hospital of Craiova, Romania. Methods: A total of 46 non-duplicated bacterial strains (14 strains of <i>E. coli</i> and 32 strains of <i>K. pneumoniae</i>), which were resistant to ceftazidime (CAZ) and cefotaxime (CTX) by Kirby–Bauer disk diffusion method, were identified using the automated VITEK2 system. Detection of ESBL-encoding genes and other resistance genes was carried out by PCR. Results. <i>E. coli</i> strains were resistant to 3rd generation cephalosporins and moderately resistant to quinolones, whereas <i>K. pneumoniae</i> strains were resistant to penicillins, cephalosporins, and sulfamides, and moderately resistant to quinolones and carbapenems. Most <i>E. coli</i> strains harbored <i>bla</i><sub>CTX-M-15</sub> gene (13/14 strains), a single strain had the <i>bla</i><sub>SHV-1</sub> gene, but 11 strains harbored <i>bla</i><sub>TEM-1</sub> gene. The <i>mcr-1</i> gene was not detected. We detected <i>tet</i>(A) gene in six strains and <i>tet</i>(B) in one strain. In <i>K. pneumoniae</i> strains we detected <i>bla</i><sub>CTX-M-15</sub> in 23 strains, <i>bla</i><sub>SHV</sub>-<sub>1</sub> in all strains and <i>bla</i><sub>TEM-1</sub> in 14 strains. The colistin resistance gene <i>mcr-1</i> was not detected. The tetracycline gene <i>tet</i>(A) was detected in 11 strains, but the gene <i>tet</i>(B) was not detected in any strains. Conclusions. The development in antibiotic resistance highlights the importance of establishing policies to reduce antibiotic use and improving the national resistance surveillance system in order to create local antibiotic therapy guidelines.https://www.mdpi.com/2079-6382/11/4/503antimicrobial resistance<i>Klebsiella pneumoniae</i><i>Escherichia coli</i>CTX-M-15ESBLantibiotic resistance |
| spellingShingle | Alice Elena Ghenea Ovidiu Mircea Zlatian Oana Mariana Cristea Anca Ungureanu Radu Razvan Mititelu Andrei Theodor Balasoiu Corina Maria Vasile Alex-Ioan Salan Daniel Iliuta Mihaela Popescu Anca-Loredana Udriștoiu Maria Balasoiu TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 Antibiotics antimicrobial resistance <i>Klebsiella pneumoniae</i> <i>Escherichia coli</i> CTX-M-15 ESBL antibiotic resistance |
| title | TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 |
| title_full | TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 |
| title_fullStr | TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 |
| title_full_unstemmed | TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 |
| title_short | TEM,CTX-M,SHV Genes in ESBL-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples in a County Clinical Emergency Hospital Romania-Predominance of CTX-M-15 |
| title_sort | tem ctx m shv genes in esbl producing i escherichia coli i and i klebsiella pneumoniae i isolated from clinical samples in a county clinical emergency hospital romania predominance of ctx m 15 |
| topic | antimicrobial resistance <i>Klebsiella pneumoniae</i> <i>Escherichia coli</i> CTX-M-15 ESBL antibiotic resistance |
| url | https://www.mdpi.com/2079-6382/11/4/503 |
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